Pathology analysis of the mice injected with Ba/F3 cells, control p185 cells and p185 cells transduced with Abi1 shRNA

() Spleen weight of mice injected with Ba/F3 cells (control) and the p185 cells expressing with (p185 + Abi1 shRNA) or without (p185) Abi1 shRNA. () Histology of spleens and livers from the mice receiving Ba/F3 cells (control) and the p185 cells expressing with (p185 + Abi1 shRNA) or without (p185 ctrl) Abi1 shRNA. Arrowheads indicate the massively expanded p185 cells.<p><b>Copyright information:</b></p><p>Taken from "Abi1 gene silencing by short hairpin RNA impairs Bcr-Abl-induced cell adhesion and migration and leukemogenesis "</p><p></p><p>Carcinogenesis 2008;29(9):1717-1724.</p><p>Published online 2 May 2008</p><p>PMCID:PMC2527646.</p><p></p

Distribution of the intra-specific and inter-specific genetic variabilities (K2P distance) of cytochrome c oxidase I.

<p>Distribution of the intra-specific and inter-specific genetic variabilities (K2P distance) of cytochrome c oxidase I.</p

List of the morphological characters of Polytremis spp.

<p>List of the morphological characters of Polytremis spp.</p

The ML tree and networks for COI. (A) Maximum-likelihood phylogeny on the basis of the mitochondrial COI sequences.

<p>A 50% majority-rule consensus bootstrap tree is shown, with bootstrap values over 50% at the nodes. (B) Network on the basis of the mitochondrial COI sequences constructed with software NETWORK4.5. In the figures, yellow dots indicate the haplotypes from Genus <i>Polytremis</i> and red dots indicate the haplotypes from outgroups. The sequences are labelled according to the numbers shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084098#pone-0084098-t001" target="_blank">Table 1</a>.</p

List of taxa and voucher specimens used for sequencing.

<p>indicates the sequences retrieved from GenBank.</p

Abi1 knockdown inhibited Bcr-Abl-stimulated actin cytoskeleton remodeling, MT1-MMP clustering, as well as cell adhesion and migration on fibronectin-coated surfaces

() Inhibition of Bcr-Abl-induced abnormal actin remodeling by Abi1 knockdown. Ba/F3 cells and the Ba/F3 cells expressing p185, p185 and p185 plus Abi1 shRNA were fixed and stained with TRITC-conjugated phalloidin. The cells with F-actin-rich structures (invadopodia-like structures) were visualized by fluorescence microscopy as shown by arrowheads (upper panel) and were counted (lower panel, represented as mean ± SD percentage of three randomly picked areas). () The p185 cells expressing GFP–MT1-MMP were transduced with either control retrovirus (control) or the retrovirus expressing Abi1 shRNA. The knock down of Abi1 expression was confirmed by western blotting (data not shown). The distribution of GFP–MT1-MMP was visualized by fluorescence microscopy. A similar result has been described previously (). () Effects of Abi1 knockdown on Bcr-Abl-stimulated cell adhesion (lower panel) and migration (upper panel) on fibronectin-coated surfaces. Ba/F3 cells and the p185 cells transduced with either non-silencing shRNA or Abi1 shRNAs were grown in fibronectin-coated six-well plate (2.5 × 10 per well) for 16 h. The total cells and the cells that were adherent to fibronectin-coated surfaces were counted and the percentage of adherent cells calculated. The vertical axis shows the percentage of the adherent cells and is expressed as the mean ± SD of triplicate wells. For cell migration on fibronectin-coated membrane, 1 × 10 cells were tested in Transwell migration assay. The vertical axis shows the percentage of the migrated cells and is expressed as the mean ± SD of triplicate wells; * < 0.01.<p><b>Copyright information:</b></p><p>Taken from "Abi1 gene silencing by short hairpin RNA impairs Bcr-Abl-induced cell adhesion and migration and leukemogenesis "</p><p></p><p>Carcinogenesis 2008;29(9):1717-1724.</p><p>Published online 2 May 2008</p><p>PMCID:PMC2527646.</p><p></p

Maximum-likelihood phylogeny on the basis of the concatenated sequences (mitochondrial COI+nuclear rDNA) haplotypes.

<p>A 50% majority-rule consensus bootstrap tree is shown.</p

Transitions and transversions plotted against Kimura-2-parameter distance.

<p>(A) the mtDNA cytochrome <i>c</i> oxidase I (COI) fragment in <i>Polytremis</i>; (B) the concatenated sequences (mt DNA COI+nuclear rDNA).</p

The knock down of Abi1 expression attenuated Bcr-Abl-induced activation of Lyn kinases

Ba/F3 cells (lanes 1 and 4), control p185 cells (lanes 2 and 5) and p185 Abi1 shRNA cells (lanes 3 and 6) were starved in RPMI 1640 plus 0.1% bovine serum albumin for 6 h. The cells were then treated without (lanes 1–3) or with (lanes 4–6) recombinant rat IL3 (20 ng/ml) for 10 min. The lysates (2 × 10 cells) from indicated cell lines were immunoprecipitated (IP) with anti-Lyn antibodies, separated on 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and subjected to western blot (WB) analysis using anti-Src p416 antibody, which recognizes activated Lyn (upper panel). The membrane then was stripped and reprobed with anti-Lyn antibodies (lower panel). Two Lyn isoforms, p53Lyn and p56Lyn, were indicated.<p><b>Copyright information:</b></p><p>Taken from "Abi1 gene silencing by short hairpin RNA impairs Bcr-Abl-induced cell adhesion and migration and leukemogenesis "</p><p></p><p>Carcinogenesis 2008;29(9):1717-1724.</p><p>Published online 2 May 2008</p><p>PMCID:PMC2527646.</p><p></p

Support tree for the relationships within the genus <i>Polytremis</i> based on a combined analysis.

<p>Support tree based on 20 morphological characters and 4 genes. Supports for nodes are given in bootstrap values.</p