2 research outputs found
Nocatriones A and B, Photoprotective Tetracenediones from a Marine-Derived <i>Nocardiopsis</i> sp.
Two new tetracenedione derivatives,
nocatriones A (<b>1</b>) and B (<b>2</b>), were discovered
from the culture broth
of a marine actinomycete, <i>Nocardiopsis</i> sp. KMF-002,
which was isolated from the tissue of an unidentified dark purple
marine sponge. The structures of <b>1</b> and <b>2</b>, which are tetracenediones containing α-pyrone substituents,
were determined to be 3,8,10,11-tetrahydroxy-2-(4-hydroxy-2-oxo-2<i>H</i>-pyran-6-yl)-1-methyltetracene-5,12-dione (<b>1</b>) and 3,8,10,12-tetrahydroxy-2-(4-hydroxy-2-oxo-2<i>H</i>-pyran-6-yl)-1-methyltetracene-6,11-dione (<b>2</b>). Ultraviolet
B (UVB)-irradiated cells treated with 10 μM nocatrione A (<b>1</b>) significantly decreased the level of MMP-1, a protein that
degrades collagen and other extracelluar matrix components that comprise
dermal tissue, when compared to untreated cells. These results support
that nocatriones A (<b>1</b>) and B (<b>2</b>) may show
antiphotoaging activity in UVB-irradiated models
Protective Effects of Processed Ginseng and Its Active Ginsenosides on Cisplatin-Induced Nephrotoxicity: <i>In Vitro</i> and <i>in Vivo</i> Studies
Although
cisplatin can dramatically improve the survival rate in cancer patients,
its use is limited by its nephrotoxicity. Previous investigations
showed that Panax ginseng contains
components that exhibit protective activity against cisplatin-induced
nephropathy. The aim of the present study is to investigate the effect
of microwave-assisted processing on the protective effect of ginseng
and identify ginsenosides that are active against cisplatin-induced
kidney damage to evaluate the potential of using ginseng in the management
of nephrotoxicity. The LLC-PK1 cell damage by cisplatin was significantly
decreased by treatment with microwave-processed ginseng (MG) and ginsenosides
Rg3, Rg5, and Rk1. Reduced expression of p53 and c-Jun N-terminal
kinase proteins by cisplatin in LLC-PK1 cells was markedly ameliorated
after Rg3 and Rg5/Rk1 treatment. Additionally, elevated expression
of cleaved caspase-3 was significantly reduced by ginsenosides Rg5,
Rk1, and with even greater potency, Rg3. Moreover, MG and its fraction
containing active ginsenosides showed protective effects against cisplatin-induced
nephropathy in mice. We found that ginsenosides Rg3, Rg5, and Rk1
generated during the heat treatment of ginseng ameliorate renal damage
by regulating inflammation and apoptosis. Results of current experiments
provide evidence of the renoprotective effects and therapeutic potential
of MG and its active ginsenosides, both <i>in vitro</i> and <i>in vivo</i>