The role of macrophages in the non-specific stimulation of the antibody response as studied in vitro

Imperial Users onl

“I wish I’d told them”: a qualitative study examining the unmet psychosexual needs of prostate cancer patients during follow-up after treatment

<b>Objective</b> To gain insight into patients' experiences of follow-up care after treatment for prostate cancer and identify unmet psychosexual needs.<p></p> <b>Methods</b> Semi-structured interviews were conducted with a purposive sample of 35 patients aged 59-82 from three UK regions. Partners were included in 18 interviews. Data were analyzed using constant comparison. <p></p> <b>Results</b> (1) Psychosexual problems gained importance over time, (2) men felt they were rarely invited to discuss psychosexual side effects within follow-up appointments and lack of rapport with health care professionals made it difficult to raise problems themselves, (3) problems were sometimes concealed or accepted and professionals' attempts to explore potential difficulties were resisted by some, and (4) older patients were too embarrassed to raise psychosexual concerns as they felt they would be considered 'too old' to be worried about the loss of sexual function.<p></p> <b>Conclusion</b> Men with prostate cancer, even the very elderly, have psychosexual issues for variable times after diagnosis. These are not currently always addressed at the appropriate time for the patient.Practice implications Assessments of psychosexual problems should take place throughout the follow-up period, and not only at the time of initial treatment. Further research examining greater willingness or reluctance to engage with psychosexual interventions may be particularly helpful in designing future intervention

Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins.

Na/K-ATPase plays an important role in mediating blastocyst formation. Despite the expression of multiple Na/K-ATPase alpha and beta isoforms during mouse preimplantation development, only the alpha1 and beta1 isoforms have been localized to the basolateral membrane regions of the trophectoderm. The aim of the present study was to selectively down-regulate the Na/K-ATPase beta1 subunit employing microinjection of mouse 1 cell zygotes with small interfering RNA (siRNA) oligos. Experiments comprised of non-injected controls and two groups microinjected with either Stealthtrade mark Na/K-ATPase beta1 subunit oligos or nonspecific Stealthtrade mark siRNA as control. Development to the 2-, 4-, 8-, and 16-cell and morula stages did not vary between the three groups. However, only 2.3% of the embryos microinjected with Na/K-ATPase beta1 subunit siRNA oligos developed to the blastocyst stage as compared with 73% for control-injected and 91% for non-injected controls. Na/K-ATPase beta1 subunit down-regulation was validated by employing reverse transcription-PCR and whole-mount immunofluorescence methods to demonstrate that Na/K-ATPase beta1 subunit mRNAs and protein were not detectable in beta1 subunit siRNA-microinjected embryos. Aggregation chimera experiments between beta1 subunit siRNA-microinjected embryos and controls demonstrated that blockade of blastocyst formation was reversible. The distribution of Na/K-ATPase alpha1 and tight junction-associated proteins occludin and ZO-1 were compared among the three treatment groups. No differences in protein distribution were observed between control groups; however, all three polypeptides displayed an aberrant distribution in Na/K-ATPase beta1 subunit siRNA-microinjected embryos. Our results demonstrate that the beta1 subunit of the Na/K-ATPase is required for blastocyst formation and that this subunit is also required to maintain a normal Na/K-ATPase distribution and localization of tight junction-associated polypeptides during preimplantation development

Platform urbanism, smartphone applications and valuing data in a smart city

Recent scholarship on smart cities and platform urbanism has explored the very wide range of data harvested from urban environments by digital devices of many kinds, analysing how not only efficiencies but also profits are sought through the extraction, circulation, transformation, commodification, integration, and re‐use of data. Much of that data is generated by smartphone applications. This paper looks at the design of a group of eight smartphone apps by a range of different actors in Milton Keynes, a small UK city with a large number of smart city initiatives. The apps are understood as a co‐constitutive interface between data circulations and embodied users. The paper focuses specifically on the data that the apps generated and shared and on how the app designers anticipated that the data would create different kinds of value for embodied app users. While some data circulations were understood as ways of generating financial value, the paper argues that a number of other forms of value were assumed in the app design. The paper identifies two of these, which it terms normative values and interactive values. It examines how the data mobilised by the smart city apps enacts particular versions of these values, and how those values co‐constitute specific kinds of bodies, agencies, and geographies in digitally mediated cities

