Architectuur standaard raamwerk water

Dit rapport beschrijft de architectuur zoals die ontwikkeld is voor een standaardraamwerk water. Dit raamwerk beoogt een generieke omgeving voor simulatiemodellen in het integraal waterbeheer te zijn. Dit rapport bevat een analyse van het domein simulatiemodellen in het integraal waterbeheer, een ontwerp van de informatiearchitectuur en aanbevelingen om tot een dergelijk generiek raamwerk te komen

Creating space for biodiversity by planning swath patterns and field marging using accurate geometry

Potential benefits of field margins or boundary strips include promotion of biodiversity and farm wildlife, maintaining landscape diversity, exploiting pest predators and parasites and enhancing crop pollinator populations. In this paper we propose and demonstrate a method to relocate areas of sub-efficient machine manoeuvring to boundary strips so as to optimise the use of available space. Accordingly, the boundary strips will have variable rather than fixed widths. The method is being tested in co-operation with seven farmers in the Hoeksche Waard within the province of Zuid Holland, The Netherlands. In a preliminary stage of the project, tests were performed to determine the required accuracy of field geometry. The results confirmed that additional data acquisition using accurate measuring devices is required. In response, a local contracting firm equipped a small all-terrain vehicle (quad) with an RTK-GPS receiver and set up a service for field measurement. Protocols were developed for requesting a field measurement and for the measurement procedure itself. Co-ordinate transformation to a metric system and brute force optimization of swath patterns are achieved using an open source geospatial library (osgeo.ogr) and Python scripting. The optimizer basically tests all orientations and relevant intermediate angles of input field boundaries and tries incremental positional shifts until the most efficient swath pattern is found. Inefficient swaths intersecting boundary areas are deleted to create space for field margins. The optimised pattern can be forwarded to an agricultural navigation system. At the time of the conference, the approach will have been tested on several farm fields

Quantitative live-cell imaging and computational modelling shed new light on endogenous WNT/CTNNB1 signaling dynamics

WNT/CTNNB1 signaling regulates tissue development and homeostasis in all multicellular animals, but the underlying molecular mechanism remains incompletely understood. Specifically, quantitative insight into endogenous protein behavior is missing. Here, we combine CRISPR/Cas9-mediated genome editing and quantitative live-cell microscopy to measure the dynamics, diffusion characteristics and absolute concentrations of fluorescently tagged, endogenous CTNNB1 in human cells under both physiological and oncogenic conditions. State-of-the-art imaging reveals that a substantial fraction of CTNNB1 resides in slow-diffusing cytoplasmic complexes, irrespective of the activation status of the pathway. This cytoplasmic CTNNB1 complex undergoes a major reduction in size when WNT/CTNNB1 is (hyper)activated. Based on our biophysical measurements, we build a computational model of WNT/CTNNB1 signaling. Our integrated experimental and computational approach reveals that WNT pathway activation regulates the dynamic distribution of free and complexed CTNNB1 across different subcellular compartments through three regulatory nodes: the destruction complex, nucleocytoplasmic shuttling, and nuclear retention