High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.

Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes. To date, studies have been published that use small panels of iPSC-derived cell lines to study monogenic diseases. However, to study complex diseases, where the genetic variation underlying the disorder is unknown, a sizable number of patient-specific iPSC lines and controls need to be generated. Currently the methods for deriving and characterizing iPSCs are time consuming, expensive, and, in some cases, descriptive but not quantitative. Here we set out to develop a set of simple methods that reduce cost and increase throughput in the characterization of iPSC lines. Specifically, we outline methods for high-throughput quantification of surface markers, gene expression analysis of in vitro differentiation potential, and evaluation of karyotype with markedly reduced cost

A study of the spatial distributions of fast neutrons in lattices of slightly enriched uranium rods moderated by heavy water

"MIT-2344-5."Includes bibliographical references (pages 201-207)Contract AT(30-1)234

Longitudinal Modeling of Age-Related Memory Decline and the Apoe E4 Effect

BACKGROUND: The APOE ε4 allele is associated with the risk of late-onset Alzheimer\u27s disease. The age at which memory decline diverges among persons who are homozygous for the APOE ε4 allele, those who are heterozygous for the allele, and noncarriers is unknown. METHODS: Using local advertisements, we recruited cognitively normal subjects between the ages of 21 and 97 years, who were grouped according to their APOE ε4 status. We then followed the subjects with longitudinal neuropsychological testing. Anyone in whom mild cognitive impairment or dementia developed during follow-up was excluded. We compared the rates of decline in predetermined cognitive measures between carriers and noncarriers of the APOE ε4 allele, using a mixed model for longitudinal change with age. RESULTS: We analyzed 815 subjects: 317 APOE ε4 carriers (79 who were homozygous for the APOE ε4 allele and 238 who were heterozygous) and 498 noncarriers. Carriers, as compared with noncarriers, were generally younger (mean age, 58.0 vs. 61.4 years; P\u3c0.001) and were followed for a longer period (5.3 vs. 4.7 years, P=0.01), with an equivalent duration of formal education (15.4 years) and proportion of women (69%). Longitudinal decline in memory in carriers began before the age of 60 years and showed greater acceleration than in noncarriers (P=0.03), with a possible allele-dose effect (P=0.008). We observed similar although weaker effects on measures of visuospatial awareness and general mental status. CONCLUSIONS: Age-related memory decline in APOE ε4 carriers diverges from that of noncarriers before the age of 60 years, despite ongoing normal clinical status

Heavy water lattice project annual report / editors: Irving Kaplan, D.D. Lanning, T.J. Thompson ; contributors H. E. Bliss ... [et al.]

Statement of responsibility on title-page reads: editors: Irving Kaplan, D.D.Lanning, T.J. Thompson; contributors: H. E. Bliss, F.M. Clikeman, W.H. D'Ardenne, J.W. Gosnell, J. Harrington, III, I.Kaplan, H. Kim, D.D. Lanning, B.E.Malaviya, E.E. Pilat, A.E. Profio, E. Sefchovich. R. Simms, T.J. Thompson, G.L. Woodruff"September 30, 1963"Includes bibliographical referencesAnnual report; September 30, 1963U.S. Atomic Energy Commission contract AT(30-1)234

Heavy water lattice project annual report / editors: D.D. Lanning, I/ Kaplan [and] F.M. Clickeman ; contributors J. Barch ... [et al.]

Statement of responsibility on title-page reads: "Editors: D.D. Lanning, I. Kaplan, F.M. Clikeman; Contributors: J. Barch, N. Berube, F.M. Clikeman, W.H. D'Ardenne, J.W. Gosnell, J. Harrington III, I. Kaplan, B. Kelley, D.D. Lanning, B.K. Malaviya, L.T. Papay, E.E. Pilat, E. Sefchovich, A. Supple, T.J. Thompson, and G.L. Woodruff.""September 30, 1964.""MIT-2344-3."Includes bibliographical references (pages 165-168)Annual report; September 30, 1964U.S. Atomic Energy Commission contract AT(30-1)234

Heavy water lattice project annual report / editors: T.J. Thompson ... [et al.] ; contributors J. Barch ... [et al.]

Statement of responsibility on title-page reads: "Editors: T. J. Thompson, I. Kaplan, F.M. Clikeman, M.J. Driscoll; Contributors: J. Barch, N. Berube, H. Bliss, F.M. Clikeman, W.H. D'Ardenne, M.J. Driscoll, D.M. Goebel, J.W. Gosnell, H.M. Guéron, J. Harrington, III, S.P. Hellman, I. Kaplan, B. Kelley, D.D. Lanning, L.T. Papay, E.E, Pilat, C. Robertson, E. Sefchovich A. Supple, T.J. Thompson, and G.L. Woodruff .""September 30, 1965""MIT-2344-4."Includes bibliographical referencesAnnual report; September 30, 1965U.S. Atomic Energy Commission contract AT(30-1)234