More-than-Human Wellbeing: Materialising the Relations, Affects, and Agencies of Health, Kinship, and Care

Introduction The concept of ‘wellbeing’ is typically thought of in human-centric ways, referring to the affective feelings and bodily sensations that people may have which inform their sense of health, safety, and connection. However, as our everyday lives, identities, relationships, and embodiments become digitised and datafied, ‘wellbeing’ has taken on new practices and meanings. The use of digital technologies such as mobile and wearable devices, social media platforms, and networks of information mediate our interactions with others, as well as the ways we conceptualise what it means to be human, including where the body begins and ends. In turn, digital health technologies and ‘wellness’ cultures such as those promoted on social media sites such as Instagram, Pinterest, YouTube, TikTok, and Facebook have also shaped our understanding of ‘wellness’ and ‘wellbeing’, their parameters, and how they ought to be practiced and felt (Baker; Lupton Digital Health; Lupton et al.). For millennia, aspects of human bodies have been documented and materialised in a variety of ways to help people understand states of health and illness: including relationships to the environments in which they lived. Indigenous and other non-Western cosmologies have long emphasised the kinds of vibrancies and distributed agencies that are part of reciprocal more-than-human ‘manifestings’ of kinships, and have called for all people to adopt the role of stewards of the ecosystem (Bawaka Country et al.; Hernández et al.; Kimmerer; Rots; Todd; Tynan). In Western cultures, ideas of the human body that reach back to ancient times adopt a perspective that viewed the continuous flows of forces (the four humours) in conjunction with the elements of air, wind, earth, and fire inside and outside the body as contributing to states of health or ill health. It was believed that good health was maintained by ensuring a balance between these factors, including acknowledgement of the role played by climactic, ecological, and celestial conditions (Hartnell; Lagay). A more-than-human approach is beginning to be re-introduced into Western cultures through political activism and academic thinking about the harms to the planet caused by human actions, including global warming and climate crises, loss of habitats and ecological biodiversity, increased incidence of extreme weather events such as bushfires, floods, and cyclones, and emerging novel pathogens affecting the health not only of humans but of other living things (Lewis; Lupton Covid Societies; Lupton Internet of Animals; Neimanis et al.). Contemporary Western more-than-human philosophers argue for the importance of acknowledging our kinship with other living and non-living things as a way of repositioning ourselves within the cosmos and working towards better health and wellbeing for the planet (Abram; Braidotti; Plumwood). As these approaches emphasise, health, wellbeing, and kinship are always imbricated within material-social assemblages of humans and non-digital things which are constantly changing, and thereby generating emergent rather than fixed capacities (Lupton "Human-Centric"; Lupton et al.). In this article, we describe our More-than-Human Wellbeing exhibition. To date, new media, Internet, and communication studies have not devoted as much attention to more-than-human theory. It is this more-than-digital and more-than-human approach to health information and wellbeing that marks out our research program as particularly distinctive. Our research focusses on the many and varied digital and non-digital forms that information about health and bodies takes. We are interested in health data as they are made and form part of the objects and activities of people’s everyday lives and aim to expand the human-centric approach offered in digital health by positioning human health and embodiment as always imbricated within more-than-human ecosystems. We acknowledge that all environments (natural and human-built) are intertwined with humans, and that to a greater or lesser extent, all are configured with and through the often exploitative and extractive practices and ideologies of those living in late modern societies in which people are positioned as superior to and autonomous from other living things. Together with more-than-human scholarship, we take inspiration from work in which arts-based, multisensory, and museum curation methods are employed to draw attention to the intertwining of people and ecologies (Endt-Jones; Howes). Our exhibition was planned as a research translation and engagement project, communicating several of our studies’ findings in arts-based media (Lupton "Embodying"). In what follows, we outline the concepts leading to the creation of our exhibits and describe how these pieces materialise and extend more-than-human concepts of wellbeing and care. Five of the exhibits we created for this exhibition are discussed. They all draw on our research findings across a range of studies, together with more-than-human theory and medical history (Lupton "More-Than-Human"). We describe how we used these pieces to materialise more-than-human concepts of health, wellbeing, and kinship in ways that we hoped would provoke critical thought, affective responses, and open capacities for action for contributing to both human and nonhuman flourishing. The background, thinking, and modes of making leading to the creation of ‘Cabinet of Human/Digital/Data Curiosities’, ‘Smartphone Fungi’, ‘Hand of Signs’, ‘Silken Anatomies’, and ‘Talking/Flowers’ are explained below. Bodily Curios Vaughan Wozniak-O’Connor and Deborah Lupton. Cabinet of Human/Digital/Data Curiosities. Reclaimed timber, found objects, resin 3D prints. 