Additional file 1 of Phenotypic and metabolomic characteristics of mouse models of metabolic associated steatohepatitis

Additional file 1: Supplemental Fig. 1. Trends of total metabolites in mouse models of diet-induced metabolic dysfunction-associated steatohepatitis. (A) Plot generated through principal component analysis (PCA) of the total metabolites of mice fed with NC, HFD, WD, or HFC (16 and 32 wks). PC1: principal component 1; PC2: principal component 2. Each point represents the metabolite profile of a biological replicate. (B) Heatmap exhibiting prominent differences in total metabolites patterns among NC, 16-wk experimental diet, and 32-wk experimental diet. NC: normal chow; HFD: high-fat diet; WD: Western diet; and HFC: high-fat, high-cholesterol diet. Supplemental Table 1. List of real-time PCR primer. Supplemental Table 2. List of all metabolite in human. Supplemental Table 3. List of all lipoprotein subclass of mouse. Supplemental Table 4. List of small metabolites of mouse. Supplemental Table 5. Baseline characteristics of the study cohort

Down-regulated proteins in ERβ^-/- mouse urethra.

<p>Down-regulated proteins in ERβ^-/- mouse urethra.</p

Genotyping of different ERβ mutant mice.

<p>Lane 1, ERβ^+/+ mice; Lane 2, ERβ^+/- mice; Lane 3, ERβ^-/- mice.</p

Decreased urodynamic testing in ERβ^−/− mice.

<p>(A) LPP and (B) MUCP values in the different groups. Each bar represents the mean ± standard deviation of six individual mice. *<i>P</i><0.05 different from the value in the control group. **<i>P</i><0.01 different from the value in the control group.</p

Urethral dysfunction-related proteins expressions.

<p>Alterations of (A) myosin, (B) collagen, and (C) elastin expressions in urethra as indicated by Western blot analyses. The values are calculated by intensity of each band (ratio of target protein/GAPDH) and expressed as mean ± standard deviation of six individual mice. *Significantly different from the value in the control group (<i>P</i><0.05), ** <i>P</i><0.01.</p