Estimativa de áreas de soja usando superfícies espectro-temporais derivadas de imagens MODIS em Mato Grosso, Brasil

The objective of this work was to evaluate the application of the spectral-temporal response surface (STRS) classification method on Moderate Resolution Imaging Spectroradiometer (MODIS, 250 m) sensor images in order to estimate soybean areas in Mato Grosso state, Brazil. The classification was carried out using the maximum likelihood algorithm (MLA) adapted to the STRS method. Thirty segments of 30x30 km were chosen along the main agricultural regions of Mato Grosso state, using data from the summer season of 2005/2006 (from October to March), and were mapped based on fieldwork data, TM/Landsat-5 and CCD/CBERS-2 images. Five thematic classes were considered: Soybean, Forest, Cerrado, Pasture and Bare Soil. The classification by the STRS method was done over an area intersected with a subset of 30x30-km segments. In regions with soybean predominance, STRS classification overestimated in 21.31% of the reference values. In regions where soybean fields were less prevalent, the classifier overestimated 132.37% in the acreage of the reference. The overall classification accuracy was 80%. MODIS sensor images and the STRS algorithm showed to be promising for the classification of soybean areas in regions with the predominance of large farms. However, the results for fragmented areas and smaller farms were less efficient, overestimating oybean areas.O objetivo deste trabalho foi avaliar a aplicação do método de classificação por superfícies de resposta espectro-temporal (STRS) em imagens do sensor Moderate Resolution Imaging Spectroradiometer (MODIS, 250 m) para estimar áreas de plantio de soja no Estado de Mato Grosso, Brasil. A classificação foi realizada usando o algoritmo de máxima verossimilhança (MLA) adaptado ao algoritmo STRS. Trinta segmentos de 30x30 km foram escolhidos ao longo das principais regiões agrícolas do estado, com dados da safra de verão de 2005/2006 (outubro a março), e mapeados com base em dados de campo e de imagens orbitais TM/Landsat-5 e CCD/CBERS-2. Cinco classes temáticas foram consideradas: Soja, Floresta, Cerrado, Pastagem e Solos Expostos. A classificação pelo método das STRS foi feita com base em uma área interseccionada por um subconjunto de segmentos de 30x30 km. O STRS superestimou os valores de referência em 21,31% em regiões com predomínio da cultura da soja e em 132,37% em regiões nas quais a soja era menos predominante. A exatidão global da classificação foi de 80%. As imagens MODIS e o algoritmo STRS mostraram-se promissores para a classificação da soja em regiões com predominância de grandes fazendas. Entretanto, os resultados para áreas fragmentadas em fazendas menores foram menos eficientes, superestimando as áreas de soja

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Factors Related to Textbook Outcome in Laparoscopic Liver Resections: a Single Western Centre Analysis

Introduction The selection of the most informative quality of care indicator for laparoscopic liver surgery (LLS) is still debated; among those proposed, textbook outcome (TO) seems to provide a compositive measure of the outcomes of surgery. The aim of this study was to investigate the factors related with the TO in a cohort of patients who underwent LLS. Methods Patients who underwent LLS from 2014 to 2021 were included. TO for LLS (TOLLS) was defined as: R0 resection, absence of intraoperative incidents, severe complications, reintervention, 30-day readmission and in-hospital mortality. When also considering no prolonged length of hospital stay (LOS), the outcome was called TOLLS+. Results Four hundred twenty-one patients were included; TOLLS was achieved in 80.5%, TOLLS+ in 60.8% cases. R0 resection was obtained in 90.2% cases, intraoperative incidents occurred in 7.8%, severe complications in 5.0%, reintervention in 0.7%, readmission in 1.4% and in-hospital mortality in 0.2%. 32.5% of patients showed prolonged LOS. After univariate and multivariate analysis, factors influencing TOLLS were age (OR 0.967; p=0.003), concomitant surgery (OR 0.380; p=0.003), operative time (OR 0.996; p=0.008) and blood loss (OR 0.241; p<0.001); factors influencing TOLLS+ were ASA-score (OR 0.533; p=0.008), tumour histology (OR 0.421; p=0.021), concomitant surgery (OR 0.293; p<0.001), operative time (OR 0.997; p=0.016) and blood loss (OR 0.361; p=0.003). Conclusions TOLLS can be achieved in most patients undergoing LLR, and it seems to be influenced mostly by surgery-related factors; conversely, TOLLS+ is achieved less frequently and seems to be influenced also by patient- and tumour-related factors

Modis vegetation indices applied to soybean area discrimination

O objetivo deste trabalho foi avaliar o desempenho do índice de vegetação realçado (EVI) e do índice de vegetação da diferença normalizada (NDVI) – ambos do sensor “moderate resolution imaging spectroradiometer” (Modis) –, para discriminar áreas de soja das áreas de cana‑de‑açúcar, pastagem, cerrado e floresta, no Estado do Mato Grosso. Foram utilizadas imagens adquiridas em dois períodos: durante a entressafra e por ocasião do pleno desenvolvimento da cultura da soja. Para cada classe analisada, foram selecionadas 31 amostras de mapas de referência e avaliadas as diferenças nos valores de cada índice de vegetação, para a classe soja, foram avaliadas frente às demais classes, por meio do teste de Tukey‑Kramer. Em seguida, foram avaliadas as diferenças entre os índices de vegetação, por meio do teste de Wilcoxon pareado. O NDVI apresentou melhor desempenho na discriminação das áreas de soja na entressafra, particularmente com uso das imagens do dia do ano (DA) 161 a 273, enquanto o EVI apresentou melhor desempenho no período de pleno desenvolvimento da cultura, especificamente com uso das imagens de DA 353 a 33. Portanto, o melhor resultado para classificação da soja, no Estado do Mato Grosso, via séries temporais do sensor Modis, pode ser obtida por meio do uso combinado do NDVI na entresssafra e do EVI no pleno desenvolvimento da soja.The objective of this work was to evaluate the performance of the enhanced vegetation index (EVI) and the normalized difference vegetation index (NDVI) – both from the moderate resolution imaging spectroradiometer (Modis) sensor – to discriminate soybean cultivated areas from sugarcane, pasture, cerrado, and forest ones in the state of Mato Grosso, Brazil. Images acquired during two periods were used: off-season and maximum soybean crop development. For each analyzed class, 31 samples were selected from reference maps, and the differences in the values of each soybean vegetation index were evaluated against the other classes using the Tukey‑Kramer test. Afterwards, the differences between the vegetation indices were assessed using the Wilcoxon paired test. NDVI performed best in discriminating soybean areas during the off-season period, particularly when using images acquired from day of year (DOY) 161 to 273, whereas EVI performed best during maximum crop development, particularly when using images from DOY 353 to 33. Therefore, best classification results for soybean in the state of Mato Grosso can be achieved by coupling Modis NDVI images acquired during off-season period and EVI images acquired during the maximum crop development period