Low uric acid levels in patients with Parkinson's disease: evidence from meta-analysis

Figure S5. Transcript levels of (A) 18S rRNA, (B) GAPDH, and (C) β-actin mRNAs in samples fixed with ethanol for 10 s, 30 s, and 60 s in comparison with the untreated samples. Values are expressed as copy number of transcripts per 182 nm2 × 20 μm cryosection thickness. Bars indicate mean ± SEM. One-way ANOVA followed by Tukey post hoc test, where **p < 0.01, ***p < 0.001. n = 7 for the untreated samples, n = 6 for 10 s fixation, and n = 8 for 30 s and 60 s fixation

Additional file: 1 of Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis

Figure S1. Visualization of neurons of ethanol-fixed and NeuroTrace-stained third ventricle (D3V) specimen under (A1) a bright field and (A2) a fluorescence radiated field. The staining of the choroid plexus is thought to be a non-specific signal commonly observed in fresh frozen samples stained with regular Nissl stains, such as Cresyl violet, and is often considered negligible as it is irrelevant to the cerebral parenchyma. (A3) Ethanol-fixed hippocampal CA1 region under a fluorescent light, left side with the NeuroTrace stain and right side without it. (B1) Ethanol-fixed and NeuroTrace-stained neurons of the hippocampal DG region (B2) before and (B3) after microdissection, indicated by red arrows. Scales bars: (A) 200 Îźm, (B1) 100 Îźm, and (B2, B3) 25 Îźm

Additional file: 3 of Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis

Figure S3. (A–C) Correlation of the transcript levels between the housekeeping genes in unfixed and unstained (Untreated; crosses) and ethanol and NeuroTrace-treated (EtOH/NT; circles) samples: (A) β-actin vs. 18S rRNA, (B) GAPDH vs. 18S rRNA, and (C) GAPDH vs. β-actin. (D–E) Correlation between the transcript levels of the housekeeping genes and Map2: (D) β-actin vs. Map2, (E) 18S rRNA vs. Map2, and (F) GAPDH vs. Map2. Values are expressed as copy number of transcripts per LMD tissue of 182 nm2 × cryosection thickness in volume. For untreated samples, n = 7 for 20 μm, n = 6 for 30 μm, and n = 8 for 40 μm; for fixed and stained samples, n = 7 for 20 μm, n = 7 for 30 μm, and n = 8 for 40 μm