Additional file 4: Figure S4. of Statins induce insulin-degrading enzyme secretion from astrocytes via an autophagy-based unconventional secretory pathway

Cell death was not induced in this study. (A) MTS assay was used for checking cell viability under simvastatin, 3MA and/or bafilomycin treated condition. N = 5 experiments. (PDF 57 kb

Additional file 1: Figure S1. of Statins induce insulin-degrading enzyme secretion from astrocytes via an autophagy-based unconventional secretory pathway

Statins regulate cholesterol levels in astrocytes. (A) Cellular cholesterol levels were measured by filipin staining. MβCD is a positive control. (B) Quantitative analysis of Figure S1A using the Image J program (N = 3 experiments). ** p < 0.01, *** p < 0.001 vs. vehicle-treated cells. (PDF 217 kb

Additional file 2: Figure S2. of Statins induce insulin-degrading enzyme secretion from astrocytes via an autophagy-based unconventional secretory pathway

Fluvastatin induces IDE secretion from astrocytes. (A) Increased IDE levels secreted from the primary astrocytes by fluvastatin in a concentration-dependent manner. Blots are representative of at least 3 independent experiments (N = 3 experiments). (B) Quantitative analysis of Figure S2A. ** p < 0.01 vs. vehicle-treated cells. (PDF 71 kb

Additional file 1: Figure S1. of The novel RAGE interactor PRAK is associated with autophagy signaling in Alzheimer’s disease pathogenesis

Interaction between endogenous PRAK and RAGE. SH-SY5Y cell lysates were immunoprecipitated with RAGE antibody or normal IgG antibody. Western blot analysis performed with the indicated antibodies. (TIFF 308 kb