Additional file 1 of Temporal topic model for clinical pathway mining from electronic medical records

Additional file 1

Primers for energy metabolism genes.

<p>Primers for energy metabolism genes.</p

Primers for Bacteroidetes, Firmicutes, and total bacteria.

<p>Primers for Bacteroidetes, Firmicutes, and total bacteria.</p

Effects of RC and berberine on gene expressions in visceral adipose tissues and livers.

<p>“Normal”, normal chow diet-fed mice; “HFD”, high-fat diet-fed control mice; “berberine”, berberine-treated HFD mice; “RC”, <i>Rhizoma coptidis</i>-treated HFD mice. Data were expressed as Mean±S.D. (n = 6), *<i>P</i><0.05, **<i>P</i><0.01 vs. HFD controls.</p

Effects of RC and berberine on growth of fecal bacteria ex vivo under (a) aerobic and (b) anaerobic conditions.

<p>“Normal”, normal chow diet-fed mice; “HFD”, high-fat diet-fed control mice; “Berberine”, Berberine-treated HFD mice; “RC”, Rhizoma Coptidis-treated HFD mice (n = 6).</p

Effects of RC and berberine on Fiaf protein expressions in intestinal and adipocyte tissues.

<p>“Normal”, normal chow diet-fed mice; “HFD”, high-fat diet-fed control mice; “berberine”, berberine-treated HFD mice; “RC”, <i>Rhizoma coptidis</i>-treated HFD mice. Data were expressed as Mean±S.D. (n = 6), *<i>P</i><0.05, **<i>P</i><0.01 vs. HFD controls.</p

Effects of (a) berberine and (b) RC on the growth of <i>Lactobacillus</i> sp. under anaerobic conditions in vitro.

<p>Effects of (a) berberine and (b) RC on the growth of <i>Lactobacillus</i> sp. under anaerobic conditions in vitro.</p

Effects of RC and berberine on (a) body weight and (b) visceral adipose tissue weight in HFD mice.

<p>“Normal”, normal chow diet-fed mice; “HFD”, high-fat diet-fed control mice; “berberine”, berberine-treated HFD mice; “RC”, <i>Rhizoma coptidis</i>-treated HFD mice. Data were expressed as Mean±S.D. (n = 10), *<i>P</i><0.05, **<i>P</i><0.01 vs. HFD controls.</p

Effects of RC and berberine on blood biochemical assay parameters, metabolic cage trials, and fecal lipid and polysaccharide evaluations in HFD mice.

<p>“Normal”, normal chow diet-fed mice; “HFD”, high-fat diet-fed control mice; “berberine”, berberine-treated HFD mice; “RC”, <i>Rhizoma coptidis</i>-treated HFD mice. Data were expressed as Mean±S.D. (n = 10),</p><p>*<i>P</i><0.05,</p><p>**<i>P</i><0.01 vs. HFD controls.</p

HPLC fingerprint of ethanol extracts of <i>Rhizoma coptidis</i> (RC).

<p>Chromatogram conditions: Kromasil C18 column (5 µm, 4.6 mm×250 mm), column temperature, 30°C; mobile phase, acetonitrile: H₂O (0.05 M KH2PO4, pH 3.0) = 30∶70 (v/v); elution rate, 1 ml/min; detector wavelength, 345 nm. Peak “1”, berberine (content, 24.2%).</p