Additional file 1: Figure S1. of Methylomic markers of persistent childhood asthma: a longitudinal study of asthma-discordant monozygotic twins

Flow chart describing the methodological approach used in this study. Our analyses focused on identifying differentially methylated positions (DMPs) associated with asthma in (A) all asthma-discordant MZ twins at age 10 and (B) a sub-group with persistent asthma who were discordant for asthma at age 10 and also at age 18. Using DNA previously collected at age 5, we (C) subsequently assessed longitudinal changes in DNA methylation (between ages 5 and 10) in persistent-asthma-discordant MZ twins. Finally, we (D) examined epigenetic variation at top-ranked persistent-asthma-associated DMPs in an asthma-remission group, comprising of MZ twin pairs discordant for asthma at age 10 but concordant for no asthma phenotype at 18 and concordant unaffected MZ twin pairs where neither twin had asthma at both ages 10 and 18

Additional file 2: Tables S1–S7. of Methylomic markers of persistent childhood asthma: a longitudinal study of asthma-discordant monozygotic twins

Table S1. The top-ranked DMPs (P < 0.001) in discordant MZ twin pairs at age 10. Table S2. Gene ontology enrichment analysis for age-10 asthma-associated DMPs. Table S3. The top-ranked DMPs at age 10 in persistent-asthma-discordant MZ twins (between 10 and 18 years). Table S4. Gene ontology enrichment analysis for persistent-asthma age-10 DMPs. Table S5. The top-ranked CpG sites which show changes in DNA methylation levels between 5 and 10 years of age in the asthma-discordant MZ twins. Table S6. Gene ontology enrichment analysis of longitudinal DMPs between 5 and 10 years of age. Table S7. Monozygotic twin group details