Male longevity responses of four <i>Drosophila</i> species in response to four dietary treatments.

Male longevity responses of four Drosophila species in response to four dietary treatments.</p

Survival curves of four <i>Drosophila</i> species in response to dead Baker’s yeast.

Female and male lifespan of D. mel., D. gut., D. def., and D. tri. treated with diets including no yeast (blue), minimum dead yeast (1 scoop/fly/day, red), and minimum alive yeast (1 scoop/fly/day, green). (TIF)</p

Survival curves of <i>D</i>. <i>mel</i>. on a medium diet with and without rapamycin.

Survival curves for male and female lifespan in [days] for D. mel. on medium (2 scoops/fly/per day) diets with (blue) and without (red) rapamycin (200 μM). n = number of flies going into the analysis; escaped or flies killed by accident were discarded. (TIF)</p

Longevity respsonses of <i>D</i>. <i>mel</i>. to medium diet with and without rapamycin.

Longevity respsonses of D. mel. to medium diet with and without rapamycin.</p

Female fecundity in response to rapamycin in four <i>Drosophila</i> species on <i>ad libitum</i> diets.

The average egg-lay response across the first ten reproductive days of D. mel., D. gut., D. def., and D. tri. on an ad libitum diet, with or without the addition of rapamycin, are shown. A) D. mel. B) D. gut. C) D. def. D) D. tri. p<0.001: ‘***’, p< 0.01 = ‘**’.</p

Female fecundity in response to rapamycin in <i>D</i>. <i>mel</i>. on a medium diet.

The average egg-lay response over the first 10 reproductive days of D. mel. treated with a medium yeast diet (2 scoops/fly/day) with or without rapamycin. p<0.05: ‘*’.</p

Survival curves of four <i>Drosophila</i> species in response to four dietary treatments.

Survival curves for D. mel., D. gut., D. def., and D. tri. treated with diets ranging from no yeast (purple), minimum (1 scoop/fly/day, blue), to ad libitum (red), as well as an ad libitum yeast diet on rapamycin-treated medium (200 μM, green). Escaped or flies killed by accident were discarded. (TIF)</p

Female longevity responses of four <i>Drosophila</i> species in response to four dietary treatments.

Female longevity responses of four Drosophila species in response to four dietary treatments.</p

Suvival curve Log-Rank results.

p-values for all compared survival curves regarding longevity are shown. (XLSX)</p

Ovary dissections of <i>D</i>. <i>mel</i>. on four different diets.

All images were taken at 25X magnification. The white bars measure 0.5 mm. A) D. mel. ovaries on a medium diet (2 scoops/fly/day). B) ovaries on an ad libitum yeast diet. C) ovaries on a medium diet with rapamycin. D) ovaries on an ad libitum yeast diet with rapamycin.</p