12 research outputs found
Diversification of Monoterpene Indole Alkaloid Analogs through Cross-Coupling
<i>Catharanthus roseus</i> monoterpene indole alkaloid analogs have been produced via a combination of biosynthetic and chemical strategies. Specifically, introduction of a chemical handle顥竌 chlorine or a bromine顥竔nto the target molecule by mutasynthesis, followed by postbiosynthetic chemical derivatization using Pd-catalyzed Suzuki-Miyaura cross-coupling reactions robustly afforded aryl and heteroaryl analogs of these alkaloids
MOESM1 of Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate
Additional file 1. Supplementary information for metabolic engineering of the methylotrophic yeast pichia pastoris for production of isobutanol and isobutyl acetate
Functional annotation results.
<p>Proportion of <i>Camptotheca acuminata</i>, <i>Catharanthus roseus</i> and <i>Rauvolfia serpentina</i> transcripts with sequence similarity to the UniRef100 database <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052506#pone.0052506-Suzek1" target="_blank">[17]</a>, <i>Arabidopsis thaliana</i> proteome (<a href="http://arabidopsis.org" target="_blank">http://arabidopsis.org</a>), and Pfam domain database <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052506#pone.0052506-Punta1" target="_blank">[18]</a>.</p
Monoterpene indole alkaloid pathway.
<p>The key intermediate strictosidine is formed by condensation of tryptamine, which contributes the indole ring, and secologanin, which is produced from the monoterpene geraniol. In various plants, strictosidine is further metabolized to generate over 2,500 monoterpene indole alkaloids. Solid lines indicate single enzymatic steps; dashed lines indicate multiple steps.</p
Phylogenetic relationships.
<p><i>Camptotheca acuminata</i> (Nyssaceae) is in the order Cornales within the asterid superorder of core eudicots, and <i>Catharanthus roseus</i> and <i>Rauvolfia serpentina</i> (both Apocynaceae) are in Gentainales, also within the asterids. <i>Arabidopsis thaliana</i> is in the family Brassicales within the rosid superorder. Fabales and Astrales are shown for orientation. Redrawn and greatly simplified from APG III <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052506#pone.0052506-APGIII1" target="_blank">[19]</a>.</p
Libraries and sequencing generated for <i>Camptotheca acuminata</i>.
1<p>Libraries used in the first, initial de novo assembly.</p>2<p>Libraries only sequenced for expression abundance estimates, reads were not included in de novo assembly.</p>3<p>Libraries used in the second, final de novo assembly.</p><p>M: Million.</p
Cluster of orthologous and paralogous genes families in <i>Camptotheca acuminata</i>, <i>Catharanthus roseus,</i> and <i>Rauvolfia serpentina</i> species as identified by OrthoMCL.
<p>Predicted peptides from the <i>Camptotheca acuminata</i>, <i>Catharanthus roseus</i> and <i>Rauvolfia serpentina</i> transcriptomes were clustered using OrthoMCL <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052506#pone.0052506-Li1" target="_blank">[23]</a>. A) Number of clusters (c) and genes (g) for each orthologous group. B) Number of genes in the different clusters for each species. The number of clusters and genes for each OrthoMCL group are shown. Group 1: Clusters (blue) and genes shared among <i>C. acuminata</i> (red), <i>C. roseus</i> (green) and <i>R. serpentina</i> (purple). Group 2: Clusters (blue) and genes shared among <i>C. acuminata</i> (red) and <i>C. roseus</i> (green). Group 3: Clusters (blue) and genes shared among <i>C. roseus</i> (green) and <i>R. serpentina</i> (purple). Group 4: Clusters (blue) and genes shared among <i>C. acuminata</i> (red) and <i>R. serpentina</i> (purple).</p
Hierarchical clustering of expression profiles from sampled tissues from <i>Camptotheca acuminata</i> (A), <i>Catharanthus roseus</i> (B), and <i>Rauvolfia serpentina</i> (C).
<p>Pearson product-moment correlation coefficient of log<sub>2</sub> FPKM (fragments per kb transcript per million mapped reads) expression values among RNA-seq libraries were calculated and clustered using R <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052506#pone.0052506-R1" target="_blank">[39]</a>; negative values were set to zero.</p
Summary of statistics of the transcriptome de novo assemblies of <i>Camptotheca acuminata</i>, <i>Catharanthus roseus</i>, and <i>Rauvolfia serpentina</i>.
<p>Summary of statistics of the transcriptome de novo assemblies of <i>Camptotheca acuminata</i>, <i>Catharanthus roseus</i>, and <i>Rauvolfia serpentina</i>.</p