Development of a MALDI–TOF MS-Based Protein Fingerprint Database of Common Food Fish Allowing Fast and Reliable Identification of Fraud and Substitution

Fish substitution and fish fraud are widely observed in the global food market. To detect and prevent substitution, DNA-based methods do not always meet the demand of being time- and cost-efficient; therefore, methodology improvements are needed. The use of species-specific protein patterns, as determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry, has recently improved species identification of prokaryotes both time- and cost-wise. We used the method to establish a database containing protein patterns of common food fish prone to substitution. The database currently comprises 54 fish species. Aspects such as the sensitivity of identification on the species level and the impact of bacterial contamination of fish filets are assessed. Most database entries are characterized by low intraspecies but high interspecies variability. Hitherto, 118 validation samples were successfully determined. The results presented herein underline the potential and reliability of eukaryotic species identification via MALDI–TOF mass spectrometry

Ferroelectricity in Simple Binary ZrO₂ and HfO₂

The transition metal oxides ZrO₂ and HfO₂ as well as their solid solution are widely researched and, like most binary oxides, are expected to exhibit centrosymmetric crystal structure and therewith linear dielectric characteristics. For this reason, those oxides, even though successfully introduced into microelectronics, were never considered to be more than simple dielectrics possessing limited functionality. Here we report the discovery of a field-driven ferroelectric phase transition in pure, sub 10 nm ZrO₂ thin films and a composition- and temperature-dependent transition to a stable ferroelectric phase in the HfO₂–ZrO₂ mixed oxide. These unusual findings are attributed to a size-driven tetragonal to orthorhombic phase transition that in thin films, similar to the anticipated tetragonal to monoclinic transition, is lowered to room temperature. A structural investigation revealed the orthorhombic phase to be of space group <i>Pbc</i>2₁, whose noncentrosymmetric nature is deemed responsible for the spontaneous polarization in this novel, nanoscale ferroelectrics