2 research outputs found
Efficient Magnetic Recycling of Covalently Attached Enzymes on Carbon-Coated Metallic Nanomagnets
In the pursuit of robust and reusable
biocatalysts for industrial
synthetic chemistry, nanobiotechnology is currently taking a significant
part. Recently, enzymes have been immobilized on different nanoscaffold
supports. Carbon coated metallic nanoparticles were found to be a
practically useful support for enzyme immobilization due to their
large surface area, high magnetic saturation, and manipulatable surface
chemistry. In this study carbon coated cobalt nanoparticles were chemically
functionalized (diazonium chemistry), activated for bioconjugation
(<i>N,N</i>-disuccinimidyl carbonate), and subsequently
used in enzyme immobilization. Three enzymes, β-glucosidase,
α-chymotrypsin, and lipase B were successfully covalently immobilized
on the magnetic nonsupport. The enzyme–particle conjugates
formed retained their activity and stability after immobilization
and were efficiently recycled from milliliter to liter scales in short
recycle times
Efficient Magnetic Recycling of Covalently Attached Enzymes on Carbon-Coated Metallic Nanomagnets
In the pursuit of robust and reusable
biocatalysts for industrial
synthetic chemistry, nanobiotechnology is currently taking a significant
part. Recently, enzymes have been immobilized on different nanoscaffold
supports. Carbon coated metallic nanoparticles were found to be a
practically useful support for enzyme immobilization due to their
large surface area, high magnetic saturation, and manipulatable surface
chemistry. In this study carbon coated cobalt nanoparticles were chemically
functionalized (diazonium chemistry), activated for bioconjugation
(<i>N,N</i>-disuccinimidyl carbonate), and subsequently
used in enzyme immobilization. Three enzymes, β-glucosidase,
α-chymotrypsin, and lipase B were successfully covalently immobilized
on the magnetic nonsupport. The enzyme–particle conjugates
formed retained their activity and stability after immobilization
and were efficiently recycled from milliliter to liter scales in short
recycle times