Total Synthesis of Dictyodendrins B and E

The concise synthesis of the novel telomerase inhibitors dictyodendrins B and E was completed in only 9 and 11 steps (longest linear sequence). The highly convergent strategy employed a palladium-catalyzed Larock indole synthesis and a palladium-mediated one-pot consecutive Buchwald–Hartwig amination/C–H activation reaction as key steps. The present synthesis exhibits respectable levels of atom-, redox-, and step-economy

Total Synthesis of Dictyodendrins B and E

The concise synthesis of the novel telomerase inhibitors dictyodendrins B and E was completed in only 9 and 11 steps (longest linear sequence). The highly convergent strategy employed a palladium-catalyzed Larock indole synthesis and a palladium-mediated one-pot consecutive Buchwald–Hartwig amination/C–H activation reaction as key steps. The present synthesis exhibits respectable levels of atom-, redox-, and step-economy

Total Synthesis of Dictyodendrins B and E

The concise synthesis of the novel telomerase inhibitors dictyodendrins B and E was completed in only 9 and 11 steps (longest linear sequence). The highly convergent strategy employed a palladium-catalyzed Larock indole synthesis and a palladium-mediated one-pot consecutive Buchwald–Hartwig amination/C–H activation reaction as key steps. The present synthesis exhibits respectable levels of atom-, redox-, and step-economy

The tobacco seeds of MSYY85 pelleted with T1 method were attracted by a small magnet.

<p><b>Ck</b>: the control seeds pelleted without magnetic powder and fluorescent materials did not be attracted by a magnet; <b>a</b>: the seeds pelleted with T1 (2 g seeds pelleted with 15 g bentonite and 84 g blend powder which consisted of 79.6 g talc, 0.2 g fluorescein and 4.2 g magnetic powder) were attracted by a magnet; the white arrow showed a small magnet.</p

Fluorescence in seedling of MSYY85 pelleted with T3 method under illumination of different lights.

<p><b>Ck</b>: the top row, seedlings from control seeds pelleted without rhodamine B and magnetic powder after 7 days germination (×7); two columns from left to right: under natural light and green light, respectively; <b>A</b> and <b>B</b>: seedlings from seeds pelleted with T3 (2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels)) germinated for 7 days (A, the second row) and 16 days (B, the third row), respectively (×7); <b>a</b>: the cotyledon of control seedling under green light excitation (546 nm) (×20); <b>b</b> and <b>c</b>: the cotyledon of T3 seedling under green light excitation (546 nm) after 7 and 16 days germination, respectively (×20).</p

Effects of dual-labeling methods on superoxidase dismutase (SOD) activity and malondialdehyde (MDA) content of tobacco seedling.

<p>*significant difference (α = 0.05, LSD) among treatments within the same variety. Error bars represent ±S.E.</p

Fluorescence in cracked seeds of MS YY85 pelleted with dual-labeling method under illumination of different lights.

<p>Ck: the top row, the control pellets treated without fluorescent materials and magnetic powder (×7); three columns from left to right: under natural light, blue light and green light, respectively; T: Seeds pelleted with fluorescent materials and magnetic powder under natural light; T1: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.6 g talc, 0.2 g fluorescein and 4.2 g magnetic powder (FR and MP dual-labels); T3: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels).</p

Fluorescence in seedling of MSYY85 pelleted with T1 method under illumination of different lights.

<p><b>Ck</b>: the top row, seedlings from control seeds pelleted without fluorescein and magnetic powder after 7 days germination (×7); two columns from left to right: under natural light and blue light, respectively; <b>A</b> and <b>B</b>: seedlings from seeds pelleted with T1 (2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.6 g talc, 0.2 g fluorescein and 4.2 g magnetic powder (FR and MP dual-labels)) germinated for 7 days (A, the second row) and 16 days (B, the third row), respectively (×7); <b>a</b>: the cotyledon of control seedling under blue light excitation (495 nm) after 7 days germination (×20); <b>b</b> and <b>c</b>: the cotyledon of T1 seedling under blue light excitation (495 nm) after 7 and 16 days germination, respectively (×20).</p

Effects of dual-labeling methods on chlorophyll a (Chl-a), chlorophyll b (Chl-b), total chlorophyll content (Chl a+b) and carotenoids (Cx-c) contents in tobacco seedling.

<p>*significant difference (α = 0.05, LSD) among treatments within the same variety. Error bars represent ±S.E.</p

Additional file 1 of SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53

Supplementary Material