Additional file 4: of Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma

Table S2. Significant differentially expressed genes between normal cortical microglia, DIPG-associated macrophages, and aGBM-associated macrophages. (XLSX 75 kb

Additional file 1: of Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma

Table S1. Patient characteristics of early post-mortem DIPG autopsy cases. (XLSX 55 kb

Additional file 6: of Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma

Figure S4. DIPG cells do not express significant levels of cytokines (a-b) FPKMs of cytokine (left), chemokine (middle) and other factors (right) expressed by patient-derived DIPG cell cultures (a) or in bulk primary DIPG tissue (b) Horizontal line represents FPKM = 5 (c) Violin plots of single-cell DIPG expression of cytokines, chemokines, and other factors from primary DIPG biopsy tissue. Horizontal line represents log(tpm + 1) = 1. (TIF 1442 kb

Additional file 3: of Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma

Figure S2. Isolated microglia/macrophages are enriched for myeloid genes. FPKMs binned across sample type for isolated DIPG (blue), aGBM (red), and pediatric cortical microglia/macrophages (green). There is minimal or absent expression of genes associated with other major cortical cell types (astrocytes, neurons, OPCs, oligodendrocytes, and endothelial cells). (TIF 948 kb

Additional file 2: of Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma

Figure S1. Primary DIPG samples do not consistently demonstrate differential CD45 high/low populations (a-b) Representative FACS plots of primary DIPG tissue samples showing an example of an indistinguishable CD45 high/low sample (a) and a distinguishable CD45 high/low population (b). Samples were gated for size, singularity, and viability prior to these plots. (TIF 585 kb