Dispersal patterns of endogenous bacteria among grass carp (Ctenopharyngodon idellus) guts

The formation and regulation of vertebrate endogenous intestinal microbiota has been widely studied as the microbiota plays a crucial role in the host nutrition, development, and health. Despite the importance of microbiota for host health, it is still unclear whether the endogenous intestinal microorganisms are genetically distinct or whether they are genetically related with each other in different host individuals. In the present study, the dispersal situation of the endogenous intestinal bacteria in grass carp was investigated by constructing bacterial 16S rRNA gene clone libraries. The results indicate that the bacteria harbored in the grass carp gut could be separated into the following two groups: a- the private operational taxonomic units (OTUs), which include Cetobacterium somerae, Aeromonas jandaei, Citrobacter freundii, Achromobacter xylosoxidans and Bacteroides species; b- the shared OTUs, which include Vibrio cholerae, Plesiomonas shigelloides and Pasteurella speices. The results obtained in this investigation provide valuable information for assessing the mechanism of spread of the endogenous intestinal bacteria, especially the pathogenic ones. However, the mechanisms involved in different modes of bacterial dispersal in the grass carp gut still require further research

Using a novel petroselinic acid embedded cellulose acetate membrane to mimic plant partitioning and in vivo uptake of polycyclic aromatic hydrocarbons

A new type of composite membrane is introduced to mimic plant uptake of hydrophobic organic contaminants (HOCs). Petroselinic acid (cis-6-octadecenoic acid),the major component of plant lipids, was embedded in the matrix of cellulose acetate polymer to form the petroselinic acid embedded cellulose acetate membrane (PECAM). Accumulation of the polycyclic aromatic hydrocarbons (PAHs) naphthalene (Nap), phenanthrene (Phe), pyrene (Pyr), and benz(a)pyrene (Bap) by PECAM was compared with their uptake by plants. The accumulation of Nap, Phe, Pyr, and Bap by PECAM reached equilibrium in 24,48,144, and 192 h, respectively. The petroselinic acid-water partition coefficients (log K(pw), 3.37, 4.90, 5.24, and 6.28 for Nap, Phe, Pyr, and Bap, respectively) were positively correlated with the hydrophobicity of the compounds (R(2) = 0.995) and were almost the same as the lipid-normalized root partition coefficients (log K(lip)) for the corresponding compounds. Their relationship can be expressed as log K(pw) = 0.98 log K(lip). The normalized plant uptake coefficients (log K(u)) obtained by in vivo experiments with a range of plant species (2.92, 4.43, 5.06, and 6.13 on average for Nap, Phe, Pyr, and Bap, respectively) were slightly lower than those of the log K(pw) values for the corresponding compounds, presumably due to their acropetal translocation and biodegradation inside plants. This work suggests that PECAMs can well mimic plant partitioning and in vivo uptake of PAHs and may have good potential as a nonliving accumulator to mimic plant uptake of PAHs and perhaps other HOCs

Discovery of C-glycosylpyranonaphthoquinones in Streptomyces sp. MBT76 by a combined NMR-based metabolomics and bioinformatics workflow

Article / Letter to editorInstituut Biologie Leide

Discovery of C-glycosylpyranonaphthoquinones in Streptomyces sp. MBT76 by a combined NMR-based metabolomics and bioinformatics workflow

Article / Letter to editorInstituut Biologie Leide

Discovery of C-glycosylpyranonaphthoquinones in Streptomyces sp. MBT76 by a combined NMR-based metabolomics and bioinformatics workflow

Article / Letter to editorInstituut Biologie Leide

Discovery of C-glycosylpyranonaphthoquinones in Streptomyces sp. MBT76 by a combined NMR-based metabolomics and bioinformatics workflow

Article / Letter to editorInstituut Biologie Leide

Discovery of C-glycosylpyranonaphthoquinones in Streptomyces sp. MBT76 by a combined NMR-based metabolomics and bioinformatics workflow

Article / Letter to editorInstituut Biologie Leide