To evaluate the anti-inflammatory activity of ramipril in albino rats

Background: Angiotensin II (Ang II) is a product of Renin angiotensin aldosterone system (RAAS). Angiotensin‑II regulates vascular tone, stimulates the release of pro‑inflammatory cytokines, activates nuclear factor‑kappa B (NF‑κB), increases oxidant stress and functions as an inflammatory molecule. Ramipril an ACE inhibitor act by inhibiting angiotensin converting enzyme, decreases angiotensinogen II activity. Hence the present was to evaluate the anti-inflammatory activity of Ramipril.Methods: Eighteen Wistar albino rats weighing around 150-200gms of either sex were randomly selected from central animal facility and divided into three groups. The control group received normal saline 25ml/kg, standard group received Indomethacin 10mg/kg and test group received Ramipril (0.9mg/kg) orally for six days. The animals were subjected to carrageenan induced paw oedema and cotton pellet induced granuloma model.Results: Ramipril significantly decreased the mean paw oedema in carrageenan induced paw oedema when compared to control and in cotton pellet induced granuloma Ramipril decreased the mean granuloma weight when compared to control.Conclusions: Ramipril showed anti-inflammatory activity when given for 6 consecutive days per orally in albino rats in carrageenan induced paw oedema and cotton pellet induced granuloma model

To evaluate the effect of neostigmine on blood glucose levels in euglycemic albino rats through OGTT

Background: Diabetes mellitus (DM) consists of a group of syndromes characterised by hyperglycaemia, altered metabolism of lipids, carbohydrates and proteins and an increased risk of complications from vascular disease. There are genetic and environmental components that affect the risk of developing either type 1 or type 2 diabetes mellitus.Methods: Twelve Swiss albino rats weighing around 150-200gmsof either sex were randomly selected from the central animal facility, JSSMC, Mysore and divided into two groups. The control group received distilled water (25ml/kg body wt.) per orally, test group received Neostigmine (0.5mg/kg/day) per orally for 5 days. On the fifth day, following overnight fasting, 1 hour after drug administration in all the group of rats OGTT was performed, by administering oral glucose in dose of 0.6gm/kg body weight. The capillary blood glucose level was measured at 0, 60 and 150 minutes, by rat tail snipping method using (ACCUCHEK) glucometer.Results: The Capillary Blood Glucose levels of Neostigmine group was less when compared to control group at all-time intervals.Conclusions: Neostigmine showed the hypoglycemic activity when given for 5 days orally in euglycemic albino rats through OGTT

Evaluation of an anti-diuretic activity of fluvoxamine in albino rats

Background: Diabetes insipidus is a disease characterized by high amounts of urine excretion. Antidiuretic drugs are used to treat this condition. Hence, our study intends to evaluate the anti-diuretic effect of fluvoxamine, a selective serotonin reuptake inhibitors in albino rats.Methods: Albino rats were divided into three groups of six animals each. The control group was fed with distilled water 10 ml/kg body weight, standard group received 4 units of vasopressin and test group received fluvoxamine 18 mg/kg body weight. On the day of experiment, diuresis was induced in all the groups by giving frusemide in a dose of 20 mg/kg body weight after loading with saline at 25 ml/kg body weight. The animals were confined in diuretic cage for a period of 5 hrs and urine output was noted. Urine was analyzed for electrolyte concentration (Na+, K+, Cl−).Results: There was significant reduction in urine output in the test group of animals when compared to the control group. Electrolyte concentration revealed relatively concentrated urine when compared to the control group.Conclusions: Fluvoxamine has a significant anti-diuretic action in the albino rats

Evaluation of analgesic activity of irbesartan in albino mice

Background: The objective was to evaluate the analgesic activity of irbesartan in albino mice.Methods: Swiss albino mice weighing 25-30 g of either sex were selected for the study. Six animals were allocated to each experimental group. The control group received normal saline (25 ml/kg, p.o.), standard group received pentazocine (10mg/kg, intraperitonial [i.p.]) and test group received irbesartan (20 mg/kg, p.o.). The above drugs were administered 1 hr prior to the experiments. In case of visceral pain model 0.6% acetic acid was given i.p. 30 mins prior to the experiment to induce writhing, in thermal pain model pretreated mice were placed on Eddy’s Hotplate maintained at 55°C and in mechanical stimulus pain model an artery clip was clamped at the base of the tail of pretreated mice. Decrease in total number of writhes in acetic acid induced writhing model and delay in reaction time in both Eddy’s hot plate and Tail clip method denoted analgesic activity respectively.Results: The test drug significantly decreased the total number of writhes in acetic acid induced writhing model in mice. The percentage inhibition of writhing was significant which was 84.35% in the standard group and 59.24% in the test group. The test drug significantly delayed the reaction time in both Eddy’s hot plate and tail clip method when compared to control group and standard group. Percentage increase in latency period when compared to standard drug was significant and measured 73.11% and 64.31% at 60 min in both Eddy’s hot plate and tail clip method, respectively.Conclusion: Irbesartan exhibits analgesic activity in albino mice

