MOESM3 of Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds

Additional file 3: Figure S1. RT-qPCR values of central metabolism and regulatory genes. Relative mRNA concentrations of central metabolism and regulatory genes of PB12 and PB13 strains, grown in glucose as the sole carbon source were determined by RT-qPCR. The PB12 values have been previously reported [2, 6, 7] and are presented here for discussion and comparison purposes. Data in this Figure are reported as relative expression levels of the parental strain JM101. The mRNA level of each gene in the parental strain was used as control to normalize the data, assigning it the value of one (see Materials and Methods)

MOESM2 of Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds

Additional file 2: Table S2. Mutation rates in both evolved strains based on mutation appearance in the rpoB locus. Mutation rates were determined by a modified Luria-Delbrück fluctuation test, employing the MSS-MLE and the LC methods in the estimation of the number of mutants [30–32]. The mutation frequency also was determinated