Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence-7

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p>cterial cfu for the wild-type (clear columns), Δ0928 (black columns) and 0928C (grey columns) strains expressed as a proportion of the inoculum after incubation in human blood for 4 h. < 0.01 () for the Δ0928 strain versus the wild-type or 0928C strains

Immunoblots using antibodies to lipoproteins (PsaA, PiuA, PiaA, SlrA and PpmA) and pneumolysin (Ply) to probe whole-cell lysates and the lipid and aqueous phases of Triton X-114 extracts of wild-type, Δ0928 and 0928C bacteria

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> Sizes of lipoproteins were approximately 1–2 kDa larger in the Δ0928 strain, and in the wild-type strain were similar to the purified recombinant lipoprotein without the N-terminal signal peptide sequence for PiuA and PiaA (data not shown)

Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence-8

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p

Immunoelectron microscopy of thin sections of the wild-type and Δ0928 strains using antibodies to the PpmA and SlrA lipoproteins

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> Controls with bacteria stained with the secondary antibody alone demonstrated only one or two gold particles per cell (not shown), and controls probed with anti-Ply are included to demonstrate the pattern of protein expression for an intracellular protein. The scale bar in the top right panel is 0.2 μm long

Coomassie stained SDS-PAGE of Triton X-114 extracts from wild-type, 0928C and Δ0928 strains

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> Numbered lines correspond to the band numbers shown in , and the gels are aligned so that the migration positions of molecular mass markers (marked for the Δ0928 strain gel) are identical between the three gels

Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence-4

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p

Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence-0

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p

Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence-9

<p><b>Copyright information:</b></p><p>Taken from "Maturation of lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence"</p><p></p><p>Molecular Microbiology 2007;67(3):541-557.</p><p>Published online 16 Dec 2007</p><p>PMCID:PMC2228790.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p

Expression of a Cu,Zn superoxide dismutase typical for familial amyotrophic lateral sclerosis increases the vulnerability of neuroblastoma cells to infectious injury-2

<p><b>Copyright information:</b></p><p>Taken from "Expression of a Cu,Zn superoxide dismutase typical for familial amyotrophic lateral sclerosis increases the vulnerability of neuroblastoma cells to infectious injury"</p><p>http://www.biomedcentral.com/1471-2334/7/131</p><p>BMC Infectious Diseases 2007;7():131-131.</p><p>Published online 12 Nov 2007</p><p>PMCID:PMC2211486.</p><p></p

Construction of the Δ<i>lgt</i> strain.

<p>(A) Schematic of the Sp_1410–1413 locus, with the TIGR4 genome gene number and the assigned gene names in parentheses when available. Arrows indicate transcriptional direction and <i>lgt</i> is shaded black. (B) Structure of the Sp_1410–1413 locus in the Δ<i>lgt</i> mutant strain, showing replacement of <i>lgt</i> with an in-frame copy of <i>cat</i> which is shaded grey and position of primers used in (C). (C) Gel red stained agarose gels showing PCR analysis of two separately obtained Δ<i>lgt</i> strains confirming replacement of <i>lgt</i> with <i>cat</i>. Primer pairs (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041393#pone-0041393-t004" target="_blank">Table 4</a>) and the strain used for each reaction are given above each lane. (D) Gel red stained agarose gels of RT-PCR reactions using internal primers (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041393#pone-0041393-t004" target="_blank">Table 4</a>) for each gene within the putative Sp_1410–1413 operon, confirming the non-polar deletion of <i>lgt</i>. Reactions not containing reverse transcriptase gave no products (not shown), and L marks the DNA ladder size marker with sizes listed on the left.</p