The role of Toll-like receptor-4 in pertussis vaccine-induced immunity-2

Uvant (C), twice before intranasal infection. Two hours before challenge, and three and seven days after challenge mice were weighed. Data are indicated as mean ± SEM (N = 6). Non-boxed -values: compared to the adjuvant control (same strain and day after challenge). Boxed -value: compared to the wild-type strain (same treatment and day after challenge). ANOVA followed by Bonferroni post-hoc test. A single representative experiment of 2 is shown.<p><b>Copyright information:</b></p><p>Taken from "The role of Toll-like receptor-4 in pertussis vaccine-induced immunity"</p><p>http://www.biomedcentral.com/1471-2172/9/21</p><p>BMC Immunology 2008;9():21-21.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2409298.</p><p></p

The role of Toll-like receptor-4 in pertussis vaccine-induced immunity-0

Injected with 1/5 human dose (HD) wP, aP, or adjuvant (C), twice before intranasal infection. Three and seven days after challenge lungs were excised, and the number of viable was determined in right lung lobes. Each symbol represents the number of bacteria in the lung of an individual mouse; horizontal lines represent the group average. Non-boxed -values: compared to the indicated treatment group (same strain and day after infection). Boxed -values: compared to the wild-type strain (same treatment and day after infection). ANOVA followed by Bonferroni post-hoc test. A single representative experiment of 2 is shown.<p><b>Copyright information:</b></p><p>Taken from "The role of Toll-like receptor-4 in pertussis vaccine-induced immunity"</p><p>http://www.biomedcentral.com/1471-2172/9/21</p><p>BMC Immunology 2008;9():21-21.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2409298.</p><p></p

The role of Toll-like receptor-4 in pertussis vaccine-induced immunity-1

Uvant (C), twice before intranasal infection. Two hours before challenge, and three and seven days after challenge blood was taken. Test and positive control sera were tested for Ptx-, FHA-, and Prn-specific IgG. Each symbol represents the serum level of an individual mouse; horizontal lines represent the group average. (#) increased compared to the adjuvant control (same strain and time point; < 0.001), (+) increased compared to wP-vaccinated mice (same strain and time point; < 0.001). Non-boxed -values: compared to the indicated group (same strain and time point), boxed -values: compared to the wild-type strain (same treatment and time point), and stippled-boxed P-values: compared to the pre-challenge level (same strain and treatment). ANOVA followed by Bonferroni post-hoc test. A single representative experiment of 2 is shown.<p><b>Copyright information:</b></p><p>Taken from "The role of Toll-like receptor-4 in pertussis vaccine-induced immunity"</p><p>http://www.biomedcentral.com/1471-2172/9/21</p><p>BMC Immunology 2008;9():21-21.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2409298.</p><p></p

The role of Toll-like receptor-4 in pertussis vaccine-induced immunity-5

Injected with 1/5 human dose (HD) wP, aP, or adjuvant (C), twice before intranasal infection. Three and seven days after challenge lungs were excised, and the number of viable was determined in right lung lobes. Each symbol represents the number of bacteria in the lung of an individual mouse; horizontal lines represent the group average. Non-boxed -values: compared to the indicated treatment group (same strain and day after infection). Boxed -values: compared to the wild-type strain (same treatment and day after infection). ANOVA followed by Bonferroni post-hoc test. A single representative experiment of 2 is shown.<p><b>Copyright information:</b></p><p>Taken from "The role of Toll-like receptor-4 in pertussis vaccine-induced immunity"</p><p>http://www.biomedcentral.com/1471-2172/9/21</p><p>BMC Immunology 2008;9():21-21.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2409298.</p><p></p

Comparative gene expression profiling in two congenic mouse strains following infection-3

<p><b>Copyright information:</b></p><p>Taken from "Comparative gene expression profiling in two congenic mouse strains following infection"</p><p>http://www.biomedcentral.com/1471-2180/7/88</p><p>BMC Microbiology 2007;7():88-88.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2174938.</p><p></p>-producing glands. Lung lesions were scored semi-quantitatively as absent (0), minimal (1), slight (2), moderate (3), marked (4), or severe (5) per type of lesion and added up to calculate the pathology-score (range 0–15). Dots represent the pathology score per individual mouse, horizontal lines represent the groups median

Comparative gene expression profiling in two congenic mouse strains following infection-5

<p><b>Copyright information:</b></p><p>Taken from "Comparative gene expression profiling in two congenic mouse strains following infection"</p><p>http://www.biomedcentral.com/1471-2180/7/88</p><p>BMC Microbiology 2007;7():88-88.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2174938.</p><p></p> or down-regulated at an FDR < 0.05. Relatively increased gene-expression is presented in red while relatively decreased gene-expression is presented in green. The color scale shows log(2) ratios of gene-expression levels compared to the global average. ANOVA analysis identified 206 genes that were differentially expressed between the two mouse strains and 2,353 genes that were differentially expressed after infection. Hierarchical clustering of the 2,559 regulated genes results in four distinct groups, higher in C3H (A), higher in HcB-28 (B), down-regulated after infection in both strains (C) and up-regulated after infection in both strains (D)

Comparative gene expression profiling in two congenic mouse strains following infection-0

<p><b>Copyright information:</b></p><p>Taken from "Comparative gene expression profiling in two congenic mouse strains following infection"</p><p>http://www.biomedcentral.com/1471-2180/7/88</p><p>BMC Microbiology 2007;7():88-88.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2174938.</p><p></p> or down-regulated at an FDR < 0.05. Relatively increased gene-expression is presented in red while relatively decreased gene-expression is presented in green. The color scale shows log(2) ratios of gene-expression levels compared to the global average. ANOVA analysis identified 206 genes that were differentially expressed between the two mouse strains and 2,353 genes that were differentially expressed after infection. Hierarchical clustering of the 2,559 regulated genes results in four distinct groups, higher in C3H (A), higher in HcB-28 (B), down-regulated after infection in both strains (C) and up-regulated after infection in both strains (D)

Comparative gene expression profiling in two congenic mouse strains following infection-4

<p><b>Copyright information:</b></p><p>Taken from "Comparative gene expression profiling in two congenic mouse strains following infection"</p><p>http://www.biomedcentral.com/1471-2180/7/88</p><p>BMC Microbiology 2007;7():88-88.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2174938.</p><p></p>on scale from 1–5), as seen by inflammatory cells in alveolar septa and spaces. C3H day 3, alveolitis and thickened septa (pneumonia) varying from grade 3 (upper, right) to 5 (bottom). C3H day 3, asterix: perivasculitis grade 5, and arrow: hypertrophy bronchiolar epithelium, grade 3. HcB28 day 3, no major differences in pathology compared to C3H (B and C)

Comparative gene expression profiling in two congenic mouse strains following infection-2

<p><b>Copyright information:</b></p><p>Taken from "Comparative gene expression profiling in two congenic mouse strains following infection"</p><p>http://www.biomedcentral.com/1471-2180/7/88</p><p>BMC Microbiology 2007;7():88-88.</p><p>Published online 12 Oct 2007</p><p>PMCID:PMC2174938.</p><p></p> body weights were measured daily