Additional file 1: Table S1. of Construction of environmental risk score beyond standard linear models using machine learning methods: application to metal mixtures, oxidative stress and cardiovascular disease in NHANES

Supplemental tables and figures for the construction of environmental risk score beyond standard linear models using machine learning methods. (DOCX 1620 kb

Additional file 4: of Inhibition of neddylation facilitates cell migration through enhanced phosphorylation of caveolin-1 in PC3 and U373MG cells

Neddylation inhibition enhances the Src-mediated phosphorylation of caveolin-1. Scratch-based wound healing assays were performed for 24 h in PC3 (A) and U373MG (B) cells which were depleted of NEDD8 using siRNA #2 and si-control in the absence or presence 10 μM PP2 (top). The migration areas were calculated using ImageJ at just below. Proteins in cells lysates were analyzed by Western blotting (middle). The level of the phosphorylation of caveolin-1 was quantified based upon the relative level of β-tubulin (bottom). Each bar represents the means + standard deviation of results from three independent experiments. * denotes P < 0.05 between the indicated groups. Scale bar = 200 μm. (PPTX 21157 kb

MOESM1 of Molecular clock of HIV-1 envelope genes under early immune selection

Additional file 1. Mathematical models for HIV evolution under immune selection, Table S1 (The rate of HIV gene sequence diversification over 2 years from the first sample in 15 subjects), and Table S2 (Documented CD8+ T cell epitopes for statistically designated selection sites from 15 subjects)

Additional file 3: of Inhibition of neddylation facilitates cell migration through enhanced phosphorylation of caveolin-1 in PC3 and U373MG cells

Phosphorylated caveolin-1 is essential for MLN4924-induced cell migration. Scratch-based wound healing assays were performed for 24 h in PC3 (A) and U373MG (B) cells which were depleted of caveolin-1 using siRNA (#1 and #2, respectively) and si-control in the presence of MLN4924 (0.25 μM and 0.5 μM) or DMSO (top). The migration areas were calculated using ImageJ at just below. Proteins in cells lysates were analyzed by Western blotting (middle). The efficiency of the caveolin-1 knock-down and magnitude of the phosphorylation of caveolin-1 was quantified based upon the relative level of β-tubulin (bottom). Each bar represents the means + standard deviation of results from three independent experiments. * denotes P < 0.05 and n, s, does P > 0.05 between the indicated groups. Scale bar = 200 μm. (PPTX 12560 kb

Nanoscale Domain Imaging of All-Polymer Organic Solar Cells by Photo-Induced Force Microscopy

Rapid nanoscale imaging of the bulk heterojunction layer in organic solar cells is essential to the continued development of high-performance devices. Unfortunately, commonly used imaging techniques such as tunneling electron microscopy (TEM) and atomic force microscopy (AFM) suffer from significant drawbacks. For instance, assuming domain identity from phase contrast or topographical features can lead to inaccurate morphological conclusions. Here we demonstrate a technique known as photo-induced force microscopy (PiFM) for imaging organic solar cell bulk heterojunctions with nanoscale chemical specificity. PiFM is a relatively recent scanning probe microscopy technique that combines an AFM tip with a tunable infrared laser to induce a dipole for chemical imaging. Coupling the nanometer resolution of AFM with the chemical specificity of a tuned IR laser, we are able to spatially map the donor and acceptor domains in a model all-polymer bulk heterojunction with resolution approaching 10 nm. Domain size from PiFM images is compared to bulk-averaged results from resonant soft X-ray scattering, indicating excellent quantitative agreement. Further, we demonstrate that in our all-polymer system, the AFM topography, AFM phase, and PiFM show poor correlation, highlighting the need to move beyond standard AFM for morphology characterization of bulk heterojunctions

Additional file 1: of A comprehensive and scalable database search system for metaproteomics

This file contains supplementary figures, methods, and four supplementary tables: Table S1. Data sources used for generation of ComPIL database. Table S2. Adenovirus 5 proteins identified by a ComPIL search of a human HEK293 sample. Table S3. List of proteomes used for generation of the “46 proteomes” database. Table S4. Statistics summary of 3 technical replicates of 5 human fecal samples. (ZIP 1371 kb

Additional file 1: of The regional association between bronchiectasis and lung cancer in chest CT

Figure S1. Example CT images of (A) underlying bronchiectasis and newly diagnosed lung cancer existing in the same lobe and (B) secondary traction bronchiectasis caused by lung cancer. (ZIP 7247 kb

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Additional file 1: Figure S1. of Disrupted-in-schizophrenia 1 (DISC1) and Syntaphilin collaborate to modulate axonal mitochondrial anchoring

Multiple SNPH association regions of DISC1. (A) Co-immunoprecipitation of FLAG-mDISC1 fragments with mSNPH-Myc in HEK293 cells. Lysates were immunoprecipitated with anti-FLAG and subjected to anti-FLAG and anti-SNPH western blotting. (TIF 1174 kb

Additional file 1: Figure S1. of MYC and BCL2 overexpression is associated with a higher class of Memorial Sloan-Kettering Cancer Center prognostic model and poor clinical outcome in primary diffuse large B-cell lymphoma of the central nervous system

Correlation of MYC, BCL2 and BCL6 IHC score. Correlations between MYC and BCL2 (left upper), MYC and BCL6 (right upper), and BCL2 and BCL6 (lower) IHC score was compared using Spearman correlation test. (PPT 343 kb