Transformation of maize with the p1 transcription factor directs production of silk maysin, a corn earworm resistance factor, in concordance with a hierarchy of floral organ pigmentation

The maize p1 gene encodes an R2R3-MYB transcription factor that controls the biosynthesis of red flavonoid pigments in floral tissues of the maize plant. Genetic and quantitative trait locus analyses have also associated the p1 gene with the synthesis of maysin, a flavone glycoside from maize silks that confers natural resistance to corn earworm. Here, we show directly that the p1gene induces maysin accumulation in silk tissues. Transformation of maize plants that had low or no silk maysin with p1 transgenes elevated silk maysin concentrations to levels sufficient for corn earworm abiosis. The p1 transgenes also conferred red pigment to pericarp, cob, husk and tassel tissues, as expected; however, different subsets of these tissues were pigmented within individual transgenic plants. Statistical analysis shows that the pigmentation patterns observed amongst the p1 transgenic plants conform to a hierarchy that is similar to the temporal ordering of floral organ initiation. We propose that the observed hierarchy of pigmentation patterns is conferred by variation due to epigenetic control of the p1 transgenes. The production of plants with improved traits through genetic engineering can depend in large part on the achievement of tight organ-specific expression of the introduced transgenes. Our results demonstrate that the production of transgenic plants using a promoter with well-defined tissue specificity, such as the p1 promoter, can result in unexpected variation in tissue specificity amongst the resulting transgenic plants

Developing a Global Culture of Collaboration for School Counselors

School-university partnerships are becoming increasingly popular within the field of school counseling. Peer consultation groups offer a specific type of partnership and support for practitioners, numerous advantages for those in higher education, and significant potential for international application. This article expands on Thomas’ (2005) peer consultation model developed for school counseling alumni, with adaptations of the model in two universities, as well as several K-12 school districts within a single county. Data from participant evaluations indicate strong support for the personal and professional value of peer consultation

The developmental vitamin D (DVD) model of schizophrenia

It is now widely acknowledged that exposure to adverse environmental factors in utero may not only affect how the brain develops but have long-lasting consequences for later brain function in the adult offspring. This idea has gained particular prominence amongst researchers interested in the etiology of neurodevelopmental disorders such as schizophrenia and autism. Approximately 10 years ago we proposed that developmental vitamin D (DVD) deficiency may explain several epidemiological features of this disease, most noticeably the winter/spring season of birth effect. In 2003 we published results from our first study indicating there were structural changes in how the brain develops in these offspring. Since then we have firmly established that DVD deficiency not only affects brain cell differentiation and gross anatomy but also produces alterations in behavior in these offspring as adults. In this chapter we describe how we came to construct the model we use today. Over the past 7 years the model has proved informative producing both structural brain changes (ventriculomegaly) and behavioral alterations (hyperlocomotion in response to NMDA antagonists) that are thought to be relevant to schizophrenia

Chemical analysis on the honey of Heterotrigona itama and Tetrigona binghami from Sarawak, Malaysia

This study aims to compare the chemical composition of honey samples produced by Heterotrigona itama and Tetrigona binghami which originated from Sarawak, Malaysia. One hundred and six (106) honey samples were collected from local bee farms and analysed in terms of their chemical profiles. The chemical analysis conducted includes physicochemical composition such as moisture, total phenolic content, sugar, 5-hydroxymethylfurfural (5-HMF), pH and organic acids and proximate analysis which included ash, protein, carbohydrates and energy. Independent T-test was used as a statistical tool to investigate the significant difference between the composition of both honey samples. The results showed that honey samples of Heterotrigona itama and Tetrigona binghami possessed significant difference (p<0.05) in moisture, total phenolic content, fructose, glucose, pH, protein, gluconic acid, acetic acid, ash, carbohydrates and energy. The honey samples of Heterotrigona itama exhibited significantly higher fructose and glucose at the average of 22.00 ± 3.48 g/ 100 g and 23.45 ± 3.23 g/100 g, respectively. Besides, the honey samples also possessed higher pH value, gluconic acid, ash, carbohydrates and energy. Meanwhile, Tetrigona binghami honey samples possessed significantly (p< 0.05) higher moisture content, total phenolic content, protein and acetic acid compared to the Heterotrigona itama’s honey samples. To conclude, the geographical and floral origins of honey are the two important quality parameters which fundamentally affect the physical-chemical properties as well as biological activities of honey samples