2023. Fig. 1: Cabinet of Human/Digital/Data Curiosities. Fig. 2: Detail from Cabinet of Human/Digital/Data Curiosities. The objects we have placed in Cabinet of Human/Digital/Data Curiosities (figs. 1 and 2) mix together such things from the past as prosthetic human eyeballs and teeth used in medicine and dentistry in earlier eras. This collection of found and manufactured objects, both old and new, draws on the concept of the ‘cabinet of curiosities’, also known as cabinets of wonder, which first became popular in the sixteenth century. Artefacts were assembled together for viewing in a room or a display case. The items were chosen for being notable in some way by the curator, including objects from natural history, antiquities, and religious relics, as well as works of art. These collections, purchased, curated, and assembled by members of the nobility or the wealthy as a marker of refinement, knowledge, or social status, were the precursor of museums (Endt-Jones). We see digital devices such as mobile phones as one of a multitude of ways that operate to document and preserve elements of human embodiment – indeed, as contemporary ‘cabinets of curiosities’. Our cabinet also refers to the tradition of medical museums, which display preserved human organs, body parts, and tissue in glass bottles for pedagogical purposes. Under this model of health, specimens of both ‘ideal’ health and also ‘ill’ health – abnormalities in the flesh – were documented as a means of categorising wellbeing. Museums such as these would often treat diseased and disabled bodies as oddities and artefacts of ‘curiosity’. In this work, we reimagine and wind back this way of thinking, through displaying and drawing attention and curiosity towards signs of the body and the everyday. We are showing that wellbeing is more than a process of categorisation, comparison, or measurement of ‘ideal’ or ‘abnormal’; it is in the traces we leave behind us when we return to the earth. Our information data are human remains, moving as endless constellations of the interior and exterior of the body (Lupton Data Selves). In this artwork, both reclaimed wood and 3D-printed resin were used as a synergy between the natural and synthetic. Taking our cue from the manner of display of these items in medical museums, we have added our own curios, including 3D-printed body organs sprouting fungi (fig. 2), as a way of demonstrating the entanglements between humans and the fungal kingdom. Interspersed among these relics of human bodies is a discarded mobile phone with its screen badly shattered. It is displayed as a more recent antiquated object for making images and collecting, storing, and displaying information and images about human bodies, which itself is subject to disastrous events despite its original high-tech veneer of glossy impermeability. Technologies are more-than-flesh as human-made simulacra of body parts. Our wellbeing is sensed and made sense of through bodies’ entanglements of human and nonhuman. These curios both materialise traces of our bodies and wellbeing and extend our bodies into the physical spaces we inhabit and through which we move. Reading the Traces and Signs Vaughan Wozniak-O’Connor and Deborah Lupton. Smartphone Fungi. Recycled European oak, 3D printed resin, CNC carved plywood. 2023 Vaughan Wozniak-O’Connor and Deborah Lupton. Hand of Signs. Laser-etched walnut and plywood. 2023. Fig. 3: Smartphone Fungi. / Fig. 4: Detail from Smartphone Fungi. Wellbeing is also a process of mark-making, realised through the reciprocal impressions we leave on each other and the world around us. In Smart Phone Fungi (figs. 3 and 4) we capture the idea of ‘recording’ that takes place between people, technologies, and the natural world. It was inspired by a huge tree which members of our team noticed on a bush walk in the Blue Mountains, near Sydney, Australia. Growing from this tree were fungi of similar size and shape to the smartphone that was used to capture the image. In our interpretation, a piece of reclaimed timber was used to represent the tree, itself marked by its human use, and fungal shapes replicating those on the tree were produced using computer numerical controlled (CNC) carving. The central timber post is covered with human and more-than-human traces, such as old tool marks, weather damage, and wood borer holes. Alongside these traces, the CNC-carved fungi forms add a conspicuously digital layer of human intervention. Fig. 5: Hand of Signs. In Hand of Signs (fig. 5), we extend this idea of both organic and digital data traces as something that can be ‘read’ or interpreted. Inspired by the practice of palmistry, this work re-interprets line reading, the historical wooden anatomical model, and human body scanning as ways of reading for signs of wellbeing in past and future. Palm readers interpret people’s character, health, longevity, and other aspects of their lives through the creases and traces of development, wear, and deteriorations in the skin of our hands (Chinn). Life leaves its traces on our palms. The piece also refers to the newer tradition of