Study of the oral hypoglycemic activity of Moringaoleifera leaves alone and in combination with Glibenclamide in streptozotocin induced diabetic albino rats

Background: Oringaoleifera is a widely used plant with high medicinal value, well known for its pharmacological actions and is used in various conditions. It has been reported to have many biological properties like anti-inflammatory, antimicrobial, antispasmodic, antitumour including antidiabetic activity.Methods: The study was carried out in Wistar albino rats with body weight 150-250gms. Diabetes was induced by injecting Streptozotocin intraperitoneally- dose 55 mg/kg BW. Animals were divided into 5 groups with 6 animals in each group. First group (Control) was given 2% gum acacia. Other 4 groups were induced diabetes by giving Streptozotocin. Diabetic control group received gum acacia (0.5 ml), Standard group received Glibenclamide (0.5mg/kg BW), Test group received Moringaoleifera extract (300mg/kg) and Test+ Standard group receiving combination of Moringaoleifera and glibenclamide at half the above doses. All drugs were given orally for 28 days and blood glucose levels analyzed using Glucometer on Day 0 before drug and on D1, D3, D7, D14, D21, and D28. Data were statistically analyzed by ANOVA and Tukey‘s Post Hoc test.Results: Hypoglycemia produced by Moringaoleifera extract was significant (p<0.001) when compared to diabetic control group from day 7 to day 28. The percent reduction of blood glucose level was 52.9% as compared to Glibenclamide group 61.3%. The combination group also showed significant hypoglycemic activity the percentage reduction being 56.44%.Conclusions: Thus, Moringaoleifera decreased blood glucose level efficaciously as compared to diabetic control group and similar to standard group at p<0.001

Whole genome analysis and functional characterization of a novel Bacillus thuringiensis (Bt 62) isolate against sugarcane white grub Holotrichia serrata (F)

In this study, we report the whole genome assembly of Bt 62, a novel isolate harbouring cry8 holotype gene identified by us earlier. Sequencing was carried out using a combination of Illumina NextSeq 500 and Oxford Nanopore sequencing Technologies (ONT). The final assembled genome was 6.13 Mb comprising a circular chromosome and four plasmids. The bioassay studies against Holotrichia serrata (F.) (Coleoptera: Scarabaeidae), a polyphagous pest infesting sugarcane and other crops, indicated significant toxicity to first instar grubs over untreated larvae achieving a highest mean mortality of 91.11% for various doses tested. In vitro proteolytic assay and histopathological studies of the midgut of infected white grubs revealed proteolytic processing of the protoxin and extensive degeneration of larval midgut epithelial cells. The results demonstrate that this novel isolate could be used as a biopesticide or its crystal toxin genes could be expressed in sugarcane and other crops for resistance against H. serrata

To evaluate the anti-inflammatory activity of ramipril in albino rats

Background: Angiotensin II (Ang II) is a product of Renin angiotensin aldosterone system (RAAS). Angiotensin‑II regulates vascular tone, stimulates the release of pro‑inflammatory cytokines, activates nuclear factor‑kappa B (NF‑κB), increases oxidant stress and functions as an inflammatory molecule. Ramipril an ACE inhibitor act by inhibiting angiotensin converting enzyme, decreases angiotensinogen II activity. Hence the present was to evaluate the anti-inflammatory activity of Ramipril.Methods: Eighteen Wistar albino rats weighing around 150-200gms of either sex were randomly selected from central animal facility and divided into three groups. The control group received normal saline 25ml/kg, standard group received Indomethacin 10mg/kg and test group received Ramipril (0.9mg/kg) orally for six days. The animals were subjected to carrageenan induced paw oedema and cotton pellet induced granuloma model.Results: Ramipril significantly decreased the mean paw oedema in carrageenan induced paw oedema when compared to control and in cotton pellet induced granuloma Ramipril decreased the mean granuloma weight when compared to control.Conclusions: Ramipril showed anti-inflammatory activity when given for 6 consecutive days per orally in albino rats in carrageenan induced paw oedema and cotton pellet induced granuloma model

Evaluate the effect of bethanechol on blood glucose levels in euglycemic Wistar albino rats through oral glucose tolerance test

Objective: To evaluate the effect of bethanechol on blood glucose levels in euglycemic Wistar albino rats through oral glucose tolerance test (OGTT). Materials and Methods: Twelve Wistar albino rats weighing around 150-200 g of either sex were randomly selected from the central animal facility, and were divided into two groups. The control group received distilled water (25 mL/kg body wt.) per orally, test groups received bethanechol (3.6 mg/kg/day) per orally for 5 days. On the fifth day, following overnight fasting, 1 h after drug administration in all the groups of rats OGTT was performed, by administering oral glucose in dose of 0.6 gm/kg body weight. The capillary blood glucose (CBG) levels were measured at 0 min, 60 min, and 150 min, by rat tail snipping method using glucometer (ACCUCHEK). Results: The CBG levels of the bethanechol group was less when compared to the control group at all time intervals and the difference was statistically significant. Conclusion: Bethanechol showed the hypoglycemic activity when given for 5 days orally to euglycemic albino rats through OGTT