Identification of alternative splicing variants of the β subunit of human Ca2+/calmodulin-dependent protein kinase II with different activities

AbstractThe β subunit of human Ca2+/calmodulin-dependent protein kinase II (β CaMKII) was identified by searching through an expressed sequence tag database and rapid amplification of cDNA 5′-ends and was assigned to chromosome 7. Reverse transcription-polymerase chain reaction and sequencing analysis identified at least five alternative splicing variants of β CaMKII (β, β6, βe, β′e, and β7) in brain and two of them (β6 and β7) were first detected in any species. When expressed in HEK 293 cells, the Ca2+/calmodulin-dependent kinase activity of β7, the shortest variant, was much lower than that of either β (the longest one) or βe (the medium one), suggesting possible regulation of β CaMKII activity by alternative splicing

Clinical analysis of neovascular glaucoma after vitrectomy in patients with diabetic retinopathy

AIM: To explore the factors and analyze the occurrence of neovascular glaucoma(NVG)after vitrectomy for proliferative diabetic retinopathy(PDR). METHODS:Retrospective analysis on the clinical materials from January 2013 to December 2017, including 233 PDR patients(237 eyes)treated with vitrectomy in which 20 patients(20 eyes)occurred NVG after vitrectomy surgeries. The factors might lead to NVG were investigated. RESULTS: Totally 20 patients(20 eyes)developed NVG, in which there were 12 eyes combined with cataract surgeries, of the 12 eyes, 6 eyes had posterior lens capsule deficiency; 14 eyes complicated with hypertensions; 16 eyes complicated with hyperlipoidemia; 6 eyes had reoperations of vitrectomy surgeries; 2 eyes were silicone oil-filled eyes or silicone oil-removed eyes. There were correlations between the combinations of the cataract surgeries, posterior lens capsule deficiency, and vitrectomy with the occurrences of NVG after PDR surgeries; vitreous silicone oil filling might inhibit the occurrence of NVG to a certain extent. There was no statistical significance in hypertensions, high plasma lipids and reoperations of vitrectomy surgery for occurrence of NVG after PDR surgery. CONCLUSION: There is positive significance in correctly grasping the timing of lens surgery, and properly selecting the vitreous cavity filler

Effects of controlled atmosphere storage with nitrogen on yellowing and quality of high quality rice

High-quality rice with moisture content of 13.5%was tested at 35 ℃,40 ℃and 45 ℃storage with nitrogen,compared with the control group without nitrogen.The degree of yellowing of high-quality rice and the quality change during the process of yellowing were researched by detecting yellowness index,fatty acid value,brown rice rate and head rice yield.The results showed that high temperature can cause yellowing of high quality rice.The higher the temperature,the higher the yellowness index,the deeper the yellowing degree.After storage at 35 ℃,40 ℃and 45 ℃for 45 days,yellowness index with nitrogen was 1.2,2.2 and 3.0 lower than that without nitrogen,which indicated that nitrogen gas regulation can effectively reduce the yellowness index.In the process of high-quality rice yellowing,the temperature was proportional to the fatty acid value,but inversely proportional to brown rice rate and head rice yield.After 45 days of storage,the difference of quality of the rice between nitrogen group and control group was most obviously,in which the fatty acid value of the nitrogen group was 7 mgKOH/100g lower than that of the control group,the brown rice rate was 0.20% higher and the head rice yield was 16.05% higher,which indicated that nitrogen gas can inhibit fatty acidsformation in high-quality rice,and decrease ofthe head rice yield,while have a little affection on brown rice rate.Therefore,in thepractical storage of high-quality rice,high temperature should be avoided as much as possible.If it is unavoidable,storage with nitrogen can be selected to postpone the yellowing and quality deterioration of high-quality rice