digitising human bodies (Lupton Quantified Self; Lupton et al.), employing scanning and data visualising technologies, which uses spatial GPS data to deduce patterns of human activity. For both palmistry and in more contemporary monitoring technologies, one’s wellbeing can be deduced through the map: the lines of the palm and the errant traces collected by satellites and sensors. To reflect this relation between mapping and palmistry, our updated anatomical model references both the contours of 3D geospatial data and of the human palm. However, this piece looks to represent more layers of data beyond those captured by GPS data. By using reclaimed wood to construct this human hand model, we are again making an analogy between the marks of growth and life that timber displays and those that the human body bears and develops as people move through more-than-worlds throughout their lifespans. The piece also seeks to draw attention to the various ‘signs’ that have been used across centuries to interpret the current and future health and wellbeing of humans (once markings on or morphologies of the body, now often the digitised visualisations of the internal operations and physical movements of the body that are generated by digital health technologies), superimposing older and newer modes of corporeal knowledge. Layers of Mediation Megan Rose. Silken Anatomies. Digital print on satin and yoryu silk chiffon. 2023. Ash Watson. Talking/Flowers. Collage and digital inkjet on paper. 2023. Fig. 6: Detail from Silken Anatomies. The ways that we come to sense and understand wellbeing are also mediated through the reproductive interplay of natural and technological elements. Silken Anatomies (fig. 6) was inspired by anatomical prints from the Renaissance showing details of the interiors of human bodies and organs together with living things and objects from the natural world. These webs of interconnectivity were thought to be key to wellbeing and health. Produced at scale through metal engraving and woodblock printing, these natural history and compendia took on major importance as part of these educational resources (Kemp; Swan). In an effort to extend the reach of artefacts beyond their tangible presence, libraries globally have sought to create open access digital scans of historic medical and botanical illustrations. The images reconfigured in Silken Anatomies were downloaded from the Wellcome Trust’s online archive and have been reimagined through digital enhancement and sublimation dye techniques. Referencing shrouds, the yoryu silk panels enfold exhibition visitors, who were able to touch and pass through the silks, causing them to billow in response to human movement. We bring together an animal-made material (crafted by silkworms) with more-than-human images featuring both humans and other living creatures. The vibrancies of these beautifully engraved and coloured anatomical images are given a new life and a new feel, both affectively and sensuously, through this piece. We can both see and touch these more-than-human illustrations that speak to us of the early modern natural science visualisations that underpin contemporary digital images of the human body and the more-than-human world. The vibrancies of these beautifully engraved and coloured anatomical plates are given a new life and a new feel, both affectively and sensuously. The digital is returned to the tangible. Fig. 7: Detail from Talking/Flowers. Even in increasingly digitised healthcare environments, paper and other printed materials remain central documents in the landscape of health and wellbeing. Zines are small-scale, DIY, and typically handcrafted publications, which are often made to express creators’ thoughts and feelings about health and wellbeing (Lupton "Health Zines"; Watson and Bennett). Talking/Flowers (fig. 7), a zine of visual and textual work, explores the materialities of health information and healthcare encounters by creatively layering a diverse range of materials: clippings from MRI scans, digitally warped and recoloured images from medical infographics, and found poetry made from research publications. In this way, the zine remixes and reconstitutes key documents of authority in health institutions which continue to take primacy as evidence. While vital in the pipeline of diagnosis and treatment, such documents can become black boxes of meaning, and serve to distance health professionals from consumers and consumers from agentic understandings of their own health. These evidentiary materials are brought together here with other imagery, textures, and recollections of personal experience; the pages also feature leaves, flowers, fungi, and oceanic tones. Oceans, pools, rivers, lakes, and other coastal forms or waterways offer all-consuming sensory spaces in which people can find calm, balance, buoyancy, and connection with the wider world. Aqua tides, purple eddies, and misshapen pearls flow through the pages as the golden thread of the zine’s aesthetic theme. Also featured are three original poems. The first and third poems, ‘talking to a doctor’ and ‘talking to other people’, explore moments of relational vulnerability. The second poem, ‘untitled’, is a found poem made from the conclusions of sociologist Talcott Parsons’s 1975 article on the sick role reconsidered. In each of these poems, information and communication jar the encounters and more-than-human metaphors hold space for complex feelings. The