Impact of KRASG13D Mutation on Colorectal Cancer Cell Line HCT116 Organelle Structure, Function, and Growth

Undergraduate Theoretical Proposa

The Relationship Between KRAS and the Endoplasmic Reticulum-mitochondrial Calcium Flux in Isogenic Colorectal Cancer Cell Lines

Undergraduate Theoretical Proposa

The G13D KRAS Mutation’s Contribution to Structural and Functional Changes of the Endoplasmic Reticulum and Mitochondria in Colorectal Cancer Cells

Undergraduate Theoretical Proposa

Challenges in modeling total knee arthroplasty and total hip replacement

AbstractTotal knee arthroplasty and hip replacement are commonly used procedures in which an ailing knee or hip joint is replaced with a carefully engineered artificial joint. Multibody models of healthy knee and hip joints are used extensively to design artificial knee and joints. The quality of the replacement joint is thus intrinsically related to the quality of the multibody models. In this work, the quality of a kinematic knee model is assessed by comparing predicted knee kinematics to cadaveric knee kinematics under several patellar overstuffing conditions. In addition, the micro motion at the hip joint (between the femoral head and the acetabulum) under varying loads is experimentally measured using a cemented and a cementless cadaveric hip joint

Challenges in modeling total knee arthroplasty and total hip replacement

AbstractTotal knee arthroplasty and hip replacement are commonly used procedures in which an ailing knee or hip joint is replaced with a carefully engineered artificial joint. Multibody models of healthy knee and hip joints are used extensively to design artificial knee and joints. The quality of the replacement joint is thus intrinsically related to the quality of the multibody models. In this work, the quality of a kinematic knee model is assessed by comparing predicted knee kinematics to cadaveric knee kinematics under several patellar overstuffing conditions. In addition, the micro motion at the hip joint (between the femoral head and the acetabulum) under varying loads is experimentally measured using a cemented and a cementless cadaveric hip joint

Estrategias de regulación emocional en pacientes con cáncer

Los objetivos del presente trabajo son identificar si predomina la supresión de la expresión emocional por sobre otras estrategias en pacientes oncológicos, examinar si estos presentan una tendencia a expresar emociones negativas en menor medida que las positivas, evaluar la posible presencia de sintomatología ansiosa y depresiva, identificar factores de personalidad característicos y determinar si existe algún nivel de correlación entre las variables evaluadas. Para esto se evaluó a un grupo de 30 pacientes oncológicos con diversos tipos de patología cancerosa. Los resultados muestran que los pacientes utilizan la Reevaluación Cognitiva por sobre la supresión de la expresión emocional, además que expresan sus emociones positivas en mayor medida que las negativas. Por otro lado se observa que los pacientes no muestran índices significativos de sintomatología depresiva ni ansiosa. En cuanto a los factores de personalidad, los pacientes poseen puntajes altos para el factor “Agradabilidad”. Por último Depresión y Neuroticismo correlacionaron de manera positiva, expresividad negativa lo hizo con Supresión Emocional, Depresión y Neuroticismo. Este último correlacionó con síntomas ansiosos y síntomas depresivos, correlacionó positivamente con supresión emocional.The objectives of this study are to identify whether dominant suppression of emotional expression over other strategies in cancer patients, to examine whether these have a tendency to express negative emotions to a lesser extent than positive, evaluate the possible presence of anxiety, depression, identify characteristic personality factors and determine whether there is any level of correlation between the variables evaluated. To this was evaluated in a group of 30 cancer patients with different types of cancer pathology. The results show that patients use Cognitive Reappraisal over the suppression of emotional expression, besides expressing positive emotions to a greater extent than negative. On the other hand it is observed that the patients do not show significant levels of depressive and anxious symptoms. As for personality factors patients have higher scores for "agreeableness" factor. Finally Depression and Neuroticism correlated positively, negative expressivity did with Emotional Suppression, Depression and Neuroticism. The latter correlated with anxiety symptoms and depressive symptoms positively correlated with emotional suppression.Fil: Solitro, Meliss

Primary cup stability in THA with augmentation of acetabular defect. A comparison of healthy and osteoporotic bone

AbstractBackground contextReconstruction of acetabular defect has been advocated as standard procedure in total hip arthroplasty. The presence of bony defects at the acetabulum is viewed as a cause of instability and acetabular wall augmentation is often used without proper consideration of surrounding bone density. The initial cup-bone stability is, however, a challenge and a number of studies supported by clinical follow-ups of patients suggested that if the structural graft needs supporting more than 50% of the acetabular component, a reconstruction cage device spanning ilium to ischium should be preferred to protect the graft and provide structural stability. This study aims to (1) investigate the relationship between cup motion and bone density and (2) quantify the re-distribution of stress at the defect site after augmentation.HyphotesisPaprosky type I or II, acetabular defects, when reconstructed with bone screws supported by bioabsorbable calcified triglyceride bone cement are significantly less effective for osteoporotic bone than healthy bone.Materials and methodsAcetabular wall defects were reconstructed on six cadaveric subjects with bioabsorbable calcified triglyceride bone cement using a re-bar technique. Data of the specimen with higher bone density was used to validate a Finite Element Model. Values of bone apparent density ranging from healthy to osteoporotic were simulated to evaluate (1) the cup motion, through both displacement and rotation, (2) and the von Mises stress distribution.ResultsDefect reconstruction with bone screws and bioabsorbable calcified triglyceride bone cement results in a re-distribution of stress at the defect site. For a reduction of 65% in bone density, the cup displacement was similar to a healthy bone for loads not exceeding 300N, as load progressed up to 1500N, the reconstructed defect showed increase of 99μm (128%) in displacement and of 0.08° in rotation angle.ConclusionsBased on the results, we suggest that an alternative solution to wall defect augmentation with bone screws supported by bioabsorbable calcified triglyceride bone cement, be used for osteoporotic bone.Level of evidenceLevel IV, experimental and cadaveric study