Comparison of spatial rainfall data calculated with a meteorological model and from interpolation of measurements - implications for FRAME modelled wet deposition

This work quantifies the differences between the two sources of spatial information of precipitation on FRAME modelled wet deposition of oxidised sulphur (SOx), nitrogen (NOy) and reduced nitrogen (NHx) for two years in an area of Poland. The model was run twice, first with precipitation dataset calculated with the WRF model (pWRF). The second run was based on interpolation of measured precipitation with kriging (pOK). SOx, NOy and NHx wet deposition calculated with pWRF precipitation gives lower country mean values if compared with pOK. The maximum values are higher for pWRF precipitation. Grid to grid correlation between pOK and pWRF modelled wet deposition is similar for both years, with the lowest values for NHx and the highest for SOx. The model-measurement agreement is better for the pOK FRAME run. The results show large uncertainties related with wet deposition modelling due to uncertainties in rainfall data

Application of WRF-Chem to forecasting PM10 concentration over Poland

The meteorological and chemical transport model WRF-Chem was implemented to forecast PM10 concentrations over Poland. WRF-Chem version 3.5 was configured with three one-way nested domains using the GFS meteorological data and the TNO MACC II emissions. The 48 hour forecasts were run for each day of the winter and summer period of 2014 and there is only a small decrease in model performance for winter with respect to forecast lead time. The model in general captures the variability in observed PM10 concentrations for most of the stations. However, for some locations and specific episodes, the model performance is poor and the results cannot yet be used by official authorities. We argue that a higher resolution sector-based emission data will be helpful for this analysis in connection with a focus on planetary boundary layer processes in WRF-Chem and their impact on the initial distribution of emissions on both time and space

Profitability of coal mining in Poland and its socio- economic consequences

Węgiel kamienny stanowi gwarant bezpieczeństwa energetycznego Polski i wydaje się być nieodzownym elementem naszej gospodarki. W ciągu ostatnich lat, na sku-tek polityki krajowej oraz polityki Unii Europejskiej, zapotrzebowanie na ten suro-wiec znacznie spada, a samo wydobycie jest coraz mniej rentowne. W niniejszej pracy dokonano charakterystyki węgla kamiennego oraz sektora wydobycia w Pol-sce, przedstawiono negatywne efekty zewnętrzne dla społeczeństwa i środowiska naturalnego, a także zaprezentowano alternatywne źródła pozyskiwania energii elektrycznej.Hard coal is the crucial resource for Polish energy security and it seems to be an in-evitable part of our economy. During the last few years, due to both national and European legislations, demand for the coal has decreased. Moreover, coal mining is less rentable than it was in the past. In this Master Thesis the detailed characteris-tics of the fuel has been made and negative side effects for the environment and the society have been presented. Additionally, some alternative sources of energy have been discussed

The impact of precipitation on wet deposition of sulphur and nitrogen compounds

Atmospheric transport model FRAME has been used in this study to estimate the influence of precipitation on the patterns of wet deposition of oxidised sulphur, oxidised nitrogen and reduced nitrogen in Poland during the years 1981-2005. A constant wind and emission data and year-specific spatially interpolated precipitation data was used in the model. The results show that the correlation coefficient between mean annual precipitation totals and mean wet deposition is above 0.9 for all examined compounds. The spatial patterns of pollutant deposition are similar for all years, with the north-western part of Poland receiving the lowest and the southern, mountainous part, the highest pollutant load. The largest precipitation-induced changes in wet deposition budgets are observed for oxidised sulphur (53% of the average amount between wet and dry year), and smaller for oxidised and reduced nitrogen (30%). Inter-annual precipitation changes cause large variations in the amount of wet deposition of pollutants. This means that the emission abatements may not cause immediate environmental effects, eg reductions in deposition of pollutants and, further ecosystems areas of exceeded critical loads

Physiological Hypoxia (Physioxia) Impairs the Early Adhesion of Single Lymphoma Cell to Marrow Stromal Cell and Extracellular Matrix. Optical Tweezers Study

Adhesion is critical for the maintenance of cellular structures as well as intercellular communication, and its dysfunction occurs prevalently during cancer progression. Recently, a growing number of studies indicated the ability of oxygen to regulate adhesion molecules expression, however, the influence of physiological hypoxia (physioxia) on cell adhesion remains elusive. Thus, here we aimed: (i) to develop an optical tweezers based assay to precisely evaluate single diffuse large B-cell lymphoma (DLBCL) cell adhesion to neighbor cells (mesenchymal stromal cells) and extracellular matrix (Matrigel) under normoxia and physioxia; and, (ii) to explore the role of integrins in adhesion of single lymphoma cell. We identified the pronouncedly reduced adhesive properties of lymphoma cell lines and primary lymphocytes B under physioxia to both stromal cells and Matrigel. Corresponding effects were shown in bulk adhesion assays. Then we emphasized that impaired β1, β2 integrins, and cadherin-2 expression, studied by confocal microscopy, account for reduction in lymphocyte adhesion in physioxia. Additionally, the blockade studies conducted with anti-integrin antibodies have revealed the critical role of integrins in lymphoma adhesion. To summarize, the presented approach allows for precise confirmation of the changes in single cell adhesion properties provoked by physiological hypoxia. Thus, our findings reveal an unprecedented role of using physiologically relevant oxygen conditioning and single cell adhesion approaches when investigating tumor adhesion in vitro