cover similarly merges imagery from botanical and historical medical illustrations with a silver shell, evoking the morphological dimensions that connect the more-than-human. Exhibition visitors were able to turn the pages of the original copy of the zine, and were invited to take a printed copy away with them. Conclusion More-than-Human Wellbeing is an exhibition which aims to expand the horizons of how we understand wellbeing and our entanglements with the world. Our exhibition was designed to draw on our research into the more-than-human dimensions of health and wellbeing in the context of an increasingly digitised and datafied world. We wanted to attune visitors to the relational connections and multisensory ways of knowing that develop with and through people’s encounters and entanglements with creatures, things, and spaces. We sought to demonstrate that in this digital age, in which digital devices and software are often considered the most accurate and insightful ways to monitor and measure health and wellbeing, multisensory and affective engagements with elements of the natural environment remain crucial to understanding our bodies and health. Through engagements with our artworks, we hoped that new capacities for visitors’ learning and thinking about the relational and distributed dimensions of more-than-human wellbeing would be opened. While traditionally thought of as human-centered, we explore human health and wellbeing as interconnected with both the natural and technological. We used materials from the natural world – timber, paper materials, and silk fabric – in our artworks to capture both the multigenerational traces and entanglements between humans and plant matter. Recent works of natural and cultural history have drawn attention to the mysterious and important worlds of the fungi kingdom and its role in supporting and living symbiotically with other life on earth, including humans as well as plants (Sheldrake; Tsing). We also made sure to acknowledge this third kingdom of living things in our artworks. We combined these images and materials from nature with digitised modes of printing and fabrication to highlight the intersections of the digital with the non-digital in representations and sensory feelings of health and wellbeing. We disrupt and make strange signs of traditional human-centric medicine through reconfigurations, bricolage, and re-imaginations of more-than-human wellbeing. As humans we are interconnected with the natural world, and the signs of these meetings can be traced and read. Through our artistic creations, we hope to re-orient people towards this more open way of thinking about wellbeing. Working with arts practices and creative data visualisations, both digital and analogue, we bring to the fore the role that more-than-human agents play in mediating and making these convivial more-than-digital connections. Acknowledgments This research was funded by the ARC Centre of Excellence for Automated Decision-Making and Society (CE200100005) and a Faculty of Arts, Design & Architecture collaboration grant. UNSW Library provided financial and curatorial support for the mounting of the exhibition. References Abram, David. "Wild Ethics and Participatory Science: Thinking between the Body and the Breathing Earth." Planet. Volume 1. Kinship: Belonging in a World of Relations. Eds. Gavin van Horn et al. Center for Humans & Nature Press, 2021. 50-62. Baker, Stephanie Alice. Wellness Culture: How the Wellness Movement Has Been Used to Empower, Profit and Misinform. Emerald Group, 2022. Bawaka Country, et al. "Working with and Learning from Country: Decentring Human Author-Ity." cultural geographies 22.2 (2015): 269-283. DOI: 10.1177/1474474014539248. Braidotti, Rosi. "'We' Are in This Together, But We Are Not One and the Same." Journal of Bioethical Inquiry 17 (2020): 465-469. DOI: 10.1007/s11673-020-10017-8. Chinn, Sarah E. Technology and the Logic of American Racism: A Cultural History of the Body as Evidence. Continuum, 2000. Endt-Jones, Marion. "Cultivating ‘Response-Ability’: Curating Coral in Recent Exhibitions." Journal of Curatorial Studies 9 (2020): 182-205. DOI: 10.1386/jcs_00020_1. Hartnell, Jack. Medieval Bodies: Life, Death and Art in the Middle Ages. Profile Books, 2018. Hernández, K.J., et al. "The Creatures Collective: Manifestings." Environment and Planning E: Nature and Space 4.3 (2020): 838-863. DOI: 10.1177/2514848620938316. Howes, David. "Introduction to Sensory Museology." The Senses and Society 9.3 (2014): 259-267. DOI: 10.2752/174589314X14023847039917. Kemp, Martin. "Style and Non-Style in Anatomical Illustration: From Renaissance Humanism to Henry Gray." Journal of Anatomy 216.2 (2010): 192-208. DOI: 10.1111/j.1469-7580.2009.01181.x. Kimmerer, Robin. "Restoration and Reciprocity: The Contributions of Traditional Ecological Knowledge." Human Dimensions of Ecological Restoration: Integrating Science, Nature, and Culture. Eds. Dave Egan et al. Springer, 2011. 257-276. Lagay, Faith. "The Legacy of Humoral Medicine." AMA Journal of Ethics 4.7 (2002): 206-208. Lewis, Bradley. "Planetary Health Humanities—Responding to Covid Times." Journal of Medical Humanities 42.1 (2021): 3-16. DOI: 10.1007/s10912-020-09670-2. Lupton, Deborah. Covid Societies: Theorising the Coronavirus Crisis. Routledge, 2022. ———. Data Selves: More-than-Human Perspectives. Polity Press, 2019. ———. Digital Health: Critical and Cross-Disciplinary Perspectives. Routledge,

