Relative lengths and arm ratios of chromosomes of <i>H. discus hannai</i>.

<p>m: metacentric. sm: submetacentric. st: submetacentric.</p><p>Relative lengths and arm ratios of chromosomes of <i>H. discus hannai</i>.</p

Ag-nucleoli and 18S gene of interphase cells.

<p>a: 4 NORs in prophase with 2 normal NORs and 2 conglomerate NORs composed of many small NORs. b: 5 NORs on metaphase with 2 NORs composed with of small NORs. c: 18S rDNA on prophase cell after FISH. d: FISH with 18S gene on metaphase cell.</p

18S rDNA location on chromosome of <i>H. discus hannai</i>.

<p>a: Mitotic metaphase chromosomes after FISH treatment with the telomeric sequence (CCCTAA)_n. b: Three breaks on prophase chromosome of <i>H. discus hannai</i>, white arrows indicate two breaks in the centromere region and the green arrow indicates the terminal break. c: A rare karyotype with st chromosome, red arrow indicated st chromosome. (cy₃ shown as red color and DAPI shown as blue color.)</p

Karyotypes and NOR-bearing chromosomes of <i>H. discus hannai</i>.

<p>a: Digital image of metaphase plate from larvae of <i>H. discus hannai</i>. b: Karyotype of <i>H. discus hannai</i>. c: A metaphase of <i>H. discus hannai</i> with a short arm terminally NOR-bearing chromosome. d: A metacentric chromosome with short arm terminally NOR-bearing and a submetacentric chromosome with its short arm terminally NOR-bearing. (The arrows indicate zoom from chromosomes from parts c and b.)</p

Phylogenetic tree analysis of <i>Ca</i>-Chit from <i>C</i>. <i>angulata</i> with members of the GH18 family using MEGA 4.0.

<p>The amino acid sequence alignment was carried out by ClustalX program, and the dendrogram was constructed by the neighbor-joining method of clustering based on a PAM Matrix. Bootstrap values were computed over 1,000 replications. The Genbank accession numbers are as follows: <i>Bombyx mori</i>, XP_004922005.1; <i>Bombyx mori</i>, XP_004922004.1; <i>Plutella xylostella</i>, AFI55112.1; <i>Spodoptera exigua</i>, AFM38213.1; <i>Tribolium castaneum</i>, NP_001036035.1; <i>Nasonia vitripennis</i>, XP_001604515.2; <i>Bombus terrestris</i>, XP_003400489.1;<i>Camponotus floridanus</i>, EFN67257.1; <i>Haemaphysalis longicornis</i>, BAC06447.1;<i>Drosophila melanogaster</i>, NP_647768.3; <i>Metaseiulus occidentalis</i>, XP_003741167.1;Aplysia californica, XP_005104423.1; <i>Aplysia californica</i>, XP_005104424.1;<i>Crassostrea gigas</i>, EKC38805.1; <i>Homo sapiens</i>, AAX54833.1; <i>Crassostrea gigas</i>, EKC38802.1; <i>Crassostrea gigas</i>, EKC38804.1; <i>Culex quinquefasciatus</i>, XP_001867701.1; <i>Monochamus alternates</i>, BAG13449.1; <i>Aedes aegypti</i>, AAB81850.1; <i>Harpegnathos saltator</i>, EFN79784.1; <i>Acromyrmex echinatior</i>, EGI58538.1.</p

Gene expression in different developmental stages of Fujian Oyster (1: Eggs; 2: Gastrula; 3: Trochophore; 4: D-larvae; 5: Umbo larvae; 6: eye spot larvae; 7∶0.5 h after settlement; 8∶6 h after attachment; 9∶9 h after attachment; 10∶24 h after attachment).

<p>Gene expression in different developmental stages of Fujian Oyster (1: Eggs; 2: Gastrula; 3: Trochophore; 4: D-larvae; 5: Umbo larvae; 6: eye spot larvae; 7∶0.5 h after settlement; 8∶6 h after attachment; 9∶9 h after attachment; 10∶24 h after attachment).</p

<i>Ca</i>-Chit mRNA expression profiles in the larvae from trochophore to umbo-veliger larvae by qRT-PCR (α) and whole mount in situ hybridization (β).

<p>Each bar represents the mean ± SD of three replicates from three oysters. Data with significant difference between each other at <i>P<0</i>.<i>05</i> are indicated by different letters. 1, A: trochophore no detectable signal; 2, B: D-veliger larvae signals in the visceral mass (arrow); 3, C, D and E: early stages of umbo-veliger signals in the visceral mass (arrow); 4, F: middle stage of umbo-veliger signals in the visceral mass (arrow); 5, G: later stage of umbo-veliger signals in the visceral mass (arrow); u: larval umbo; v: larval veliger. The scale of black bar is 50 um.</p

Summary statistics of the ESTs generated from the Fujian oyster through pyrosequencing.

<p>Summary statistics of the ESTs generated from the Fujian oyster through pyrosequencing.</p

Distribution of the lengths of the ESTs for Unigenes of Fujian Oyster by 454 sequencing.

<p>Distribution of the lengths of the ESTs for Unigenes of Fujian Oyster by 454 sequencing.</p

Most commonly expressed sequences with associated Blast matches of <i>C. angulata.</i>

<p>Most commonly expressed sequences with associated Blast matches of <i>C. angulata.</i></p