Tripartite quantum entanglement with squeezed optomechanics

The ability to engineer entangled states that involve macroscopic objects is of particular importance for a wide variety of quantum-enabled technologies, ranging from quantum information processing to quantum sensing. Here we propose how to achieve coherent manipulation and enhancement of quantum entanglement in a hybrid optomechanical system, which consists of a Fabry-P\'{e}rot cavity with two movable mirrors, an optical parametric amplifier (OPA), and an injected squeezed vacuum reservoir. We show that the advantages of this system are twofold: (i) one can effectively regulate the light-mirror interactions by introducing a squeezed intracavity mode via the OPA; (ii) when properly matching the squeezing parameters between the squeezed cavity mode and the injected squeezed vacuum reservoir, the optical input noises can be suppressed completely. These peculiar features of this system allow us to generate and manipulate quantum entanglement in a coherent and controllable way. More importantly, we also find that such controllable entanglement, under some specific squeezing parameters, can be considerably enhanced in comparison with those of the conventional optomechanical system. Our work, providing a promising method to regulate and tailor the light-mirror interaction, are poised to serve as a useful tool for engineering various quantum effects which are based on cavity optomechanics.Comment: 11 pages, 3 figure

Comparison of out-of-plane short axis with in-plane long axis for ultrasound-guided radial arterial cannulation: A systematic review with trial sequential analysis of randomised controlled trials

BackgroundIt is controversial whether the short-axis out-of-plane or long-axis in-plane approach is a better needling technique for ultrasound-guidance radial artery cannulation. We aimed to compare the efficacy and safety of the two approaches for ultrasound-guided radial artery cannulation.MethodsA systematic search of Medline, Embase, the Cochrane Library, and Web of Science for relevant articles published until 1 May 2021 was conducted. Randomised controlled trials comparing the long-axis in-plane with short-axis out-of-plane approaches were included. Review Manager software version 5.4, STATA version 14.2, and trial sequential analysis (TSA) version 0.9.5.10 Beta were used for statistical analysis. Risk of bias and methodological quality of all studies included in this review were assessed according to the Cochrane Collaboration tool for the risk of bias. Subgroup analyses and meta-regression were performed to explore sources of heterogeneity.ResultsThe rate of cannula insertion success on the first attempt was similar between the short-axis out-of-plane and long-axis in-plane approaches (RR = 1.03; 95% CI: 0.83 to 1.28; P = 0.79; I2 = 83.0%). No significant differences were observed in total time to successful cannulation between the two approaches (MD = –3.9; 95% CI:-18.30 to 10.49; P = 0.6; I2 = 97%). However, the required information size for the success rate of the first attempt and total time to successful cannulation was not reached.ConclusionIt remains inconclusive whether short-axis out-of-plane is a better choice for radial arterial cannulation than the long-axis in-plane approach. Inexperienced operators may need more attempts and longer ultrasound location time with the short-axis out-of-plane technique.Systematic review registration[https://www.crd.york.ac.uk/prospero/], identifier [CRD42021236098]

Research on the vibration characteristics of the commercial-vehicle cabin based on experimental design and genetic algorithm

Most of the published researches on the vibration isolation performance of the commercial vehicle focus on the suspension system and the power-train system, and few of them focus on the cabin. Moreover, the researches on the cabin mostly focus on how to reduce the interior noise, and the interior vibration characteristics have not received adequate attention. Therefore, this paper tries to research the vibration characteristics of the commercial-vehicle cabin in order to improve ride comfort. Firstly, the vibration characteristic of the cabin was tested on the actual road. Then, the rigid-flexible coupling dynamic model of the commercial vehicle was built, and the computational results were compared with the experimental results. It was found that the results were consistent with each other, which showed that the computational model was reliable. Finally, based on the verified computation model, some parameters that influenced the vibration characteristic of the cabin were explored. As a result, the influencing tendency of each parameter to the cabin vibration under each working condition was obtained, but influencing levels of each parameter cannot be analyzed quantitatively. In order to research the contribution degree of each parameter to the vibration in cabin, DOE (Design of Experiment) method was used for the analysis to provide references for the optimal matching of a cabin suspension system. Then, the vibration in the cabin was optimized based on genetic algorithm to obtain the optimal performance. This research can provide a reference for the other researches on the reduction vibration for the cabin

