Dynamic remodelling of synapses can occur in the absence of the parent cell body-6

<p><b>Copyright information:</b></p><p>Taken from "Dynamic remodelling of synapses can occur in the absence of the parent cell body"</p><p>http://www.biomedcentral.com/1471-2202/8/79</p><p>BMC Neuroscience 2007;8():79-79.</p><p>Published online 26 Sep 2007</p><p>PMCID:PMC2048966.</p><p></p>urofilament 165 and SV2 (green: axons and terminals), visualised with a Cy2-conjugated secondary antibody and co-stained with TRITC-αBTX (red: muscle endplates), 165 min after a 15 min BzATP pulse (100 μM). Both 'occupied' (a: arrow) and 'unoccupied' (a: arrowhead) endplates were present, as were unoccupied endplates with intact presynaptic axons (b). Many terminals appeared to be in the process of retraction resulting in minor (c: asterisk) or major (d: asterisks) regions of unoccupied endplates. These were classified as 'intermediate' in subsequent data. Control nerve/muscle preparations, which were maintained in physiological saline for up to 300 min, showed no visible signs of degeneration or retraction of nerve terminals (e). Scale bar a-e = 15 μ

Dynamic remodelling of synapses can occur in the absence of the parent cell body-1

<p><b>Copyright information:</b></p><p>Taken from "Dynamic remodelling of synapses can occur in the absence of the parent cell body"</p><p>http://www.biomedcentral.com/1471-2202/8/79</p><p>BMC Neuroscience 2007;8():79-79.</p><p>Published online 26 Sep 2007</p><p>PMCID:PMC2048966.</p><p></p>plates present in: 300 min control (open bar); 165 min subsequent to a 15 min BzATP pulse (100 μM: filled bar); 165 min subsequent to a 15 min BzATP pulse (filled bar) in the presence of Brilliant Blue G (a selective P2X7 receptor antagonist: BBG, 1 μM: broad diagonal cross-hatching); 165 min subsequent to a 15 min BzATP pulse in the presence of Reactive Blue 2 (which blocks P2Y and P2X receptor subunits: 100 μM, RB2: narrow diagonal cross-hatching) or 165 min subsequent to a 15 min BzATP pulse in the presence of Suramin (a broad spectrum P2 receptor agonist: 100 μM: stipple). All data is mean ± SEM of multiple repeats (see text for n values)

Dynamic remodelling of synapses can occur in the absence of the parent cell body-2

<p><b>Copyright information:</b></p><p>Taken from "Dynamic remodelling of synapses can occur in the absence of the parent cell body"</p><p>http://www.biomedcentral.com/1471-2202/8/79</p><p>BMC Neuroscience 2007;8():79-79.</p><p>Published online 26 Sep 2007</p><p>PMCID:PMC2048966.</p><p></p>urofilament 165 and SV2 (green: axons and terminals), visualised with a Cy2-conjugated secondary antibody and co-stained with TRITC-αBTX (red: muscle endplates), 165 min after a 15 min BzATP (100 μM) pulse. Nerve terminals associated with either fragmented (a), or absent (b) muscle endplates were seen, as were fragmented nerve terminals contacting either apparently normal (c), or fragmented endplates (c, d). Scale bar a, d = 15 μm, b, c = 30 μ

Dynamic remodelling of synapses can occur in the absence of the parent cell body-8

<p><b>Copyright information:</b></p><p>Taken from "Dynamic remodelling of synapses can occur in the absence of the parent cell body"</p><p>http://www.biomedcentral.com/1471-2202/8/79</p><p>BMC Neuroscience 2007;8():79-79.</p><p>Published online 26 Sep 2007</p><p>PMCID:PMC2048966.</p><p></p>urofilament 165 and SV2 (green: axons and terminals), visualised with a Cy2-conjugated secondary antibody and co-stained with TRITC-αBTX (red: muscle endplates), 165 min after a 15 min BzATP (100 μM) pulse. Nerve terminals associated with either fragmented (a), or absent (b) muscle endplates were seen, as were fragmented nerve terminals contacting either apparently normal (c), or fragmented endplates (c, d). Scale bar a, d = 15 μm, b, c = 30 μ

Dynamic remodelling of synapses can occur in the absence of the parent cell body-7

<p><b>Copyright information:</b></p><p>Taken from "Dynamic remodelling of synapses can occur in the absence of the parent cell body"</p><p>http://www.biomedcentral.com/1471-2202/8/79</p><p>BMC Neuroscience 2007;8():79-79.</p><p>Published online 26 Sep 2007</p><p>PMCID:PMC2048966.</p><p></p>plates present in: 300 min control (open bar); 165 min subsequent to a 15 min BzATP pulse (100 μM: filled bar); 165 min subsequent to a 15 min BzATP pulse (filled bar) in the presence of Brilliant Blue G (a selective P2X7 receptor antagonist: BBG, 1 μM: broad diagonal cross-hatching); 165 min subsequent to a 15 min BzATP pulse in the presence of Reactive Blue 2 (which blocks P2Y and P2X receptor subunits: 100 μM, RB2: narrow diagonal cross-hatching) or 165 min subsequent to a 15 min BzATP pulse in the presence of Suramin (a broad spectrum P2 receptor agonist: 100 μM: stipple). All data is mean ± SEM of multiple repeats (see text for n values)