The Caribou Québec Corporation: one step toward caribou conservation

The operation of Caribou Québec will be based on the general principle that the resource users (native and non-native people and communities, outfitters, sport hunters, commercial users, etc.) in collaboration with related government organizations, participate actively in conservation and management projects

Le climat psychologique des unités organisationnelles : élaboration et validation d'un questionnaire

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal

The Caribou Québec Corporation: one step toward caribou conservation

The operation of Caribou Québec will be based on the general principle that the resource users (native and non-native people and communities, outfitters, sport hunters, commercial users, etc.) in collaboration with related government organizations, participate actively in conservation and management projects

L’implantation d’un nouveau protocole sur la méthadone

Résumé Objectif : Faciliter les voies de communication lors du transfert d’un patient hospitalisé vers un autre milieu lorsque l’on a instauré un traitement à la méthadone à visée analgésique. Mise en contexte : Un médecin de l’équipe du soulagement de la douleur prescrit de la méthadone à son patient hospitalisé. Lorsque vient le moment de signer le congé, plusieurs scénarios peuvent se présenter : le médecin assume le suivi, un changement de médecin est assuré, le patient est transféré dans un autre milieu de soins. Les deux dernières situations impliquent la possibilité que le médecin de relais ne détienne pas l’exemption obligatoire pour prescrire la méthadone. Effective - ment, un médecin ne peut s’improviser prescripteur de méthadone sans obtenir au préalable une autorisation de prescrire de la méthadone (exemption) auprès de Santé Canada. Au Centre hospitalier universitaire de Sherbrooke, nous avons mis sur pied un outil afin que le transfert du patient dans un autre environnement se fasse le plus aisément possible. Conclusion : Ce projet se veut un moyen facilitant la diffusion de l’information. Il explique le bien-fondé de l’ordonnance, les façons de faire pour obtenir les autorisations, les caractéristiques particulières du médicament. Les propriétés thérapeutiques de la méthadone méritent d’être exploitées, et ce, même si sa prescription est réglementée. Abstract Objective: To facilitate communication between healthcare settings upon transfer of a hospitalized patient who has been initiated on methadone for analgesic purposes. Context: A physician from pain control service prescribes methadone to a hospitalized patient. Upon discharge of the patient, many scenarios are possible: the prescribing physician follows up with the patient, a different physician takes charge, or the patient is transferred to another healthcare setting. In the latter two of these situations, there is a possibility that the new physician in charge is not an authorized methadone prescriber. As it is, a physician cannot prescribe methadone without first obtaining authorization or an exemption from Health Canada. At the Centre Hospitalier Universitaire de Sherbrooke (CHUS), we have implemented a working tool to ensure that the transfer of patients to another setting is done as easily as possible. Conclusion: This project was meant to facilitate the diffusion of information. It justifies the prescription, it explains the steps to be taken to obtain authorization, and it explains the medication specific characteristics. Although methadone prescribing is regulated, methadone’s therapeutic properties should nonetheless be exploited. Key Words: cancer, pain, drug interactions, methadone, opiates, opiate rotation, Health Canada

Caspase-3 Regulates Catalytic Activity and Scaffolding Functions of the Protein Tyrosine Phosphatase PEST, a Novel Modulator of the Apoptotic Response

The protein tyrosine phosphatase PEST (PTP-PEST) is involved in the regulation of the actin cytoskeleton. Despite the emerging functions attributed to both PTPs and the actin cytoskeleton in apoptosis, the involvement of PTP-PEST in apoptotic cell death remains to be established. Using several cell-based assays, we showed that PTP-PEST participates in the regulation of apoptosis. As apoptosis progressed, a pool of PTP-PEST localized to the edge of retracting lamellipodia. Expression of PTP-PEST also sensitized cells to receptor-mediated apoptosis. Concertedly, specific degradation of PTP-PEST was observed during apoptosis. Pharmacological inhibitors, immunodepletion experiments, and in vitro cleavage assays identified caspase-3 as the primary regulator of PTP-PEST processing during apoptosis. Caspase-3 specifically cleaved PTP-PEST at the (549)DSPD motif and generated fragments, some of which displayed increased catalytic activity. Moreover, caspase-3 regulated PTP-PEST interactions with paxillin, leupaxin, Shc, and PSTPIP. PTP-PEST acted as a scaffolding molecule connecting PSTPIP to additional partners: paxillin, Shc, Csk, and activation of caspase-3 correlated with the modulation of the PTP-PEST adaptor function. In addition, cleavage of PTP-PEST facilitated cellular detachment during apoptosis. Together, our data demonstrate that PTP-PEST actively contributes to the cellular apoptotic response and reveal the importance of caspases as regulators of PTPs in apoptosis