Cognitive Impairment, Frailty, and Adverse Outcomes Among Prevalent Hemodialysis Recipients:Results From a Large Prospective Cohort Study in the United Kingdom

RATIONALE &amp; OBJECTIVE: Frailty and cognitive impairment are common in hemodialysis recipients and have been associated with high mortality. There is considerable heterogeneity in frailty reporting, with little comparison between commonly used frailty tools and little exploration of the interplay between cognition and frailty. The aims were to explore the relationship between frailty scores and cognition and their associations with hospitalization and mortality.STUDY DESIGN: Prospective cohort study.SETTING &amp; POPULATION: Prevalent hemodialysis recipients linked to national datasets for hospitalization and mortality.PREDICTORS: Montreal Cognitive Assessment (MoCA), Frailty Phenotype, Frailty Index (FI), Edmonton Frailty Scale, and Clinical Frailty Scale (CFS) were performed at baseline. Cognitive impairment was defined as MoCA scores of &lt;26, or &lt;21 in dexterity impairment, &lt;18 in visual impairment.OUTCOMES: Mortality, hospitalization.ANALYTICAL APPROACH: Cox proportional hazards model for mortality, censored for end of follow-up. Negative binomial regression for admission rates, censored for death/end of follow-up.RESULTS: In total, 448 participants were recruited with valid MoCAs and followed up for a median of 685 days. There were 103 (23%) deaths and 1,120 admissions of at least one night. Cognitive impairment was identified in 346 (77.2%) participants. Increasing frailty by all definitions was associated with poorer cognition. Cognition was not associated with mortality (HR, 0.99; 95% CI, 0.95-1.03; P = 0.41) or hospitalization (IRR, 1.01; 95% CI, 0.99-1.04; P = 0.39) on multivariable analyses. There were interactions between MoCA scores and increasing frailty by FI (P = 0.002) and Clinical Frailty Scale (P = 0.005); admissions were highest when both MoCA and frailty scores were high, and when both scores were low.LIMITATIONS: As frailty is a dynamic state, a single cross-sectional assessment may not accurately reflect its year-to-year variability. In addition, these findings are in maintenance dialysis and may not be transferable to incident hemodialysis. There were small variations in application of frailty tool criteria from other studies, which may have influenced the results.CONCLUSIONS: Cognitive impairment is highly prevalent in this hemodialysis cohort. The interaction between cognition and frailty on rates of admission suggests the MoCA offers value in identifying higher risk hemodialysis populations with both high and low degrees of frailty.</p

The effect of EMDR versus EMDR 2.0 on emotionality and vividness of aversive memories in a non-clinical sample

Background: Eye movement desensitization and reprocessing (EMDR) therapy is a treatment meant to reduce vividness and emotionality of distressing memories. There is accumulating evidence that working memory taxation is the core of the working mechanism of EMDR therapy and that EMDR derives its effect by taxing the working memory (WM) with a dual task while actively keeping a disturbing memory in mind. From a theoretical stance, based upon assumptions derived from the WM theory, the effectiveness of EMDR therapy could be improved by several adaptations. Objectives: To test the assumption that integrating these elements into the standard EMDR protocol would enhance EMDR therapy, this adapted version of EMDR (i.e. EMDR 2.0), was compared to standard EMDR in a laboratory setting. It was hypothesized that EMDR 2.0 would be more efficacious than standard EMDR, and show a greater decrease in emotionality and vividness than standard EMDR therapy. Our second hypothesis was that EMDR 2.0 would be more efficient than standard EMDR in that this variant needs less session time and a smaller number of sets (i.e. approximately 30 seconds of WM taxation). Method: Non-clinical participants (N = 62, 79% female, mean age = 35.21) with a disturbing autobiographical memory were randomly allocated to receive either EMDR or EMDR 2.0. Emotionality and vividness of the memory were measured pre- and post-intervention, and at 1- and 4-week follow-up. Results: The results showed no difference between EMDR and EMDR 2.0 in decreasing emotionality and vividness, and no difference in session time. However, participants in the EMDR 2.0 condition needed fewer sets than those in the standard EMDR condition. Conclusion: The notion that EMDR 2.0 is more efficient is partially supported by the results showing participants needed less sets than in standard EMDR to reach the same results. Future research with clinical samples is warranted