Study on the yellowing of high quality japonica rice before entering the warehouse after harvesting

Through the simulation of different climatic conditions (clear,cloudy day and rainy day) before the storage of high-quality japonica rice under laboratory conditions,the yellowing of freshly harvested high-quality japonica rice cultivar Nanjing 9108 under different climates was studied,and the yellowing was observed.The quality changes in the process were measured.The results showed that yellowing did not occur in 35 days at 25 ℃and 30 ℃under sunny conditions,and in the process of yellowing,the yellow-coloured index from 28.02 to 31.02,the fatty acid value was less than 25 mg KOH/100g,the husked rice yield was more than 81%,and head rice yield was less than 55%.Under rainy conditions,80%RH,25 ℃and 30 ℃,there was no yellowing in 35 days.the heat reached 40 ℃,yellowing occured in 20 to 25 days.the heat reached 50 ℃,yellowing occurred in 15 days.90%RH,25 ℃and 30 ℃,yellowing occurred in 35 days.the heat reached 40 ℃,yellowing occurred in 15 to 20 days,the heat reached 40 ℃,yellowing occurred in 15 days； During the yellowing process,the yellow-coloured index from 28.03 to 32.69,the change of fatty acid value was obvious and,followed by the husked rice yield,the first grade of Japonica rice was reduced to the second grade,and the head rice yield was lower than 50%,which was less than the requirement of the third grade of high quality Japonica rice

苏州大学中、外医学生对控烟KAP的比较研究

Objective: Based on the survey of the knowledge-attitude-belief and practice to tobacco control between Chinese and foreign medical students in Soochow University, the authors intended to explore the current situation, differences and influencing factors of Chinese and foreign medical students, in order to provide a reference for tobacco control policies and measures set by the Medical College of Soochow University. Methods: By stratified sampling, we selected 200 Chinese medical students and 200 foreign medical students of Medical College in different grades and different majors, and adopted anonymous questionnaires to investigate. Results: The smoking rates of Chinese and foreign male medical students were 7.4%, 15.5% respectively , while female medical students' smoking rates were 0.0, 8.1% respectively. Foreign medical students’ daily smoking was mostly 4~10, which was significantly greater than the Chinese medical students （P<0.05）; besides, the identification degree to the responsibilities and duties for smoking control of the professional medical staff were both higher. The influence of the different smoking rates between Chinese and foreign medical students was mainly ethnic background, and the influences of the different smoking behavior mainly depended on their knowledge and attitude towards tobacco control. Conclusion: There is a need for schools to adopt health education measure to promote the school tobacco control based on the differences of the knowledge-attitude-belief and practice to tobacco control between Chinese and Foreign Medical students. Help medical students to translate knowledge of tobacco control they have already mastered into belief, and further affect their behavior.目的  通过对苏州大学中、外医学生关于控烟知信行的调查，探讨中、外医学生控烟知信行的现状、差异及影响因素，为苏州大学医学部制定控烟政策和措施提供参考依据。方法  采用分层随机抽样的方法抽取医学部不同年级不同专业中国与外国医学生各200人，采用自填式匿名问卷进行调查。结果  中、外男性医学生现在吸烟率分别为7.4%、15.5%，女性医学生吸烟率分别为0.0%、8.1%。外国医学生吸烟者的日均吸烟量多为4~10支，显著大于中国医学生的2~3支（P<0.05）；中、外医学生对职业医务人员控烟责任与义务的认同度都比较高。影响中、外医学生吸烟率的主要因素是民族背景，影响吸烟行为的主要因素是对控烟知识的了解以及态度。结论  学校应针对中、外医学生的知、信、行差异综合采取健康教育策略，促进校园控烟工作。帮助医学生将所掌握的控烟知识内化为信念，进而改变不良行为