Difference between Mitochondrial RNase P and Nuclear RNase P [Letters to the Editor]

[Discussion of: Puranam, R. S., and G. Attardi. 2001. The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the nuclear RNase P. Mol. Cell. Biol. 21:548-561.

Antimicrobial activity and Escherichia coli biofilm destruction potency of Siddha formulation Sagadevinei

536-540The primary objective is to evaluate the microbial efficacy and biofilm destruction potency of Siddha polyherbal formulation “Sagadevinei’’ and secondary objective is to correlate the ingredients with Siddha principles such as Suvai (Taste) and Panchabootha thathuvam (five elements theory). The In vitro microbial activity of Sagadevinei (SDN) has been carried out through Agar well diffusion method and evaluated the Escherichia coli biofilm destruction potency, by growing of Escherichia coli to form biofilm in coverslip. The coverslip insisted with biofilm were stained by Acridine Orange (AO Staining), and the potency has observed under fluorescent microscope. The result shows significant microbial activity in five common microorganisms and the drug revealed marked potency against E. coli strain. Further bio-film destruction potency of the test drug was also carried out and discussed. There is a huge need for a drug from herbal origin to tackle the situation of a post antibiotic era. In day to day clinical practice, almost 80% of cases are from Escherichia coli, Gram-negative bacteria alone. To overcome the antibiotic resistance, reinfection, and reversion, the novel herbal replacements are quite effective in nature, easily affordable and safe

Antimicrobial activity and Escherichia coli biofilm destruction potency of Siddha formulation Sagadevinei

The primary objective is to evaluate the microbial efficacy and biofilm destruction potency of Siddha polyherbal formulation “Sagadevinei’’ and secondary objective is to correlate the ingredients with Siddha principles such as Suvai (Taste) and Panchabootha thathuvam (five elements theory). The In vitro microbial activity of Sagadevinei (SDN) has been carried out through Agar well diffusion method and evaluated the Escherichia coli biofilm destruction potency, by growing of Escherichia coli to form biofilm in coverslip. The coverslip insisted with biofilm were stained by Acridine Orange (AO Staining), and the potency has observed under fluorescent microscope. The result shows significant microbial activity in five common microorganisms and the drug revealed marked potency against E. coli strain. Further bio-film destruction potency of the test drug was also carried out and discussed. There is a huge need for a drug from herbal origin to tackle the situation of a post antibiotic era. In day to day clinical practice, almost 80% of cases are from Escherichia coli, Gram-negative bacteria alone. To overcome the antibiotic resistance, reinfection, and reversion, the novel herbal replacements are quite effective in nature, easily affordable and safe

Crop Insurance and Disaster Payments.

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Import of ADP/ATP carrier into mitochondria

We have identified the yeast homologue of Neurospora crassa MOM72, the mitochondrial import receptor for the ADP/ATP carrier (AAC), by functional studies and by cDNA sequencing. Mitochondria of a yeast mutant in which the gene for MOM72 was disrupted were impaired in specific binding and import of AAC. Unexpectedly, we found a residual, yet significant import of AAC into mitochondria lacking MOM72 that occurred via the receptor MOM19. We conclude that both MOM72 and MOM19 can direct AAC into mitochondria, albeit with different efficiency. Moreover, the precursor of MOM72 apparently does not require a positively charged sequence at the extreme amino terminus for targeting to mitochondria

Whole-cell detection of live lactobacillus acidophilus on aptamer-decorated porous silicon biosensors

This work describes the design of optical aptamer-based porous silicon (PSi) biosensors for the direct capture of Lactobacillus acidophilus. Aptamers are oligonucleotides (single-stranded DNA or RNA) that can bind their targets with high affinity and specificity, making them excellent recognition elements for biosensing applications. Herein, aptamer Hemag1P, which specifically targets the important probiotic L. acidophilus, was utilized for direct bacteria capture onto oxidized PSi Fabry-Perot thin films. Monitoring changes in the reflectivity spectrum (using reflective interferometric Fourier transform spectroscopy) allows for bacteria detection in a label-free, simple and rapid manner. The performance of the biosensor was optimized by tuning the PSi nanostructure, its optical properties, as well as the immobilization density of the aptamer. We demonstrate the high selectivity and specificity of this simple "direct-capture" biosensing scheme and show its ability to distinguish between live and dead bacteria. The resulting biosensor presents a robust and rapid method for the specific detection of live L. acidophilus at concentrations relevant for probiotic products and as low as 10(6) cells per mL. Rapid monitoring of probiotic bacteria is crucial for quality, purity and safety control as the use of probiotics in functional foods and pharmaceuticals is becoming increasingly popular.DFG/CHE 279/32-