DODAVANJE NUKLEOTIDA ELIMINIRA OŠTEĆENJE LEUKOCITNE DNA IZAZVANE - T-2 TOKSINOM I DEOXYNIVALENOLOM U BROJLERSKIH PILIĆA

This study shows that Fusarium mycotoxins, T-2 toxin and DON impair the performance of broiler chickens, induce DNA damage in chicken leukocytes and cause oxidative stress in the organism. When considering feed consumption and live weight gain in mycotoxicosis induced with high levels of T-2 toxin and DON present in the feed, dietary nucleotides do not have beneficial effect. The crucial role of nucleotide supplementation in feed is to repair DNA damage in immune cells, which are highly sensitive to mycotoxin action. When broilers are exposed to Fussarium mycotoxins their immune function can be depressed, thus they can be more susceptible to virus or bacterial infections. The addition of dietary nucleotides may increase proliferation of immune cells and optimize the function of the immune system in case of infections.Ovaj rad pokazuje da mikotoksini Fusarium, toksini T-2 i DON kvare proizvodne rezultate u brojlera, izazivaju oštećenje DNK u leukocitima pilića i prouzrokuju oksidacijski stres u organizmu. S obzirom na konzumaciju hrane i prirast žive vage u mikotoksikozi izazvanoj visokim razinama toksina T-2 i DON-a što se nalaze u hrani, dijetalni nukleotidi nemaju blagotvorno djelovanje. Presudna uloga dodavanja nukleotida u hranu je obnoviti gubitak DNK u imunim stanicama koje su vrlo osjetljive na djelovanje mikotoksina. Kad su brojleri izloženi mikotoksinima Fusarium njihova se imuna funkcija može smanjiti pa mogu biti osjetljiviji na virusne ili bakterijske infekcije. Dodavanje dijetalnih nukleotida može povećati razmnožavanje imunih stanica i optimirati funkciju imunog sustava u slučaju infekcije

Effect of dietary linseed oil and Ganoderma lucidum or olive leaves supplementation on fatty acid composition and oxidative status of rabbits

[EN] The aim of the present study was to evaluate the influence of natural antioxidant supplementation on the oxidative stress induced by a high proportion of polyunsaturated fatty acids (PUFA) in rabbit diets. Forty-eight SIKA rabbits (24 male, 24 female) were penned individually and after a 5-d adaptation period divided into 4 homogeneous groups (2.58±0.3 kg). The CONT– group received a diet with 60 g/kg palm fat, while the other 3 groups received a diet with 60 g/kg linseed oil which was either unsupplemented (CONT+) or supplemented with 10 g/kg of Ganoderma lucidum (REISHI) or 10 g/kg of olive leaves (OLIVE). Rabbits were euthanised at 102 d of age, 22 d after the start of the experiment. Live weight, weight gain, feed intake and feed conversion rate were recorded. The fatty acid composition of back (M. longissimus dorsi) and hind leg muscle (thigh muscle), adipose tissue (abdominal fat) and liver were determined. To evaluate the oxidative status of rabbits, the malondialdehyde (MDA) concentration in the plasma, liver and both muscles was measured. DNA damage in the leukocytes was measured. Linseed oil addition increased the PUFA ratio and decreased the proportion of saturated fatty acids in all tissues (P<0.001), with no detrimental effect on productive performance of rabbits compared to palm fat. Linseed oil also reduced n-6/n-3 PUFA ratio in all tissues (P<0.001). However, linseed oil addition increased the oxidative stress by increasing MDA concentrations in the liver and muscles (P<0.001), but did not affect the plasma MDA concentrations and the extent of DNA damage in the blood leukocytes compared to palm fat. Addition of olive leaves tended to decrease the concentration of MDA in the liver Addition of olive leaves tended to decrease the concentration of MDA in the liver compared to CONT+ (P=0.059), with no effect of Ganoderma lucidum.Trebušak, T.; Levart, A.; Frankič, T.; Salobir, J.; Pirman, T. (2014). Effect of dietary linseed oil and Ganoderma lucidum or olive leaves supplementation on fatty acid composition and oxidative status of rabbits. World Rabbit Science. 22(1):71-81. doi:10.4995/wrs.2014.1183SWORD718122