Outcome of challenge with SP Pilus-1 overexpressing mutant in RrgB321 immunized animals.

<p>Density of colonization in middle ear fluid in RrgB321 immunized and control chinchillas challenged with SP Taiwan19F-14 pMU1328_<i>Pc-rlrA</i> (100% pilus +).</p

Pilus expression of SP Taiwan19F-14 wt in nasal and middle ear washes compared to inoculum.

<p>Flow cytometry analysis of Pilus-1 expression in nasal wash (NW) [A] and middle ear Fluid (MEF) [B] following challenge with SP Taiwan19F-14 wt.</p

Nasopharyngeal colonization and middle ear challenge with SP Taiwan19F-14 wt and PI-1/PI-2 mutant.

<p>Density of colonization in nasopharynx (A) and middle ear fluid (B) in chinchillas challenged with SP Taiwan19F-14 wt (pilus +) or with Taiwan19F-14 ΔPI-1/PI-2 mutant.</p

Pilus expression of SP Taiwan19F-14 wt in nasal and middle ear washes of RrgB321 immunized and control animals.

<p>Flow cytometry analysis of Pilus-1 expression in nasal wash (NW) [A] and middle ear Fluid (MEF) [B] in RrgB321 immunized and control animals following challenge with SP Taiwan19F-14 wt.</p

Outcome of challenge with SP Taiwan19F-14 wt in RrgB321 immunized and control animals.

<p>Density of colonization in nasopharynx (a) and middle ear fluid (b) in RrgB321 immunized and control chinchillas challenged with SP Taiwan19F-14 wt (pilus +).</p

Evaluation of pilus expression on surface of <i>Streptococcus pneumoniae</i>.

<p>Flow cytometry on SP Taiwan19F-14 wt and its derivatives (ΔPI-1/PI-2 mutant and pMU1328_<i>Pc-rlrA</i> + strain) performed with pAb against RrgB. The percentage of pilus positive and negative bacteria for each strain was reported.</p