Warts/Lats and Mats/Mob Maximum Likelihood trees.

<p>A) Warts/Lats vertebrate topology matches the species tree. Among invertebrates, these proteins should be sequentially joined to the vertebrate cluster and thus this part of the Warts/Lats tree deviates from the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1C</a>) shows one difference - rather than the hemichordate and echinoderm clustering together they are sequentially clustered with the large urochordate, cephalochordate and vertebrate group, an arrangement that better matches the species tree. B) Mats/Mob vertebrate topology matches the species tree except for difficulty resolving human, mouse and chicken Mob1a. Among invertebrates a cluster of the echinoderm and urochordate sequences is attached to the vertebrate cluster with the hemichordate and cephalochordates as outliers. The association of urochordates with vertebrates matches the species tree but the inclusion of echinoderms without hemichordates and cephalochordates does not. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1D</a>) shows one difference - the urochordate switches places with the hemichordate leading to an arrangement that better matches the species tree but with no confidence.</p

Hippo/Mst and Salvador Maximum Likelihood trees.

<p>Trees rooted with <i>D. melanogaster</i> are shown. Branches considered statistically weak (aLRT values <0.70) are colored in blue. Branch lengths are drawn to scale and correspond to the average number of amino acid changes per site as indicated by the scale bar. A) Hippo/Mst vertebrate topology matches the species tree. Among invertebrates the hemichordate strongly clusters with vertebrates while the echinoderm is an outlier. The overall Hippo/Mst tree matches the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1A</a>) shows one difference - the hemichordate moves out of the strong cluster with vertebrates to become an outlier like the echinoderm. B) Salvador vertebrate topology matches the species tree. Among invertebrates the urochordate clusters tightly with vertebrates while the echinoderm and cephalochordate are outliers. The overall Salvador tree matches the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1B</a>) shows one difference - the cephalochordate clusters outside the urochordate and vertebrate cluster with confidence.</p

Schematic of the Hippo kinase pathway in flies and humans.

<p>A) Left side. In the protostome <i>D. melanogaster</i>, the transmembrane atypical cadherin ligand Dachsous (Ds) activates the transmembrane atypical cadherin receptor Fat (Ft). Fat then inhibits Dachs (D) and activates Expanded (Ex). Inhibition of Dachs prevents it from destabilizing the Warts (Wts) serine-threonine kinase and activation of Expanded leads to increased kinase activity of Warts. Expanded accomplishes this via a complex containing Merlin and Kibra that facilitates phosphorylation of the Hippo (Hpo) serine-threonine kinase, the Warts kinase and their respective co-factors Salvador (Sav) and Mats. Warts then phosphorylates the transcription co-activator Yorkie (Yki). Phosphorylated Yorkie is bound by 14-3-3 proteins and sequestered in the cytoplasm. When not phosphorylated, Yorkie translocates to the nucleus, binds transcription factors such as Scalloped (Sd), and influences target gene expression leading to increased cell proliferation and decreased apoptosis. A subset of known target genes is shown. Right side. In the deuterostome <i>H. sapiens,</i> proteins are shown in the same color and subcellular location as their corresponding <i>D. melanogaster</i> proteins: Hippo is Mst1/2, Warts is Lats1/2, Mats is Mob1a/b, Yorkie is Yap/Taz, Scalloped is Tead1/2/3/4, Expanded is Frmd1/6, and Merlin is Nf2. The roles of mammalian homologs of Dachsous, Fat, Expanded and Dachs have not yet been confirmed and they are shown with dashed lines and no color. Mammalian target genes are not homologs of the fly target genes. B) Formal names of the nine coelomate species in this study and their phylum/subphylum classifications.</p

Hippo Pathway Phylogenetics Predicts Monoubiquitylation of Salvador and Merlin/Nf2

<div><p>Recently we employed phylogenetics to predict that the cellular interpretation of TGF-β signals is modulated by monoubiquitylation cycles affecting the Smad4 signal transducer/tumor suppressor. This prediction was subsequently validated by experiments in flies, frogs and mammalian cells. Here we apply a phylogenetic approach to the Hippo pathway and predict that two of its signal transducers, Salvador and Merlin/Nf2 (also a tumor suppressor) are regulated by monoubiquitylation. This regulatory mechanism does not lead to protein degradation but instead serves as a highly efficient “off/on” switch when the protein is subsequently deubiquitylated. Overall, our study shows that the creative application of phylogenetics can predict new roles for pathway components and new mechanisms for regulating intercellular signaling pathways.</p> </div

Yorkie/Yap/Taz and Scalloped/Tead Maximum Likelihood trees.

<p>A) Yorkie/Yap/Taz (Taz is formally known as Wwtr1) vertebrate topology matches the species tree. Among invertebrates there is strong pairing of the echinoderm and hemichordate but weak connections of this pair to cephalochordates, urochordates and vertebrates in relationships that deviate from the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1E</a>) shows one difference - the urochordate has switched places with the cephalochordate exaggerating the deviation from the species tree. B) Scalloped/Tead vertebrate topology matches the species tree. Among invertebrates, the tree clusters the hemichordate and echinoderm with vertebrate Tead1 without the urochordate and clusters the urochordate with vertebrate Tead3, both are deviations from the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1F</a>) shows one difference - the echinoderm and hemichordate move away from vertebrate Tead1 as outliers, an arrangement that is a slightly better match to the species tree.</p

Overall amino acid substitution rates for families in the Wnt, Hippo and TGF-ß pathways.

a<p>Substitution rate per residue per billion years shown in ascending order by pathway. For each pathway an example of two physically interacting proteins with concordant rates is <u>underlined</u> (e.g., Shaggy/Gsk3 and Axin) and an example of two physically interacting proteins with discordant rates is <b>bold</b> (e.g., Fz and Arrow/Lrp).</p

Kibra/Wwc, Expanded/Frmd and Merlin/Nf2 Maximum Likelihood trees.

<p>A) Kibra/Wwc vertebrate topology matches the species tree. Among invertebrates there is a significant cluster of the echinoderm and urochordate that excludes the cephalochordate, a deviation from the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1G</a>) shows no differences. B) Expanded/Frmd vertebrate topology matches the species tree. <i>M. musculus</i> Frmd1 was found (NP_001191849.1) but excluded since it contains a mutation in the coding region that led it to be classified as a pseudogene. Our data showing this protein is well conserved in humans and chicken suggests that the mutation is a sequence error or belongs to a mutant allele rather than a pseudogene. Among invertebrates the cephalochordate strongly clusters with vertebrates and the echinoderm is an outlier matching the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1H</a>) shows no differences. C) Merlin/Nf2 vertebrate topology matches the species tree. Among invertebrates, the hemichordate, cephalochordate and echinoderm form a strong cluster without the urochordate, an arrangement that deviates from the species tree. The Bayesian tree (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051599#pone.0051599.s001" target="_blank">Figure S1I</a>) shows no differences.</p

Supplemental Material for Tran et al., 2018

Supporting information for Figures 5 and 6.<br

Supplemental Material for Tran et al., 2018

Table S1. <i>dCORL</i> mutant virgin and mated adult longevity defects compared to seven control lines.<div><br></div><div>Table S2. Mean & median longevity of <i>dCORL</i> virgin and mated adults with seven controls.<br></div><div><br></div><div>Table S3. Significant brain size reduction is present in <i>Df(4)dCORL</i> larvae but not adults.<br></div