73 research outputs found
Leverbot in kaart gebracht
Dairy cows grazing outside, or fed indoors with fresh grass, are increasingly infected by liver fluke, a parasitic flatworm. Losses resulting from liver fluke disease are generally underestimated, but include growth retardation, reduced resistance, lower milk production, and rejection of infected livers at the slaughterhouse
Onderzoek naar leverbot deel I: Instrument voor bedrijfsanalyse op risicofactoren - leverbotbestrijding, risico's & kansen
Liver fluke infections are increasingly a bottleneck in the animal health of grazing (or fresh grass fed) ruminants. Liverfluke leads to disease with economic consequences and for lactating animals no anthelmintics are freely available. This project has been working on the development of the liver fluke instrument with the aim of providing dairy farmers insight and action perspective regarding the liverfluke situation on their farm
Onderzoek naar leverbot deel I: Instrument voor bedrijfsanalyse op risicofactoren - leverbotbestrijding, risico's & kansen
Liver fluke infections are increasingly a bottleneck in the animal health of grazing (or fresh grass fed) ruminants. Liverfluke leads to disease with economic consequences and for lactating animals no anthelmintics are freely available. This project has been working on the development of the liver fluke instrument with the aim of providing dairy farmers insight and action perspective regarding the liverfluke situation on their farm
Integrale diergezondheid: beheersing van leverbot
Leverbotinfecties zijn in toenemende mate een knelpunt in de diergezondheid van melkvee en andere herkauwers. Door de complexe infectiecyclus, weinig inzetbare geneesmiddelen, geringe beschikbare diagnostiek en incomplete terugkoppeling van informatie (afkeur lever), kan leverbot de gezondheid negatief beïnvloeden waardoor ook vaak productieverliezen kunnen optreden. Voor vee dat melk produceert voor menselijke consumptie zijn nagenoeg geen middelen meer toegestaan om leverbot te bestrijden. Resistentie tegen de beschikbare middelen, indien diercategorieën wel behandeld mogen worden, neemt toe. Veehouders hebben behoefte aan kennis en mogelijkheden ter preventie van leverbotbesmettingen, en aan alternatieven voor een effectieve bestrijding van de leverbot en het voorkomen van resistentie tegen leverbotmiddelen. Vanuit deze problematiek gezien hebben veehouders behoefte aan een instrument waarmee ze de leverbotsituatie op hun bedrijf in kaart kunnen brengen en aan handelingsperspectief om de situatie te verbeteren. Dit project heeft een concept instrument ontwikkeld om de leverbotstatus op het bedrijf te beoordelen, de risicofactoren in kaart te brengen en mogelijke preventieve maatregelen aan te geven.
Dit onderzoek is uitgevoerd door Wageningen UR Livestock Research in samenwerking met het Louis Bolk Instituut en orgANIMprove, in opdracht van en gefinancierd door het Ministerie van Economische Zaken, in het kader van het Beleidsondersteunend onderzoek thema ‘Biologische landbou
Wat zijn de mogelijkheden om een leverbotinfectie van melkvee te voorkomen?
Infecties met leverbot zijn in toenemende mate een knelpunt in de diergezondheid van grazende (of vers gras gevoerde) herkauwers. Leverbotinfectie leidt tot ziekte met economische gevolgen en voor melkgevende dieren zijn geen anthelmintica vrij beschikbaar. In dit project is het leverbotinstrument ontwikkeld met als doel om veehouders inzicht en handelingsperspectief te geven ten aanzien van de leverbotsituatie op hun bedrijf. In dit rapport worden de resultaten weergegeven van het leverbotinstrument en een drietal preventieve maatregelen die zijn uitgeprobeerd
Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase
Transglutaminases (TGases) are calcium-dependent enzymes catalysing the
post-translational cross-linking of proteins. In the prostate at least two
TGases are present, the ubiquitously expressed tissue-type TGase (TGC),
and a prostate-restricted TGase (TGP). This paper deals with the molecular
cloning and characterization of the cDNA encoding the human prostate TGase
(hTGP). For this purpose we have screened a human prostate cDNA library
with a probe from the active-site region of TGC. The largest isolated cDNA
contained an open reading frame encoding a protein of 684 amino acids with
a predicted molecular mass of 77 kDa as confirmed by in vitro
transcription-translation and subsequent SDS/PAGE. The hTGP gene was
tissue-specifically expressed in the prostate, yielding an mRNA of approx.
3.5 kb. Furthermore, a 3-fold androgen-induced upregulation of hTGP mRNA
expression has been demonstrated in the recently developed human prostate
cancer cell line, PC346C. Other well established human prostate cancer
cell lines, LNCaP and PC-3, showed no detectable hTGP mRNA expression on a
Northern bolt. The gene coding for prostate TGase was assigned to
chromosome 3
Hypermethylation of CTDSPL2 prior to necrotizing enterocolitis onset
Background: Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in preterm infants. Epigenetic changes in DNA methylation may be present prior to NEC onset. Methods: 24 preterm infants with NEC and 45 matched controls were included. Human DNA was isolated from stool samples and methylation of CTDSPL2, HERC1, NXPE3 and PTGDR was measured using pyrosequencing. Results: CTDSPL2 displayed a higher DNA methylation of 51% compared with 17% in controls, prior to NEC onset (p = 0.047). Discussion: Noninvasive measurement of methylation in stool allows for comparison with healthy preterm controls. This potentially allows future biomarker or risk predictor use. The effect of CTDSPL2 hypermethylation on gene expression remains unclear. Plain language summary What is this article about? Necrotizing enterocolitis (NEC) is a common emergency condition affecting the gastrointestinal system of preterm infants. Epigenetic changes in DNA methylation may be present in infants before the onset of NEC. DNA methylation is a natural process that can help turn genes on or off, thereby affecting their function. This study focused on measuring the amount of DNA methylation in certain genes in preterm infants who developed NEC. What were the results? This study included 24 preterm infants with NEC and 45 matched healthy controls. The researchers isolated human DNA from stool samples, and the amount of DNA methylation of four specific genes was measured. They found that one of the genes, CTDSPL2, had significantly higher DNA methylation in infants who later developed NEC than in healthy infants. What do the results of the study mean? In this study, researchers found that CTDSPL2 showed a higher level of DNA methylation in stool samples of infants who later developed NEC. The effect of this change remains unclear, but may affect the way cells grow and respond to injury or infection, which could contribute to the development of NEC. Measuring DNA methylation in stool samples provides a noninvasive method for identifying DNA methylation changes in preterm infants. Comparing the amount of DNA methylation in healthy infants with that in preterm infants at risk of NEC may help predict the risk of developing NEC. </sec. Tweetable abstract DNA samples from infants show higher DNA methylation of the marker gene CTDSPL2 prior to necrotizing enterocolitis #NEC onset.</p
Reducing anthelmintic inputs in organic farming: Are small ruminant farmers integrating alternative strategies to control gastrointestinal nematodes?
Reducing anthelmintic inputs in organic farming: Are small ruminant farmers integrating alternative strategies to control gastrointestinal nematodes?
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