5 research outputs found
Antioxidant activity of carotenoid lutein <i>in vitro</i> and <i>in vivo</i>
843-848Carotenoid lutein was evaluated for its antioxidant potential both in vitro and in vivo. Lutein was
found to scavenge superoxide radicals, hydroxyl radicals and inhibited in vitro lipid peroxidation.
Concentrations needed for 50 % inhibition (IC50) were 21, 1.75 and 2.2 g/mL respectively. It scavenged
2,2-diphenyl-1-picryl hydrazyl (IC50 35 g/mL) and
nitric oxide radicals (IC50 3.8 g/mL) while 2,2-azobis-3-ethylbenzthiozoline-6-sulfonic acid
radicals were inhibited at higher concentration. Ferric reducing power (50%) of
lutein was found to be equal 0.3μmols/mL
of FeSO4.7H2O. Its oral administration inhibited
superoxide generation in macrophages in
vivo by 34.18, 64.32 and 70.22 % at doses of 50, 100 and 250 mg/kg body
weight. The oral administration of lutein in mice for 1 month significantly
increased the activity of catalase, superoxide dismutase, glutathione reductase
and glutathione in blood and liver while the activity of glutathione peroxidase
and glutathione-S-transferase were found to be increased in the liver tissue.
Implication of these results in terms of its role in reducing degenerative
diseases is discussed
Hepato-protective potential of carotenoid <i style="">meso-</i>zeaxanthin against paracetamol, CCl<sub>4</sub> and ethanol induced toxicity
44-49Hepato-protective potential of carotenoid meso-zeaxanthin [(3R, 3’S)-,
-carotene-3, 3′-diol] was studied using
in vivo rat models. Paracetamol (3 g/kg
body wt, orally), 20% ethanol (7.5 g/kg body wt, orally) and CCl4 (2.5ml
/kg, ip) were used as hepato toxins. Levels of marker enzymes of hepatic injury
such as serum glutamate oxaloacetate transaminase, serum glutamate pyruvate
transaminase and alkaline phosphatase, and serum bilirubin, which were
drastically elevated by these hepato toxins were significantly decreased by meso-zeaxanthin pretreatment in a dose-dependent
manner. Oxidative stress markers, tissue lipid peroxidation, conjugated dienes
and tissue hydroperoxides, were high in the paracetamol treated control group
animals, which were lowered by meso-zeaxanthin
administration. Level of glutathione and antioxidant enzymes, superoxide
dismutase, catalase and glutathione peroxidase, in liver tissue was increased by
meso-zeaxanthin pretreatment compared
to control group during alcohol and CCl4 induced hepatotoxicity. Hydroxyproline,
an indicator of fibrosis in liver tissue, decreased remarkably by meso-zeaxanthin administration despite
its notable elevation in ethanol treated rats. Histopathological analysis of
liver tissue showed the hepatoprotective potential of meso-zeaxanthin