85 research outputs found
Somatostatin Is Expressed in FRTL-5 Thyroid Cells and Prevents Thyrotropin-Mediated Down-Regulation of the Cyclin-Dependent Kinase Inhibitor p27kip11
9 pages, 8 figures, 3 tables.Using RT and amplification, we have detected specific RNA transcripts encoding somatostatin in FRTL-5 thyroid cells. This observation indicates that within the thyroid context, expression of somatostatin is not restricted to the parafollicular C cells. Transfection of FRTL-5 cells with constructs containing either the complete somatostatin gene promoter or deletions carrying the cAMP response element-binding site allowed us to demonstrate that transcription of the somatostatin gene is hormonally regulated by TSH. Blockage of somatostatin by specific antibodies resulted in an increased capacity of TSH-induced FRTL-5 cell-conditioned medium to promote cell proliferation, demonstrating that under physiological conditions, somatostatin exerts a cytostatic effect on FRTL-5 cells growth. Somatostatin treatment of FRTL-5 cells resulted in a growth retardation, caused by a dose-response delay in the G1 phase of the cell cycle. This effect appears to be mediated by the cyclin-dependent kinase inhibitor p27kip1, which is clearly down-regulated in FRTL-5 cells treated with TSH and whose expression is reestablished by somatostatin in a dose-dependent manner. Participation of somatostatin in the control of FRTL-5 cell proliferation is in agreement with the detection of specific somatostatin receptor type 2. Flow cytometric assays reveal that FRTL-5 cells transformed with the K-ras oncogene are still sensitive to somatostatin treatment, whereas fully neoplastic FRT cells no longer respond to this peptide. Taking together, the results demonstrate the participation of an autocrine loop in the control of thyroid cell proliferation, and the possibility that this mechanism could be altered in the process of thyroid carcinogenesis.This work was supported by Grants DGICYT (PM97–0065), CAM
(08.1/0025/1997), and Fundación Salud 2000 (Spain).Peer reviewe
Ocular surface stem cells and disease: Current concepts and clinical applications
Annals of the Academy of Medicine Singapore335576-580AAMS
Modified automated lamellar therapeutic keratoplasty for keratoconus: A new technique
10.1097/01.ico.0000248388.39767.42Cornea25101217-1219CORN
Automated lamellar therapeutic keratoplasty for post-PRK corneal scarring and thinning
10.1016/j.ajo.2004.06.062American Journal of Ophthalmology13861067-1069AJOP
Recurrent corneal erosion after laser in situ keratomileusis
10.1097/00003226-200103000-00008Cornea202156-158CORN
Tectonic corneal lamellar grafting for severe scleral melting after pterygium surgery
10.1016/S0161-6420(03)00260-4Ophthalmology11061126-1136OPHT
Ocular surface changes in pterygium
10.1097/00003226-200201000-00009Cornea21138-42CORN
Accurate intraocular pressure measurement in contact lens wearers with normal pressures
CLAO Journal232130-133CLAJ
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