<i>Origanum</i> species native to the island of Crete: <i>in vitro</i> antioxidant characteristics and liquid chromatography–mass spectrometry identification of major polyphenolic components

<div><p>Extracts from three <i>Origanum</i> species, including <i>Origanum microphyllum</i>, <i>Origanum dictamnus</i> and <i>Origanum vulgare</i> subsp. <i>hirtum</i>, native to the island of Crete (southern Greece), were partly fractionated through successive partition with ethyl acetate and <i>n</i>-butanol. All the fractions obtained were profiled with regard to their major polyphenolic constituents, using liquid chromatography-diode array-mass spectrometry. Furthermore, the antioxidant potency of each fraction was assessed by estimating the antiradical activity (<i>A</i>_AR) and the hydroxyl free radical scavenging activity (SA_HFR). The chromatographic analyses revealed a rich profile mainly for the ethyl acetate fractions, composed principally by flavones, which were accompanied by a limited number of phenylpropanoids, flavanones and dihydroflavonols. The highest values of antioxidant activity were displayed by the ethyl acetate extract of <i>O. dictamnus</i>, which also possessed the richest polyphenolic composition.</p></div

MOESM1 of Production of the forskolin precursor 11β-hydroxy-manoyl oxide in yeast using surrogate enzymatic activities

Additional file 1: Table S1. List of primers. Table S2. Experimental and bibliographic 1H and 13C NMR data (in CDCl3) of 11β-hydroxy-manoyl oxide (4). Figure S1. 1H NMR spectrum of 11β-hydroxy-manoyl oxide (4). Figure S2. 13C NMR spectrum of 11β-hydroxy-manoyl oxide (4)

Biological activities and secondary compound composition from <i>Crithmum maritimum</i> aerial parts

<p>This study aimed to assess the biological activities and secondary compound composition from Algerian <i>Crithmum maritimum</i> and, therefore, to better characterize local medicinal plants. The aerial parts were investigated regarding their mineral, antioxidant and essential oil content, and composition. The <i>C. maritimum</i> aerial parts contained high levels of potassium, calcium, manganese, and iron. The phenolic fraction was composed by at least 10 identified hydroxycinnamic acids that exhibited scavenging activity against DPPH and ABTS⁺. Moreover, the <i>C. maritimum</i> essential oil had a strong antimicrobial activity. The essential oil was composed by a least 10 molecules comprising monoterpene hydrocarbons and oxygenated monoterpenes.</p