A comparison of soil liming requirement methodologies in temperate, Northern European pedo‐climates

ACKNOWLEDGEMENTS Funding for this research was provided through a PhD studentship by Scotland's Rural College (SRUC) and the University of Aberdeen.Peer reviewedPublisher PD

Initiating e-learning by stealth, participation and consultation in a late majority institution

The extent to which opportunities afforded by e-learning are embraced by an institution can depend in large measure on whether it is perceived as enabling and transformative or as a major and disruptive distraction. Most case studies focus on the former. This paper describes how e-learning was introduced into the latter environment. The sensitivity of competing pressures in a research intensive university substantially influenced the manner in which e-learning was promoted. This paper tells that story, from initial stealth to eventual university acknowledgement of the relevance of e-learning specifically to its own context

A preliminary investigation of the effect of ethical labeling and moral self-image on the expected and perceived flavor and aroma of beer

Ethical labelling has been shown to influence taste/flavour perception. Across two experiments, the present study examined how ethical labelling and moral self-image influenced both the expected (Experiment One) and perceived (Experiment Two) taste/flavour characteristics of beer. In Experiment One, 170 participants read either a ‘moral’ or ‘control’ label describing a brewery, after which they were presented with an image of a beer. Participants then completed a Beer Taste Perception Questionnaire and the Moral Self-Image Scale. In Experiment Two, 59 participants were exposed to either the moral or control label before tasting a beer and completing the same questionnaires from Experiment One. The results of Experiment One indicated that label type moderated the relationship between moral self-image and the intensity ratings of the beer. Specifically, in the presence of a control label, the expected intensity of the beer’s flavour increased as moral self-image increased. Experiment Two found no evidence that the moral label influenced the perceived taste of the beer. However, the results showed that as moral self-image became more positive the perceived refreshingness of the beer increased. This study provides novel evidence of the potential relationship between an individual’s moral self-image and the expected and perceived taste/flavour characteristics of beer. © 2021 by the authors. Licensee MDPI, Basel, Switzerland