Effect of CRP and its ARI mutant CRP on the p1p2 expression in the double mutant TP2339-1 detected by primer extension analysis

<p><b>Copyright information:</b></p><p>Taken from "Interplay between CRP-cAMP and PII-Ntr systems forms novel regulatory network between carbon metabolism and nitrogen assimilation in "</p><p></p><p>Nucleic Acids Research 2007;35(5):1432-1440.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865078.</p><p>© 2007 The Author(s).</p> Lane 5 is G marker ladder. Cells were grown in M63 minimal medium with glycerol (0.4% w/v) as the sole carbon source, and glutamine (0.2% w/v) as nitrogen source, in the absence/presence of exogenous cAMP (2 mM)

Effect of CRP and its ARI mutant CRP on the uridylylation status of GlnB in the double mutant TP2339-1 and triple mutant BD4000

<p><b>Copyright information:</b></p><p>Taken from "Interplay between CRP-cAMP and PII-Ntr systems forms novel regulatory network between carbon metabolism and nitrogen assimilation in "</p><p></p><p>Nucleic Acids Research 2007;35(5):1432-1440.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865078.</p><p>© 2007 The Author(s).</p> All strains (lanes 1–8) were grown in M63 minimal medium with glycerol (0.4% w/v) as the sole carbon source, and glutamine (0.2% w/v) as nitrogen source, in the absence/presence of exogenous cAMP (2 mM). TP2339-1 was grown in nitrogen-sufficient medium with 40 mM ammonium as control (lane 9)

DNase I footprints of Eσ in the presence or absence of CRP and CRP on p1 (non template strand)

<p><b>Copyright information:</b></p><p>Taken from "Interplay between CRP-cAMP and PII-Ntr systems forms novel regulatory network between carbon metabolism and nitrogen assimilation in "</p><p></p><p>Nucleic Acids Research 2007;35(5):1432-1440.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865078.</p><p>© 2007 The Author(s).</p> () Titration with increasing concentrations of CRP (lanes 2 and 7, 30 nM; lanes 3 and 8, 100 nM; lanes 4 and 9, 300 nM) were performed in the absence (2–4) or presence of 50 nM Eσ (lanes 7–9). Lane 11 is A + G marker ladder. The protected regions were monitored by adding increasing concentrations of CRP in the presence or absence of Eσ. () Titration with increasing concentrations of CRP (lane 3, 33 nM; lane 4, 100 nM; lane 5, 300 nM) and CRP (lane 10, 33 nM; lane 11, 100 nM; lane 12, 300 nM) were performed in the presence of 25 nM Eσ (lanes 3–5 and 10–12). Lane 7 is A + G marker ladder. The limits of protected regions are indicated. Note that wild-type CRP is recruited to a site centred at −61.5 by Eσ-RNA polymerase, which suggests a higher affinity for DNA binding of Eσ than that of CRP