Extensive homologies between lectins from non-leguminous plants

AbstractSequence studies were performed on lectins from two non-leguminous plants: rice and nettle. Extensive homologies were found between these two proteins and wheat germ agglutinin in support of the conservation of lectin sequences among non-leguminous plants. The number and positions of the cysteine residues were particularly well conserved suggesting a similar folding of the polypeptide chains

Visualization of cholinoceptive neurons in the rat neocortex:Colocalization of muscarinic and nicotinic acetylcholine receptors

The present investigation analyzes the cellular distribution of muscarinic and nicotinic acetylcholine receptors in rat neocortex, by use of monoclonal antibodies raised against purified receptor proteins. The degree of colocalization of both types of receptors was determined by way of immunofluorescent double-labeling techniques. For both classes of receptors, pyramidal and nonpyramidal cells were found immunostained and an identical laminar distribution pattern of immunopositive neurons in the rat neocortex became apparent. A striking similarity in distribution of the two cholinergic receptor types was found in the frontal/motor and parietal cortex. Accordingly, we observed a high degree of colocalization of muscarinic and nicotinic acetylcholine receptors within immunopositive cortical neurons. Approximately 90% of the cholinoceptive neurons expressed both types of receptors. The current data demonstrate that (i) the distribution of muscarinic and nicotinic cholinoceptive neurons in the neocortex is present in identical laminar patterns and represent the same type of cells, (ii) both classes of cholinergic receptors are highly colocalized within cholinoceptive neurons, which points at individual neurons as a likely site of interaction between muscarinic and nicotinic acetylcholine receptor-mediated processes.</p

Linkage in mice of genes controlling an immunoglobulin kappa-chain marker and the surface alloantigen Ly-3 on T lymphocytes

Evidence obtained using recombinant inbred and congenic mouse strains has shown that the PC8 locus responsible for determining a marker on a single k chain in inbred mice is linked to the Ly - 2,3 locus on chromosome 6. The upper limit of the map distance between these loci is approximately three centimorgans. This finding is discussed in relation to other known light-chain variants that are associated with the Ly - 2,3 locus.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46753/1/251_2005_Article_BF01563929.pd

Caracterisation moleculaire et fonctionnelle des marqueurs de membranes des cellules lymphoides de lapin

SIGLECNRS-CDST / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc