ZeroWasteWater: short-cycling of wastewater resources for sustainable cities of the future

Sewage treatment relies mainly on conventional activated sludge (CAS) systems, reaching sufficiently low pollutant effluent levels. Yet, CAS has a low cost-effectiveness and recovery potential and a high electricity demand and environmental footprint. By 2050, globally we have to solve severe water and phosphorus shortages while significantly decreasing greenhouse gas emissions. In this review and opinion paper, the ZeroWasteWater concept is proposed as a sustainable centralised technology train to short-cycle water, energy and valuable materials from sewage, while adequately abating pathogens, heavy metals and trace organics. Electrical energy recovery from anaerobic digestion of the organics present in sewage and kitchen waste (KW) has a value of 4.0 per inhabitant equivalent (IE) per year. In addition to sewerage improvements and water conservation, prerequisites include an advanced physico-chemical and/or biological concentration step at the entry of the sewage treatment plant. In the side stream, the recovery of phosphorus and carbon-sequestrating biochar from the digested sludge and of nitrogen from the digestate has a value of 6.3IE-1 year-1. Alternatively, recovery of biogas and materials can occur directly on source-separated black water. In the main stream, partial nitritation and anammox oxidise residual nitrogen. Moreover, two serial heat pumps recover thermal energy, valued at 6.9IE-1 year-1, cooling the water by 5 degrees C, and membrane technologies recover potable water at 65IE-1 year-1. Interestingly, ZeroWasteWater is expected to be economically viable. Key steps are to incorporate water chain management into holistic urban planning and thus produce a cradle-to-cradle approach that society will find acceptable

Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture

Milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 C-18:1 and trans-10, cis-12 conjugated linoleic acid (CLA) in milk. These fatty acids (FA) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated FA. Because a reduction in ruminal pH is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. The effect of pH (6.4 vs. 5.6) and dietary forage to concentrate ratios (F:C; 70:30 F:C vs. 30:70 F:C) on rumen microbial fermentation, effluent FA profile, and DNA concentration of bacteria involved in lipolysis and biohydrogenation processes were investigated in a continuous culture trial. The dual-flow continuous culture consisted of 2 periods of 8 d (5 d for adaptation and 3 d for sampling), with a 2 x 2 factorial arrangement of treatments. Samples from solid and liquid mixed effluents were taken for determination of total N, ammonia-N, and volatile fatty acid concentrations, and the remainder of the sample was lyophilized. Dry samples were analyzed for dry matter, ash, neutral and acid detergent fiber, FA, and purine contents. The pH 5.6 reduced organic matter and fiber digestibility, ammonia-N concentration and flow, and crude protein degradation, and increased nonammonia and dietary N flows. The pH 5.6 decreased the flow of C-18:0, trans-11 C-18:1 and cis-9, trans-11 CLA, and increased the flow of trans-10 C-18:1, C18:2n-6, C18:3n-3, trans-11, cis-15 C-18:2 and trans-10, cis-12 CLA in the 1 h after feeding effluent. The pH 5.6 reduced Anaerovibrio lipolytica (32.7 vs. 72.1 pg/10 ng of total DNA) and Butyrivibrio fibrisolvens vaccenic acid subgroup (588 vs. 1,394 pg/10 ng of total DNA) DNA concentrations. The high-concentrate diet increased organic matter and fiber digestibility, nonammonia and bacterial N flows, and reduced ammonia-N concentration and flow. The high-concentrate diet reduced trans-11 C-18:1 and trans-10 C-18:1, and increased C18:2n-6, C18:3n-3 and trans-10, cis-12 CLA proportions in the 1 h after feeding effluent. The increase observed in trans-10, cis-12 CLA proportion in the 1 h after feeding effluent due to the high-concentrate diet was smaller that that observed at pH 5.6. Results indicate that the pH is the main cause of the accumulation of trans-10 C-18:1 and trans-10, cis-12 CLA in the effluent, but the trans-10, cis-12 CLA proportion can be also affected by high levels of concentrate in the diet

Increased salinity improves the thermotolerance of mesophilic nitrification

Nitrification is a well-studied and established process to treat ammonia in wastewater. Although thermophilic nitrification could avoid cooling costs for the treatment of warm wastewaters, applications above 40 A degrees C remain a significant challenge. This study tested the effect of salinity on the thermotolerance of mesophilic nitrifying sludge (34 A degrees C). In batch tests, 5 g NaCl L-1 increased the activity of aerobic ammonia-oxidizing bacteria (AerAOB) by 20-21 % at 40 and 45 A degrees C. For nitrite-oxidizing bacteria (NOB), the activity remained unaltered at 40 A degrees C, yet decreased by 83 % at 45 A degrees C. In a subsequent long-term continuous reactor test, temperature was increased from 34 to 40, 42.5, 45, 47.5 and 50 A degrees C. The AerAOB activity showed 65 and 37 % higher immediate resilience in the salt reactor (7.5 g NaCl L-1) for the first two temperature transitions and lost activity from 45 A degrees C onwards. NOB activity, in contrast to the batch tests, was 37 and 21 % more resilient in the salt reactor for the first two transitions, while no difference was observed for the third temperature transition. The control reactor lost NOB activity at 47.5 A degrees C, while the salt reactor only lost activity at 50 A degrees C. Overall, this study demonstrates salt amendment as a tool for a more efficient temperature transition for mesophilic sludge (34 A degrees C) and eventually higher nitrification temperatures

The nitrogen and phosphorus budget of Flanders: a tool for efficient waste management and nutrient recovery

The region of Flanders in Belgium is, due to its high population density, intensive industry and livestock production, a nutrient-rich region. This results in important anthropogenic emissions to the environment, but also a large potential for the recovery and reuse of nitrogen (N ) and phosphorus (P) from waste streams. In this study, a substance flow analysis study for N and P is presented, in which the anthropogenic fluxes, stocks and hot spots of these two nutrients are quantified throughout the Flemish economy and environment. The environmental impact of the different economic sectors is addressed through the determination of the N and P footprint. The importance of food production in the nutrient cycle is thereby demonstrated through the large contribution of agriculture to the nutrient footprint (49% of N and 36% of P). Further focus is placed on the nutrient use efficiencies across the different sectors of the food supply nexus to target key nutrient losses and inefficiencies. This leads to an overall fertilizer-to-consumer efficiency of 14% for N and P, with the main nutrient losses originating from livestock production and food processing. At the end of the production and consumption chain, important nutrient quantities are embedded in concentrated waste streams such as excess manure, food processing waste streams and activated sludge. This demonstrates the large potential for nutrient recovery as a tool to improve nutrient use efficiencies and reduce the dependency of inorganic fertilizers. Several nutrient recovery strategies, both physicochemical and microbial, were evaluated for their economic feasibility and their impact on the primary energy demand of the total food supply chain

Media Optimization, Strain Compatibility, and Low-Shear Modeled Microgravity Exposure of Synthetic Microbial Communities for Urine Nitrification in Regenerative Life-Support Systems

Urine is a major waste product of human metabolism and contains essential macro- and micronutrients to produce edible microorganisms and crops. Its biological conversion into a stable form can be obtained through urea hydrolysis, subsequent nitrification, and organics removal, to recover a nitrate-enriched stream, free of oxygen demand. In this study, the utilization of a microbial community for urine nitrification was optimized with the focus for space application. To assess the role of selected parameters that can impact ureolysis in urine, the activity of six ureolytic heterotrophs (Acidovorax delafieldii, Comamonas testosteroni, Cupriavidus necator, Delftia acidovorans, Pseudomonas fluorescens, and Vibrio campbellii) was tested at different salinities, urea, and amino acid concentrations. The interaction of the ureolytic heterotrophs with a nitrifying consortium (Nitrosomonas europaea ATCC 19718 and Nitrobacter winogradskyi ATCC 25931) was also tested. Lastly, microgravity was simulated in a clinostat utilizing hardware for in-flight experiments with active microbial cultures. The results indicate salt inhibition of the ureolysis at 30 mS cm(-1), while amino acid nitrogen inhibits ureolysis in a strain-dependent manner. The combination of the nitrifiers with C. necator and V. campbellii resulted in a complete halt of the urea hydrolysis process, while in the case of A. delafieldii incomplete nitrification was observed, and nitrite was not oxidized further to nitrate. Nitrate production was confirmed in all the other communities; however, the other heterotrophic strains most likely induced oxygen competition in the test setup, and nitrite accumulation was observed. Samples exposed to low-shear modeled microgravity through clinorotation behaved similarly to the static controls. Overall, nitrate production from urea was successfully demonstrated with synthetic microbial communities under terrestrial and simulated space gravity conditions, corroborating the application of this process in space

The manufacturing microbe

In all industrialized countries, the manufacturing industry appears to be in difficulty: the costs of maintaining skilled workers to do complicated technical tasks are becoming a major hurdle. The fact that a majority of the young pro-fessionals will spend their whole lives just typing in front of a screen is worrying plenty of sociologists, particularly because of the simple fact that our brains apparently are sparked to creativity by being involved in complex han-dling – read manufacturing – exercises (Sennett, 2013). At this moment, the overall environmental biotech is undergoing a major shift, driven by the need to help to abate climate changes. Until now, it has been dealing with tasks of making unwanted compounds disappear: to degrade contaminants, to remove waste and to eliminate unwanted microbial propagules. In industrialized coun

Milk fatty acid composition and associated rumen lipolysis and fatty acid hydrogenation when feeding forages from intensively managed or semi-natural grasslands

In order to evaluate the effect of replacing intensive forage by semi-natural grassland products on rumen lipid metabolism and milk fatty acid composition, four lactating and rumen canulated Holstein cows were used in a 4×4 Latin square design. Four different diets were fed: diet 100 IM - 100% intensively managed silage (IM), diet 20 SPP - 80% IM plus 20% semi-natural but species poor silage (SPP), diet 60 SPP - 40% IM plus 60% SPP and diet 60 SPR - 40% IM plus 60% semi-natural species rich silage (SPR). The silages showed significant differences in total fat content and in proportions of C18:2 n-6 and C18:3 n-3. Despite the reduced dietary supply of C18:3 n-3 with diets 60 SPP and 60 SPR, differences in milk C18:3 n-3 were small, suggesting higher recoveries of C18:3 n-3. Presumably, the latter are related to a higher transfer efficiency of C18:3 n-3 from the duodenum to the mammary gland, since rumen biohydrogenation, estimated from rumen pool size and first order rumen clearance kinetics, were similar among diets. CLA c9t11 in milk from cows fed diet 60 SPR were almost doubled compared to feeding one of the other diets. This has been related to the partial inhibition of rumen biohydrogenation of C18:3 n-3 and/or C18:2 n-6, as suggested by the increased proportions of hydrogenation isomers and reduced stearic acid proportions in rumen pool samples. In conclusion, the results suggest that the use of semi-natural grasslands in the diet of the animals reduce to some extent complete rumen biohydrogenation, which leads to an increase in milk CLA