Ultrastructure of Escherichia coli cells under the action of a novel derivative of aryl aliphatic aminoalcohols.

Background. Novel derivatives of aryl aliphatic aminoalcohols were examined for antimicrobial activity. Compound KVM-114 (4-(1,1,3,3-tetrabutyl) phenoxy-3-dimethylamino-2-propanol hydrochloride) was found as a selective against gram-negative bacteria. Objective. Investigation of compound KVM-114 influence on E. coli ultrastructure. Methods. Minimum inhibitory concentrations were determined by serial dilution method in Muller-Hinton broth. Bacteria (Escherichia coli) for transmission electron microscopy samples preparation was grown to exponential phase and then was exposed to the subinhibitory concentration of KVM-114 for 1 h and 24 h. Results. Intact E. coli cells were rod-shaped with rounded ends. A light layer, allowing clear visualization of the cell wall, was observed between the сytoplasm and cytoplasmic membrane. Lipopolysaccharide layer was well distinguished as well. Cytoplasm was filled with ribosomes and polyribosomes. 1 hour exposition to KVM-114 at a subinhibitory concentration resulted in the absence of polyribosomes in the cytoplasm. Increase of electron density of lipopolysaccharide layer and cell wall indicate alteration of cell envelope. Prolongation of the incubation period to 24 hours led to cell recovery: no changes were observed, compared to control cells. The data obtained suggest compound’s ability to alter cell envelope and metabolic activity, however, subinhibitory concentration is apparently not sufficient for total inhibition of E. coli growth. Conclusion. The derivative of aryl aliphatic aminoalcohols, compound KVM-114, possesses inhibitory activity against gram-negative bacteria. E. coli treatment with this compound resulted in structural changes of the cell wall and alteration of intracellular processes

Ультраструктура Escherichia coli при дії нового похідного арилаліфатичних аміноспиртів

Background. Novel derivatives of aryl aliphatic aminoalcohols were examined for antimicrobial activity. Compound KVM-114 (4-(1,1,3,3-tetrabutyl) phenoxy-3-dimethylamino-2-propanol hydrochloride) was found as a selective against gram-negative bacteria. Objective. Investigation of compound KVM-114 influence on E. coli ultrastructure. Methods. Minimum inhibitory concentrations were determined by serial dilution method in Muller-Hinton broth. Bacteria (Escherichia coli) for transmission electron microscopy samples preparation was grown to exponential phase and then was exposed to the subinhibitory concentration of KVM-114 for 1 h and 24 h. Results. Intact E. coli cells were rod-shaped with rounded ends. A light layer, allowing clear visualization of the cell wall, was observed between the сytoplasm and cytoplasmic membrane. Lipopolysaccharide layer was well distinguished as well. Cytoplasm was filled with ribosomes and polyribosomes. 1 hour exposition to KVM-114 at a subinhibitory concentration resulted in the absence of polyribosomes in the cytoplasm. Increase of electron density of lipopolysaccharide layer and cell wall indicate alteration of cell envelope. Prolongation of the incubation period to 24 hours led to cell recovery: no changes were observed, compared to control cells. The data obtained suggest compound’s ability to alter cell envelope and metabolic activity, however, subinhibitory concentration is apparently not sufficient for total inhibition of E. coli growth. Conclusion. The derivative of aryl aliphatic aminoalcohols, compound KVM-114, possesses inhibitory activity against gram-negative bacteria. E. coli treatment with this compound resulted in structural changes of the cell wall and alteration of intracellular processes.Целью работы было установить изменения ультраструктуры кишечной палочки при действии нового производного арилалифатических аминоспиртов соединения КВМ-114. Исследования проведены с использованием метода трансмиссионной электронной микроскопии. Установлено, что при инкубации клеток с соединением в субингибирующей концентрации уже через 1 ч наблюдаются нарушения структуры клеточной оболочки и изменения, свидетельствующие об угнетении внутриклеточных процессов.Метою роботи було встановити зміни ультраструктури кишкової палички при дії нового похідного арилаліфатичних аміноспиртів, сполуки КВМ-114. Дослідження проведені з використанням методу трансмісійної електронної мікроскопії. Встановлено, що при інкубації клітин зі сполукою у субінгібуючій концентрації вже через 1 рік спостерігаються порушення структури клітинної оболонки та зміни, що свідчать про пригнічення внутрішньоклітинних процесів

THE REACTION OF PHOSPHORUS-CONTAINING INTRACELLULAR INCLUSIONS TO SPACE WEATHER CHANGES

It is known that the bio-astronomical effect of Chizhevsky-Velhover is based on the cytochemical metachromatic reaction of polyphosphatecontaining volutin granules of microorganisms. As a result of long-term monitoring (2001-2015 years) of this of cytochemical index in yeasts Saccharomyces cerevisiae, it was shown that metachromatic staining of the granules possessed rhythmicity and connection with space weather. Under laboratory conditions the change of metachromasy was achieved by applying different stress factors: anaerobiosis, low temperature, acidity. Simulation of metachromatic staining in vitro by using solutions of inorganic polyphosphate with other compounds showed that this reaction was possible for the low concentrations of these polymers. The effect of the low concentrations was more expressed with increasing chain length of polyphosphate. The inhibition of the metachromasy in solution with added protein and calcium chloride indicated that this of reaction involved the presence of the protonated sites in the polymers. It is assumed that metachromatic staining of volutin granules in vivo may depend on other conditions including conformational restructuring of a polymer or sol-gel phase transitions. Thus, the question whether the metachromatic reaction of intracellular polyphosphate granules to the space weather changes is driven by a response to associated stress or despite the apparent similarity of the effects they are of a different nature remains unclear