Quantitative X-ray microradiography for high-throughput phenotyping of osteoarthritis in mice

Objective To investigate and validate digital X-ray microradiography as a novel, high-throughput and cost-effective screening approach to identify abnormal joint phenotypes in mice. Method Digital X-ray microradiography was used to quantify the subchondral bone mineral content (BMC) in the medial tibial plateau. Accuracy and reproducibility of the method were determined in 22 samples from C57BL/6(B6Brd;B6Dnk;B6N-Tyrc-Brd) wild-type mice. The method was then validated in wild-type mice that had undergone surgical destabilisation of medial meniscus (DMM) and in a genetically modified mouse strain with an established increase in trabecular bone mass. Results The measurement of subchondral BMC by digital X-ray microradiography had a coefficient of variation of 3.6%. Digital X-ray microradiography was able to demonstrate significantly increased subchondral BMC in the medial tibial plateau of male mice 4 and 8 weeks after DMM surgery and in female mice 8 weeks after surgery. Furthermore, digital X-ray microradiography also detected the increase in subchondral BMC in a genetically modified mouse strain with high trabecular bone mass. Conclusion Quantitation of subchondral BMC by digital X-ray microradiography is a rapid, sensitive and cost-effective method to identify abnormal joint phenotypes in mice of both genders at several ages

Fracture mechanics testing for environmental stress cracking in thermoplastics

Under the combined influence of an aggressive environment and applied stress, engineering thermoplastics may undergo a phenomenon known as environmental stress cracking (ESC). This can result in adverse effects such as embrittlement and premature failure in service, due to the growth of environmentally-induced cracks to critical sizes, with little to no fluid absorption in the bulk material. Fracture mechanics is proposed as a suitable scheme to study and quantify ESC, with the aim being to obtain characterising data for different polymer-fluid combinations of interest, as well as to develop a reliable fracture mechanics test protocol. In the proposed method, slow crack growth is monitored to assess the effect of a range of applied crack driving forces (K, or alternatively G) on observed crack speeds, as opposed to simply measuring time-to-failure. This paper presents the results of experiments performed on the following materials: linear low density polyethylene (LLDPE) in Igepal solution and high impact polystyrene (HIPS) in sunflower oil. A discussion of the various issues surrounding the data analysis for these long-term tests is also included, as the attainment of consistent and repeatable results is critical for a method to be internationally standardised, which is a goal of the European Structural Integrity Society (ESIS) Technical Committee 4 from whose interest this work is drawn

A meta-analysis of structural MRI studies of the brain in systemic lupus erythematosus (SLE)

A comprehensive search of published literature in brain volumetry was conducted in three autoimmune diseases — systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and ulcerative colitis (UC) — with the intention of performing a meta-analysis of published data. Due to the lack of data in RA and UC, the reported meta-analysis was limited to SLE. The MEDLINE database was searched for studies from 1988 to March 2022. A total of 175 papers met the initial inclusion criteria, and 16 were included in a random-effects meta-analysis. The reduction in the number of papers included in the final analysis is primarily due to the lack of overlap in measured and reported brain regions. A significantly lower volume was seen in patients with SLE in the hippocampus, corpus callosum, and total gray matter volume measurements as compared to age- and sex-matched controls. There were not enough studies to perform a meta-analysis for RA and UC; instead, we include a summary of published volumetric studies. The meta-analyses revealed structural brain abnormalities in patients with SLE, suggesting that lower global brain volumes are associated with disease status. This volumetric difference was seen in both the hippocampus and corpus callosum and total gray matter volume measurements. These results indicate both gray and white matter involvements in SLE and suggest there may be both localized and global reductions in brain volume

Experimental Tests of General Relativity

Einstein's general theory of relativity is the standard theory of gravity, especially where the needs of astronomy, astrophysics, cosmology and fundamental physics are concerned. As such, this theory is used for many practical purposes involving spacecraft navigation, geodesy, and time transfer. Here I review the foundations of general relativity, discuss recent progress in the tests of relativistic gravity in the solar system, and present motivations for the new generation of high-accuracy gravitational experiments. I discuss the advances in our understanding of fundamental physics that are anticipated in the near future and evaluate the discovery potential of the recently proposed gravitational experiments.Comment: revtex4, 30 pages, 10 figure