14 research outputs found
The effects of somatic mutations on EGFR interaction with anti-EGFR monoclonal antibodies: Implication for acquired resistance
Advantage of using a home-made elisa kit for detection of <i> Helicobacter pylori</i> infection over commercially imported kits
Advantage of using a home-made elisa kit for detection of Helicobacter pylori infection over commercially imported kits
Purpose: To evaluate a home-made ELISA kit for detection of
Helicobacter pylori (Hp) infection and comparison of its immunologic
criteria with those of foreign commercial kits. Methods: A home-made
IgG ELISA kit was developed using soluble antigenic fractions of Hp
proteins. Confirmed sera were tested and serological criteria were
evaluated through assessment of 199 serum samples. Results: The
accuracy, sensitivity and specificity values of home-made kit were 92,
92 and 90.4%, respectively. These immunologic criteria for Trinity kit
were 95.2, 95.2 and 95% in comparison with IBL kit (91.3, 92.2 and
88.5%), BIOHIT kit (72.4, 41.6 and 94.1%) and HelicoBlot2.1 (94.2, 93.4
and 100%). Kappa agreement assessment demonstrated that two of the
imported ELISA kits had fair to moderate agreement with the home-made
kit while the other one had a poor agreement value. Conclusions: Apart
from comparable values between the home-made kit and the most efficient
imported kit (Trinity) there was significant cost benefit. Therefore,
we recommend the home-made kit as a suitable substitution for detection
of Hp infection in the Iranian population
Relationship Between Helicobacter pylori BabA and BabB Status with Other Virulence Factors and Their Correlation with Disease Outcome in Iran
Construction of bacterial ghosts for transfer and expression of a chimeric hepatitis C virus gene in macrophages
Congenital craniofacial and skeletal defects with arthrogryposis in two newborn male Holstein Friesian calves
Oral Immunization with Recombinant Mycobacterium smegmatis Expressing the Outer Membrane Protein 26-Kilodalton Antigen Confers Prophylactic Protection against Helicobacter pylori Infection ▿ †
Helicobacter pylori infection is prevalent worldwide and results in chronic gastritis, which may lead to gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer. We have previously reported that oral immunization with recombinant Mycobacterium smegmatis expressing the H. pylori outer membrane protein 26-kilodalton (Omp26) antigen affords therapeutic protection against H. pylori infection in mice. In the present study, we investigated the prophylactic effects of this vaccine candidate on H. pylori challenge in mice. We found that oral immunization with recombinant Mycobacterium Omp26 significantly reduced H. pylori colonization in the stomach compared to inoculation with wild-type M. smegmatis in control mice. Six of the recombinant Mycobacterium-immunized mice (60%) were completely protected from H. pylori infection. The severity of H. pylori-associated chronic gastritis assessed histologically was significantly milder in mice vaccinated with recombinant Mycobacterium than in control animals. Mice immunized with recombinant Mycobacterium showed enhanced antigen-specific lymphocyte proliferation and antibody responses. Moreover, immunization with recombinant Mycobacterium resulted in an increased expression of interleukin-2 and gamma interferon in the stomach and spleen, as determined by reverse transcription-PCR analysis. Our results collectively suggest that vaccination with recombinant Mycobacterium Omp26 confers prophylactic protection against H. pylori infection. The inhibition of H. pylori colonization is associated with the induction of antigen-specific humoral and cell-mediated immune responses