Vorrichtung zur Aufnahme einer Fluessigkeit sowie Vorrichtung zur Aufbringung von Fluessigkeiten auf Probentraeger und Verfahren hierzu

DE 102007041071 A1 UPAB: 20090403 NOVELTY - The fluid reception device for supplying the fluid on sample carrier (8), comprises recesses (9), whose base has holes, which are formed so that the capillary pressure in the respective hole is greater than the pressure produced through the fluid in the respective recess. The hole has a diameter of 50 mu m and a length of 1200 mu m and/or 200 mu m. The device represents a multi-corrugated plate, which has 96, 384 or 1536 corrugations and represents a micro-corrugated plate (4). The corrugated base of the multi-corrugated plate has thin glass capillaries as the hole. DETAILED DESCRIPTION - The fluid reception device for supplying the fluid on sample carrier, comprises recesses (9), whose base has holes, which are formed so that the capillary pressure in the respective hole is greater than the pressure produced through the fluid in the respective recess. The hole has a diameter of 50 mu m and a length of 1200 mu m and/or 200 mu m. The device represents a multi-corrugated plate, which has 96, 384 or 1536 corrugations and represents a micro-corrugated plate (4). The corrugated base of the multi-corrugated plate has thin glass capillaries as the hole. The corrugated base of the multi-corrugated plate is formed from a glass plate or an adhesive or laminated plastic foil. The multi-corrugated plate is sealed towards the top through a flexible membrane. INDEPENDENT CLAIMS are included for: (1) a device for supplying the fluid on sample carrier; and (2) method for supplying the fluid on sample carrier. USE - Fluid reception device for supplying the fluid on sample carrier useful for the analysis of substance in biotechnology field. ADVANTAGE - The fluid reception device avoids cleaning and disinfection of sample container and ensures simple, safe and economical analysis of sample in accurate and time consuming manner by avoiding the danger of contamination of the sample

Das smarte Labor der Zukunft: Ein disruptiver Wandel

Das klassische Labor gehört bald der Vergangenheit an. Der Experimentierort zur handwerklichen Gewinnung von empirischen Daten hat ausgedient. Die Auswertung großer Mengen digitaler Daten übernimmt auch hier, langsam aber unaufhaltbar, die Funktion Wissen zu generieren

Flexible fabrication kit - flexible interlinking, design and improvement of automated lab processes

Nowadays new biological techniques like tissue engineering, human cell culturing or pharmaceutical applications like manufacturing of biochips are considered key technologies of the 21st century. Nevertheless, the design of completely automated processes is characterized by high investment costs and development risks. These issues are caused by many different process steps including liquid handling, monitoring, micro and macro handling of biological assays forming a lab process. In order to improve complex processes and to simplify the refitting between different applications a flexible interlinking of individual process steps is desired. The Fraunhofer IPA has developed a modular fabrication kit, the so called "FabKit", which already represents established lab process applications and different OEM components including liquid handling systems, micro dispensing systems, measuring sensors etc. These stand-alone process modules can be mounted onto a platform in an uncomplicated way, enabling the users to create a custom made production and processing system to suit their products, components and requirements. A flexible interlinking of the modules is realized by a planar servo motor. Also local climate control and cleaning systems are already included. A novel software tool kit supports virtual planning, configuration, simulation and scheduling of the "FabKit" system. The presentation will cover the technological aspects of a flexible fabrication kit for laboratory applications and will point out the innovative solutions. Furthermore it will discuss trends and future challenges of highly flexible systems for pharmaceutical and biotechnological applications

Immediate Drop on Demand Technology (I-DOT): Poster presented Forum Life Science 2015, 9th International Congress and Exhibition, 11-12 March 2015, Garching, Germany

The handling and dosing of cells is the most critical step in the microfabrication of 3D-cell-based assay systems for screening and toxicity testing applications. The use of robotic workstations for liquid handling has increased enormously over the last decades, in order to meet the requirements for high accuracy and high throughput of current bioanalytics. The immediate drop on demand technology (I-DOT) overcomes the challenge of dispensing cells in a small volume precisely and with low shear stress. I-DOT provides a fast and flexible non-contact liquid handling system enabling dispensing cells and liquid without the risk of cross-contamination to a precise volume even in nanoliter range

Linking imaging to omics utilizing image-guided tissue extraction

Phenotypic heterogeneity is commonly observed in diseased tissue, specifically in tumors. Multimodal imaging technologies can reveal tissue heterogeneity noninvasively in vivo, enabling imaging-based profiling of receptors, metabolism, morphology, or function on a macroscopic scale. In contrast, in vitro multiomics, immunohistochemistry, or histology techniques accurately characterize these heterogeneities in the cellular and subcellular scales in a more comprehensive but ex vivo manner. The complementary in vivo and ex vivo information would provide an enormous potential to better characterize a disease. However, this requires spatially accurate coregistration of these data by image-driven sampling as well as fast sample-preparation methods. Here, a unique image-guided milling machine and workflow for precise extraction of tissue samples from small laboratory animals or excised organs has been developed and evaluated. The samples can be delineated on tomographic images as volumes of interest and can be extracted with a spatial accuracy better than 0.25 mm. The samples remain cooled throughout the procedure to ensure metabolic stability, a precondition for accurate in vitro analysis