Thomas Joshua Cooper: Refuge

Thomas Joshua Cooper, one of the most celebrated and distinctive landscape photographers working today, explores the Eastern United States with a special focus on the East End of Long Island and the Hudson River. Throughout his career, Thomas Joshua Cooper has focused on the landscape through the lens of historical and cultural geography and cartography. Working exclusively outdoors with an 1898 AGFA field camera, Cooper has established a unique aesthetic and philosophy. This collection features twenty images that Cooper made on the East End of Long Island and Shelter Island in 2016, juxtaposed with earlier works depicting sites along the Hudson River, Cape Cod, and Maine. Each photograph is accompanied by texts that provide eloquent historical and geographic context. A special section on Cooper's unique and painstaking artistic process offers readers an understanding of how Cooper researches, experiences, and captures the essence of the locations he photographs. Exhibition: Parrish Art Museum, Water Mill, NY, USA (05.05. - 28.07.2019)

NanoString Digital Molecular Profiling of Protein and microRNA in Rhabdomyosarcoma

Purpose: Rhabdomyosarcoma (RMS) exhibits a complex prognostic algorithm based on histologic, biologic and clinical parameters. The embryonal (ERMS) and spindle cell-sclerosing RMS (SRMS) histologic subtypes warrant further studies due to their heterogenous genetic background and variable clinical behavior. NanoString digital profiling methods have been previously highlighted as robust novel methods to detect protein and microRNA expression in several cancers but not in RMS. Methods/Patients: To identify prognostic biomarkers, we categorized 12 ERMS and SRMS tumor cases into adverse (n = 5) or favorable (n = 7) prognosis groups and analyzed their signaling pathways and microRNA profiles. The digital spatial profiling of protein and microRNA analysis was performed on formalin-fixed, paraffin-embedded (FFPE) tumor tissue using NanoString technology. Results: The detectable expression of several component members of the PI3K/AKT, MAPK and apoptosis signaling pathways was highlighted in RMS, including INPP4B, Pan-AKT, MET, Pan-RAS, EGFR, phospho-p90 RSK, p44/42 ERK1/2, BAD, BCL-XL, cleaved caspase-9, NF1, PARP and p53. Compared to cases with favorable prognosis, the adverse-prognosis tumor samples had significantly increased expression of INPP4B, which was confirmed with traditional immunohistochemistry. The analysis of microRNA profiles revealed that, out of 798 microRNAs assessed, 228 were overexpressed and 134 downregulated in the adverse prognosis group. Significant over-expression of oncogenic/tumor suppressor miR-3144-3p, miR-612, miR-302d-3p, miR-421, miR-548ar-5p and miR-548y (p < 0.05) was noted in the adverse prognosis group. Conclusion: This study highlights the utility of NanoString digital profiling methods in RMS, where it can detect distinct molecular signatures with the expression of signaling pathways and microRNAs from FFPE tumor tissue that may help identify prognostic biomarkers of interest. The overexpression of INPP4B and miR-3144-3p, miR-612, miR-302d-3p, miR-421, miR-548y and miR-548ar-5p may be associated with worse overall survival in ERMS and SRMS