6 research outputs found
Serum antibody responses after challenge of vaccinated sheep.
<p>Mean antibody isotype responses of sheep before vaccination (wk -8), 1 week after vaccination (wk -3), at day of challenge (wk 0) and 1 week after challenge (wk +1). Serum diluted 1/5000 (IgG1), 1/500 (IgG2), 1/20 (IgA) or 1/32 ammonium sulphate cut (IgE). For details of experimental groups, refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078357#pone-0078357-t001" target="_blank">Table 1</a>. Dashed line represents the mean of a separate group of 8 sheep kept in indoor pens for 2–3 months. *Significant difference between group 3 and control group 1.</p
Correlations between parasitological and immunological parameters in control and vaccinated sheep.
*<p>Spearman rank correlation coefficient (P value); bold numbers indicate significance at P<0.05. ND: not done.</p
Faecal egg counts and worm burden after challenge of vaccinated sheep.
<p>A: Mean (+SEM) daily eggs/gram faeces (EPG); B: Mean (+SEM) cumulative faecal egg count (FEC); C: individual and mean (+/−SEM) total worm counts. For details of experimental groups, refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078357#pone-0078357-t001" target="_blank">Table 1</a>. Significantly different from the control group at p<0.05 (*) or p<0.01 (**).</p
Sheep numbers, immunization protocols and levels of protection against <i>H.contortus</i> infection.
<p>ND: not done; *p<0.05, **p<0.001.</p
Packed cell volume (PCV) of peripheral blood after challenge of vaccinated sheep.
<p>Mean (+SEM) PCV of groups at 49 days post infection (DPI). The mean PCV of all sheep before infection (day 0) was 34.2 (SD 2.3). For details of experimental groups, refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078357#pone-0078357-t001" target="_blank">Table 1</a>.</p
Eosinophil infiltration after intradermal injection of saline or exsheathed L3 <i>H. contortus</i> larvae (xL3).
<p>Representative H&E stained sections of skin tissue injected with saline (a) or xL3 (b) of group 1,2&4 sheep. Group 3 sheep (Ag+DEAE-dextran) showed much higher eosinophil infiltration (c) and occasional granulomas (d).</p