High throughput detection of by real-time PCR with internal control system and automated DNA preparation-1

the y-axis. "+" in "cell isolation" means isolation success as confirmed by detection of inclusion bodies upon microscopy. B, box plot analysis of threshold cycle values in real-time PCR positive/conventional negative (n = 32) and real-time PCR positive/conventional PCR positive (n = 38) samples. Difference in threshold cycle values are significant (p < 0.05).<p><b>Copyright information:</b></p><p>Taken from "High throughput detection of by real-time PCR with internal control system and automated DNA preparation"</p><p>http://www.biomedcentral.com/1471-2180/8/77</p><p>BMC Microbiology 2008;8():77-77.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2397412.</p><p></p

High throughput detection of by real-time PCR with internal control system and automated DNA preparation-2

Lood (x-axis). A, Qiagen DNA mini kit; B, Qiagen M48 DNA mini kit, used on a Qiagen M48 automated DNA extraction instrument. Each datum point represents the rate of positive results in six replicate tests per concentration. Limits of detection are comparable with both methods of DNA extraction. C, Threshold cycles (y-axis) as a measure of efficiency of PCR amplification for and internal control. Each reaction contained 15 copies of plasmid-derived target gene and variable numbers of internal control plasmid pCoxmimic, as depicted on the x-axis. Results of eight replicate real-time PCR reactions per setting are shown as a result of box-plot analysis, showing the range of results by whiskers, whereby the two central quartiles of data are represented as a box. Solid line with grey boxes, target gene, broken line with white boxes, internal control. No reduced efficiency in amplification is observed for the target gene in presence of up to 100 copies of internal control. D, Correlation of DNA copies per ml as determined by real-time PCR after automated (x-axis) and manual extraction procedure (y-axis).<p><b>Copyright information:</b></p><p>Taken from "High throughput detection of by real-time PCR with internal control system and automated DNA preparation"</p><p>http://www.biomedcentral.com/1471-2180/8/77</p><p>BMC Microbiology 2008;8():77-77.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2397412.</p><p></p