Additional file 3: of Effect of CYP2C9, VKORC1, and CYP4F2 polymorphisms on warfarin maintenance dose in children aged less than 18Â years: a protocol for systematic review and meta-analysis

A list of the eligibility information. (PDF 3601 kb

Additional file 2: of Effect of CYP2C9, VKORC1, and CYP4F2 polymorphisms on warfarin maintenance dose in children aged less than 18Â years: a protocol for systematic review and meta-analysis

Search terms and strategies. The search strategy utilized is outlined in more detail in the file. (DOCX 23 kb

Axially Chiral Macrocyclic <i>E</i>-Alkene Bearing Bisazole Component Formed by Sequential C–H Homocoupling and Ring-Closing Metathesis

Clipping by ring-closing metathesis freezes rotation of a C–C bond to result in forming axial chirality. Treatment of bisbenzimidazole bearing an <i>N</i>-(3-butenyl) substituent with a Grubbs’ catalyst undergoes ring-closing metathesis, in which the stereochemistry of the thus formed olefin was exclusively <i>E</i>-form. Analysis by HPLC with a chiral stationary column confirmed clear baseline separation of each enantiomer

Additional file 1: of Monoclonal antibody therapy for Kawasaki disease: a protocol for systematic reviews and meta-analysis

PRISMA-P checklist: recommended items to address in a systematic review protocol. (DOC 81 kb

Additional file 2: of Monoclonal antibody therapy for Kawasaki disease: a protocol for systematic reviews and meta-analysis

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Expression of tilapia CREBs during: (A) natural ovarian cycle and (B) hCG induced -oocyte maturation as determined by RT-PCR and Northern Blot respectively. A plasmid DNA with CREB partial cDNA was used as positive control (PC) and PCR reaction without RT was used as negative control (NC) (Ma, Marker). (C) Densitometric analysis of CREB2 and CREB3 (mean ±SEM of two independent experiments).

<p>Expression of tilapia CREBs during: (A) natural ovarian cycle and (B) hCG induced -oocyte maturation as determined by RT-PCR and Northern Blot respectively. A plasmid DNA with CREB partial cDNA was used as positive control (PC) and PCR reaction without RT was used as negative control (NC) (Ma, Marker). (C) Densitometric analysis of CREB2 and CREB3 (mean ±SEM of two independent experiments).</p

Clustal W multiple alignment of tilapia and catfish CREB deduced amino acid sequences.

<p>Clustal W multiple alignment of tilapia and catfish CREB deduced amino acid sequences.</p

Cladogram showing phylogenetic analysis of the Nile tilapia and catfish CREBs with other vertebrate analogs.

<p>(Accession no.: Human A <u>NM_004379</u>; Human B <u>NM_134442</u>; Mouse C <u>NM_001037726</u>; <i>Xenopus</i><u>NM_001086603</u>;Mouse A <u>NM_009952</u>; Mouse B <u>NM_133828</u>; <u>Rat A NM_ 134443</u>; Rat B <u>NM_031017</u>; Chicken <u>NM_ 204450</u>; Zebrafish <u>NM_ 200909</u>; Pig <u>NM_001099929</u>; Cattle <u>NM_174285</u>; Songbird <u>NM_001048256</u>).</p

Northern blot analysis of CREBs.

<p><b>(A)</b> Expression of CREBs in tilapia ovary and testis <b>(</b>VOF; Vitellogenic Ovarian Follicle; FIO, Full grown Immature Ovarian Follicle; MOF, Mature Ovarian follicle). <b>(B)</b> Representative densitometric analysis of CREB2 and CREB3 of tilapia. Bars depicting same letter are significantly different from each other (mean ±SEM of two independent experiments). <b>(C)</b> Northern blot analysis of CREBs catfish ovary <b>(</b>P, Preparatory; PS, Pre-spawning; S, Spawning). <i>Note</i>: only one form of CREB (~ 1.3 kb) in tilapia testis [CREB 1] while two forms of CREB (~2.85 [CREB 2] and ~2.75 kb [CREB 3]) in tilapia ovary.</p

Tissue distribution pattern of tilapia CREBs as determined by semi-quantitative RT-PCR.

<p>A plasmid DNA of CREB partial cDNA was used as positive control (PC) and PCR reaction without RT was used as negative control (NC). (Ma, Marker; B, Brain; A, Adrenal; H, Heart; S, Spleen; L, Lung; I, Intestine; K, Kidney; M, Muscle; T, Testis; O, Ovary; F, Ovarian follicle).</p