Rare appearance of Candida tropicalis infection of the brain: Multiple micro-abscesses combined with diffuse hemorrhages

AbstractWe report a case of cerebral Candida tropicalis infection in a middle-aged patient who suffered from multiple cerebral micro-abscesses associated with diffuse hemorrhage due to perforation of esophagus. MRI revealed multiple irregular, nodular, ring-like enhancing lesions with restricted diffusion and multiple micro-hemorrhages as well as some leptomeningeal enhancements. Blood, sputum and urine cultures showed Candida tropicalis. The lesions were resolved after the patient was given early and effective treatment of anti-fungal medicine. The imaging findings provided limited differential diagnosis, leading to early diagnosis and treatment for this patient

The Design and Implementation of Collaborative Filtering in Data Mining

Data mining is the process of discovering explicit knowledge from large amounts of data stored in database, data warehouse or other repositories. There have been many studies about models of data mining such as association rule, sequential pattern and so on. Collaborative filtering is one of data mining models. In this paper, we propose two approaches to solving the mining process of collaborative filtering. Finally, collaborative filtering mining is applied to Knowledge Management system

MiRNA-145 increases therapeutic sensibility to gemcitabine treatment of pancreatic adenocarcinoma cells.

Pancreatic adenocarcinoma is one of the most leading causes of cancer-related deaths worldwide. Although recent advances provide various treatment options, pancreatic adenocarcinoma has poor prognosis due to its late diagnosis and ineffective therapeutic multimodality. Gemcitabine is the effective first-line drug in pancreatic adenocarcinoma treatment. However, gemcitabine chemoresistance of pancreatic adenocarcinoma cells has been a major obstacle for limiting its treatment effect. Our study found that p70S6K1 plays an important role in gemcitabine chemoresistence. MiR-145 is a tumor suppressor which directly targets p70S6K1 for inhibiting its expression in pancreatic adenocarcinoma, providing new therapeutic scheme. Our findings revealed a new mechanism underlying gemcitabine chemoresistance in pancreatic adenocarcinoma cells

(S)-(+)-4-(Oxiran-2-ylmeth­oxy)-9H-carbazole

In the title compound, C15H13NO2, all atoms of the carbazole group are coplanar (r.m.s. deviation = 0.005 Å), and the dihedral angle between this plane and C—O—C plane of oxane group is 57.1 (4)°. The crystal packing is stabilized by an N—H⋯O hydrogen bond, resulting in infinite supra­molecular chains along [001]

Cold hardiness of Phauda flammans (Lepidoptera: Zygaenidae) larvae

This study aimed to determine the cold hardiness of Phauda flammans (Lepidoptera: Zygaenidae) larvae. Supercooling points of the 1st–6th instar larvae of P. flammans ranged from –7.7 to –13.0 °C. The lethal temperatures were –8 °C for 1st, –5 °C for 2nd, and –7 °C for 3rd–6th instars. Lethal times at the instar-specific lethal temperatures were 12 h for 1st, 14 h for 2nd, 15 h for 3rd, 17 h for 4th, and 18 h for 5th–6th instars. The times required for all larvae to die in an incubator at 5 °C were 30 d for 1st, 3rd, 4th, and 5th instars, and 25 d for 2nd and 6th instars. The findings suggest that P. flammans is a chill-intolerant species, and larvae will die if the air temperature decreases to –5 to –8 °C for 12–18 h or to 5 °C for 25–30 d. Such conditions are, however, unlikely to occur in southern China

Complete genome sequencing and analysis of six enterovirus 71 strains with different clinical phenotypes

BACKGROUND: Hand, foot and mouth diseases (HFMD) caused by enterovirus 71(EV71) presents a broad spectrum of clinical manifestations ranging from mild febrile disease to fatal neurolocal disease. However, the mechanism of virulence is unknown. METHODS: We isolated 6 strains of EV71 from HFMD patients with or without neurological symptoms, and sequenced the whole genomes of the viruses to reveal the virulence factors of EV71. RESULTS: Phylogenetic tree based on VP1 region showed that all six strains clustered into C4a of C4 sub-genotype. In the complete polypeptide, 298 positions were found to be variable in all strains, and three of these positions (Val(P814)/Ile(P814) in VP1, Val(P1148)/Ile(P1148) in 3A and Ala (P1728)/Cys (P1728)/Val (P1728) in 3C) were conserved among the strains with neurovirulence, but variable in strains without neurovirulence. In the 5(′)-UTR region, it showed that the first 10 nucleotides were mostly conserved, however from the 11th nucleotide, nucleotide insertions and deletions were quite common. The secondary structure prediction of 5(′)-UTR sequences showed that two of three strains without neurovirulence (SDLY11 and SDLY48) were almost the same, and all strains with neurovirulence (SDLY96, SDLY107 and SDLY153) were different from each other. SDLY107 (a fatal strain) was found different from other strains on four positions (C(P241)/T(P241), A(P571)/T(P571), C(P579)/T(P579) in 5(′)-UTR and T(P7335)/C(P7335) in 3(′)-UTR). CONCLUSIONS: The three positions (Val(P814)/Ile(P814) in VP1, Val(P1148)/Ile(P1148) in 3A and Ala (P1728)/Cys (P1728)/Val (P1728) in 3C), were different between two phenotypes. These suggested that the three positions might be potential virulent positions. And the three varied positions were also found to be conserved in strains with neurovirulence, and variable in strains without neurovirulence. These might reveal that the conservation of two of the three positions or the three together were specific for the strains with neurovirulence. Varation of secondary structure of 5(′)-UTR, might be correlated to the changes of viral virulence. SDLY107 (a fatal strain) was found different from other strains on four positions, these positions might be related with death